Construct: ORF TRCN0000488316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021590.1_s317c1
- DNA Barcode:
- GGGACAACGTGGCTATACCTCCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DDX18 (8886)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488316
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8886 | DDX18 | DEAD-box helicase 18 | NM_006773.4 | 99.9% | 99.8% | 281C>G |
| 2 | mouse | 66942 | Ddx18 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_025860.3 | 81.1% | 86.1% | (many diffs) |
| 3 | mouse | 66942 | Ddx18 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_006529794.3 | 74% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2079
- ORF length:
- 2010
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtcacacctg ccgatgaaac tcctgcgtaa gaagatcgag aagcggaacc 121 tcaaattgcg gcagcggaac ctaaagtttc agggggcctc aaatctgacc ctatcggaaa 181 ctcaaaatgg agatgtatct gaagaaacaa tgggaagtag aaaggttaaa aaatcaaaac 241 aaaagcccat gaatgtgggc ttatcagaaa ctcaaaatgg aggcatgtct caagaagcag 301 tgggaaatat aaaagttaca aagtctcccc agaaatccac tgtattaagc aatggagaag 361 cagcaatgca gtcttccaat tcagaatcaa aaaagaaaaa gaagaaaaag agaaaaatgg 421 tgaatgatgc tgagcctgat acgaaaaaag caaaaactga aaacaaaggg aaatctgaag 481 aagaaagtgc cgagactact aaagaaacag aaaataatgt ggagaagcca gataatgatg 541 aagatgagag tgaggtgccc agtctgcccc tgggactgac aggagctttt gaggatactt 601 cgtttgcttc tctatgtaat cttgtcaatg aaaacactct gaaggcaata aaagaaatgg 661 gttttacaaa catgactgaa attcagcata aaagtatcag accacttctg gaaggcaggg 721 atcttctagc agctgcaaaa acaggcagtg gtaaaaccct ggcttttctc atccctgcag 781 ttgaactcat tgttaagtta aggttcatgc ccaggaatgg aacaggagtc cttattctct 841 cacctactag agaactagcc atgcaaacct ttggtgttct taaggagctg atgactcacc 901 acgtgcatac ctatggcttg ataatgggtg gcagtaacag atctgctgaa gcacagaaac 961 ttggtaatgg gatcaacatc attgtggcca caccaggccg tctgctggac catatgcaga 1021 ataccccagg atttatgtat aaaaacctgc agtgtctggt tattgatgaa gctgatcgta 1081 tcttggatgt ggggtttgaa gaggaattaa agcaaattat taaacttttg ccaacacgta 1141 gacagactat gctcttttct gccacccaaa ctcgaaaagt tgaagacctg gcaaggattt 1201 ctctgaaaaa ggagccattg tatgttggcg ttgatgatga taaagcgaat gcaacagtgg 1261 atggtcttga acagggatat gttgtttgtc cttctgaaaa gagattcctt ctgctcttta 1321 cattccttaa gaagaaccga aagaagaagc ttatggtctt cttttcatct tgtatgtctg 1381 tgaaatacca ctatgagttg ctgaactaca ttgatttgcc cgtcttggcc attcatggaa 1441 agcaaaagca aaataagcgt acaaccacat tcttccagtt ctgcaatgca gattcgggaa 1501 cactattgtg tacggatgtg gcagcgagag gactagacat tcctgaagtc gactggattg 1561 ttcagtatga ccctccggat gaccctaagg aatatattca tcgtgtgggt agaacagcca 1621 gaggcctaaa tgggagaggg catgccttgc tcattttgcg cccagaagaa ttgggttttc 1681 ttcgctactt gaaacaatcc aaggttccat taagtgaatt tgacttttcc tggtctaaaa 1741 tttctgacat tcagtctcag cttgagaaat tgattGAAAA GAATTACTTT CTTCATAAGT 1801 CAGCCCAGGA AGCATATAAG TCATACATAC GAGCCTATGA TTCCCATTCT CTGAAACAGA 1861 TCTTTAATGT TAATAACCTA AATTTGCCTC AGGTTGCTCT GTCATTTGGT TTCAAGGTGC 1921 CTCCCTTCGT TGATCTGAAC GTCAACAGTA ATGAAGGCAA GCAGAAAAAG CGAGGAGGTG 1981 GTGGTGGATT TGGCTACCAG AAAACCAAGA AAGTTGAGAA ATCCAAAATC TTTAAACACA 2041 TTAGCAAGAA ATCATCTGAC AGCAGGCAGT TCTCTCACTA GAACCCAGCT TTCTTGTACA 2101 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2161 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2221 AGGACGAGGG ACAACGTGGC TATACCTCCA TACGCGTTAA GTCgacaatc aacctctgga 2281 ttacaaaatt tgtgaaagat t