Transcript: Human NM_080817.5

Homo sapiens G protein-coupled receptor 82 (GPR82), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
GPR82 (27197)
Length:
3318
CDS:
215..1225

Additional Resources:

NCBI RefSeq record:
NM_080817.5
NBCI Gene record:
GPR82 (27197)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_080817.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000357280 GGTCATCCAGATTCTACTAAT pLKO_005 970 CDS 100% 13.200 18.480 N GPR82 n/a
2 TRCN0000008949 CCTCGCAAGAGACTACTTCAT pLKO.1 591 CDS 100% 4.950 6.930 N GPR82 n/a
3 TRCN0000357343 CATTCAAGAAGACACTATATA pLKO_005 1158 CDS 100% 15.000 10.500 N GPR82 n/a
4 TRCN0000357283 CCATGGCTTTACCAATCATTT pLKO_005 258 CDS 100% 13.200 9.240 N GPR82 n/a
5 TRCN0000008950 GCTTCCTTCCTTATAGTATTT pLKO.1 996 CDS 100% 13.200 9.240 N GPR82 n/a
6 TRCN0000357340 TCAATGCAGAGTGGTCAATTT pLKO_005 472 CDS 100% 13.200 9.240 N GPR82 n/a
7 TRCN0000008946 CCAGCCCAACTTTGCTAGAAA pLKO.1 655 CDS 100% 5.625 3.938 N GPR82 n/a
8 TRCN0000008948 CCTATGCTACAATCGGCAGAT pLKO.1 769 CDS 100% 4.050 2.835 N GPR82 n/a
9 TRCN0000008947 CCAATCATTTACATCCTCCTT pLKO.1 269 CDS 100% 2.640 1.848 N GPR82 n/a
10 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 1747 3UTR 100% 4.950 2.475 Y ORAI2 n/a
11 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 1668 3UTR 100% 4.950 2.475 Y ERAP2 n/a
12 TRCN0000164885 GCTGAGGCAGAAGAATCACTT pLKO.1 2804 3UTR 100% 4.950 2.475 Y FAM74A4 n/a
13 TRCN0000027826 CCGCTATGCTACCTTAATGAA pLKO.1 562 CDS 100% 5.625 3.938 N Gpr82 n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 1669 3UTR 100% 13.200 6.600 Y LIAS n/a
15 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 1744 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_080817.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489463 TGACGCTAGCAGGTGCGCATAAGT pLX_317 37.8% 100% 100% V5 (not translated due to prior stop codon) n/a
2 TRCN0000488235 CGTGCCTCGTCCCGATTTAGCTAT pLX_317 37.5% 99.9% 99.7% V5 1008_1009insG n/a
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