Construct: ORF TRCN0000488341
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022016.1_s317c1
- DNA Barcode:
- CTACCCCCGCGTCGCTGCAATTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHRFAM7A (89832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488341
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021884.1 | 91.6% | 91.9% | (many diffs) |
| 2 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | NM_148911.1 | 91.5% | 91.9% | (many diffs) |
| 3 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_005254750.3 | 91.5% | 91.9% | (many diffs) |
| 4 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521177.2 | 86.7% | 84.1% | (many diffs) |
| 5 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | NM_139320.1 | 84.3% | 81.8% | (many diffs) |
| 6 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_011522153.2 | 84.3% | 81.8% | (many diffs) |
| 7 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021883.2 | 79% | 76.6% | (many diffs) |
| 8 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021882.1 | 78.9% | 76.5% | (many diffs) |
| 9 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521176.3 | 71.6% | 69.4% | (many diffs) |
| 10 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NM_000746.6 | 69.3% | 67.2% | (many diffs) |
| 11 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NM_001190455.3 | 65.5% | 63.6% | (many diffs) |
| 12 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_011522155.3 | 54.8% | 37.8% | (many diffs) |
| 13 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521178.3 | 45.1% | 31.1% | (many diffs) |
| 14 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NR_046324.1 | 32.9% | (many diffs) | |
| 15 | mouse | 11441 | Chrna7 | cholinergic receptor, nicot... | NM_007390.3 | 60.2% | 62.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1116
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtacgctggt ttccctttga tgtgcagcac tgcaaacaag tttgggtcct 121 ggtcttacgg aggctggtcc ttggatctgc agatgcagga ggcagatatc agtggctata 181 tccccaatgg agaatgggac ctagtgggaa tccccggcaa gaggagtgaa aggttctatg 241 agtgctgcaa agagccctac cccgatgtca ccttcacagt gaccatgcgc cgcaggacac 301 tctactatgg cctcaacctg ctgatcccct gtgtgctcat ctccgccctc gccctgctgg 361 tgttcctgct tcctgcagat tccggggaga agatttccct ggggataaca gtcttactct 421 ctcttaccgt cttcatgctg ctcgtggctg agatcatgcc cgcaacatcc gattcggtac 481 cattgatagc ccagtacttc gccagcacca tgatcatcgt gggcctctcg gtggtggtga 541 cggtgatcgt gctgcagtac caccaccacg accccgacgg gggcaagatg cccaagtgga 601 ccagagtcat ccttctgaac tggtgcgcgt ggttcctgcg aatgaagagg cccggggagg 661 acaaggtgcg cccggcctgc cagcacaagc agcggcgctg cagcctggcc agtgtggaga 721 tgagcgccgt ggcgccgccg cccgccagca acgggaacct gctgtacatc ggcttccgcg 781 gcctggacgg cgtgcactgt gtcccgaccc ccgactctgg ggtagtgtgt ggccgcatgg 841 cctgctcccc cacgcacgat gagcaccTCC TGCACGGTGG GCAACCCCCC GAGGGGGACC 901 CGGACTTGGC CAAGATCCTG GAGGAGGTCC GCTACATTGC CAACCGCTTC CGCTGCCAGG 961 ACGAAAGCGA GGCGGTCTGC AGCGAGTGGA AGTTCGCCGC CTGTGTGGTG GACCGCCTGT 1021 GCCTCATGGC CTTCTCGGTC TTCACCATCA TCTGCACCAT CGGCATCCTG ATGTCGGCTC 1081 CCAACTTCGT GGAGGCCGTG TCCAAAGACT TTGCGTAAGA CCCAGCTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGACTACCC CCGCGTCGCT GCAATTCCAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt