Construct: ORF TRCN0000488347

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021194.1_s317c1
DNA Barcode:
AGTGCCATTTAGCATCCTCTTTTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
OPCML (4978)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488347
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4978 OPCML opioid binding protein/cell... NM_002545.4 100% 100%
2 human 4978 OPCML opioid binding protein/cell... NM_001319106.1 99.7% 99.7% 525_526insGAA
3 human 4978 OPCML opioid binding protein/cell... NM_001319103.1 97.4% 97.4% 937_963del
4 human 4978 OPCML opioid binding protein/cell... NM_001012393.3 95% 93.3% (many diffs)
5 human 4978 OPCML opioid binding protein/cell... XM_006718846.3 94.7% 93% (many diffs)
6 human 4978 OPCML opioid binding protein/cell... NM_001319105.1 88.1% 88.1% 0_1ins123
7 human 4978 OPCML opioid binding protein/cell... XM_011542856.3 88.1% 88.1% 0_1ins123
8 human 4978 OPCML opioid binding protein/cell... NM_001319104.2 71% 71% 0_1ins300
9 mouse 330908 Opcml opioid binding protein/cell... XM_006510437.3 92% 98.5% (many diffs)
10 mouse 330908 Opcml opioid binding protein/cell... XM_006510438.3 91.7% 98.2% (many diffs)
11 mouse 330908 Opcml opioid binding protein/cell... XM_006510434.3 89.7% 96% (many diffs)
12 mouse 330908 Opcml opioid binding protein/cell... XM_006510435.3 89.4% 95.7% (many diffs)
13 mouse 330908 Opcml opioid binding protein/cell... NM_177906.4 86.9% 91.5% (many diffs)
14 mouse 330908 Opcml opioid binding protein/cell... XM_011242559.2 85% 89.5% (many diffs)
15 mouse 330908 Opcml opioid binding protein/cell... XM_006510439.3 78.4% 84.4% (many diffs)
16 mouse 330908 Opcml opioid binding protein/cell... XM_006510440.2 62.9% 67.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1107
ORF length:
1035
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgggggtc tgtgggtacc tgttcctgcc ctggaagtgc ctcgtggtcg
121tgtctctcag gctgctgttc cttgtaccca caggagtgcc cgtgcgcagc ggagatgcca
181ccttccccaa agctatggac aacgtgacgg tccggcaggg ggagagcgcc accctcaggt
241gtaccataga tgaccgggta acccgggtgg cctggctaaa ccgcagcacc atcctctacg
301ctgggaatga caagtggtcc atagaccctc gtgtgatcat cctggtcaat acaccaaccc
361agtacagcat catgatccaa aatgtggatg tgtatgacga aggtccgtac acctgctctg
421tgcagacaga caatcatccc aaaacgtccc gggttcacct aatagtgcaa gttcctcctc
481agatcatgaa tatctcctca gacatcactg tgaatgaggg aagcagtgtg accctgctgt
541gtcttgctat tggcagacca gagccaactg tgacatggag acacctgtca gtcaaggaag
601gccagggctt tgtaagtgag gatgagtacc tggagatctc tgacatcaag cgagaccagt
661ccggggagta cgaatgcagc gcgttgaacg atgtcgctgc gcccgatgtg cggaaagtaa
721aaatcactgt aaactatcct ccctatatct caaaagccaa gaacactggt gtttcagtcg
781gtcagaaggg catccTGAGC TGTGAAGCCT CTGCAGTCCC CATGGCTGAA TTCCAGTGGT
841TCAAGGAAGA AACCAGGTTA GCCACTGGTC TGGATGGAAT GAGGATTGAA AACAAAGGCC
901GCATGTCCAC TCTGACTTTC TTCAATGTTT CAGAAAAGGA TTATGGGAAC TATACTTGTG
961TGGCCACGAA CAAGCTTGGG AACACCAATG CCAGCATCAC ATTGTATGGG CCTGGAGCAG
1021TCATTGATGG TGTAAACTCG GCCTCCAGAG CACTGGCTTG TCTCTGGCTA TCAGGGACCC
1081TCTTAGCCCA CTTCTTCATC AAGTTTTGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA
1141TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1201CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGTGC
1261CATTTAGCAT CCTCTTTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1321tgaaagatt

Download FASTA (ORF) (Full)