Construct: ORF TRCN0000488354
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020707.1_s317c1
- DNA Barcode:
- ATATTTCAACAAGCACACTGTCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CILK1 (22858)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488354
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22858 | CILK1 | ciliogenesis associated kin... | NM_014920.4 | 100% | 100% | |
| 2 | human | 22858 | CILK1 | ciliogenesis associated kin... | NM_016513.4 | 100% | 100% | |
| 3 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_017010488.1 | 100% | 100% | |
| 4 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_017010489.1 | 100% | 100% | |
| 5 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_017010490.1 | 100% | 100% | |
| 6 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_017010491.1 | 100% | 100% | |
| 7 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_017010492.1 | 100% | 100% | |
| 8 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_011514419.1 | 98.9% | 98.9% | 1153_1173del |
| 9 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_011514420.2 | 98.9% | 98.9% | 1153_1173del |
| 10 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_011514421.1 | 98.9% | 98.9% | 1153_1173del |
| 11 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_017010485.1 | 98.9% | 98.9% | 1153_1173del |
| 12 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_017010486.1 | 98.9% | 98.9% | 1153_1173del |
| 13 | human | 22858 | CILK1 | ciliogenesis associated kin... | XM_017010487.1 | 98.9% | 98.9% | 1153_1173del |
| 14 | mouse | 56542 | Ick | intestinal cell kinase | NM_001163780.1 | 84.5% | 86.3% | (many diffs) |
| 15 | mouse | 56542 | Ick | intestinal cell kinase | NM_019987.2 | 84.5% | 86.3% | (many diffs) |
| 16 | mouse | 56542 | Ick | intestinal cell kinase | XM_006511304.2 | 84.5% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1968
- ORF length:
- 1896
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaataga tacacaacaa tcaggcagct cggggatgga acctacggtt 121 ccgtcctgct gggaagaagc attgagtctg gggagctgat cgctattaaa aaaatgaaaa 181 gaaaatttta ttcctgggag gaatgcatga accttcggga ggttaagtct ttaaagaagc 241 tcaaccatgc caatgtagtc aaattaaaag aagttatcag ggaaaatgat catctttatt 301 ttatcttcga gtacatgaag gaaaatcttt accagctcat taaagagaga aataagttgt 361 ttcctgagtc tgctataagg aatatcatgt atcagatatt acaaggactc gcatttattc 421 acaaacacgg cttctttcat cgagacttaa agcctgagaa cctcctctgc atgggaccag 481 aacttgtgaa aattgcagac tttggtttgg cccgagaaat acgatcaaaa cctccatata 541 cagattatgt atctaccaga tggtacaggg ctccagaagt actcctgagg tctaccaact 601 acagctcccc cattgacgtc tgggcggtgg gctgcatcat ggcagaagtt tacaccctca 661 ggccactctt ccctggagcc agtgaaattg acacaatatt caaaatttgc caagtgctgg 721 ggacaccaaa aaagactgac tggcctgaag gctatcaact ttcaagtgca atgaacttcc 781 gttggccaca gtgtgtaccc aataacttaa agaccttgat tcccaatgct agcagtgaag 841 cagtccagct cctgagagac atgcttcagt gggatcccaa gaaacgacca acagctagtc 901 aggcacttcg atatccttac ttccaagttg gacacccact aggcagcacc acacaaaacc 961 ttcaggattc agaaaaacca cagaaaggca tcctggaaaa ggcaggccca cctccttata 1021 ttaagccagt cccacctgcc cagccaccag ccaagccaca cacacgaatt tcttcacgac 1081 agcatcaagc cagccagccc cctctgcatc tcacgtaccc ctacaaagca gaggtctcca 1141 ggacagatca cccaagccat ctccaggagg acaagccaag cccgttgctt ttcccatccc 1201 tccacaacaa gcatccacag tcgaaaatca cagctggcct ggagcacaaa aatggtgaga 1261 taaagccaaa gagtaggaga aggtggggtc ttatttccag gtcaacaaag gattcagatg 1321 attgggctga cttggatgac ttggatttca gtccatccct cagcaggatt gacctgaaaa 1381 acaagaaaag acagagtgat gacactctct gcaggtttga gagtgttttg gacctgaagc 1441 cctctgagcc tgtgggcaca ggaaacagtg cccccaccca gacgtcatat cagcggcgag 1501 acacgcccac cctgagatct gcagccaagc agcactattt gaagcactct cgatacttgc 1561 ctgggatcag tataagaaat ggcatactct cgaatccagg caaggaattt attccaccTA 1621 ATCCATGGTC TAGTTCTGGC TTGTCTGGAA AATCTTCAGG GACAATGTCA GTAATCAGCA 1681 AAGTAAATTC AGTTGGTTCC AGCTCTACAA GTTCTAGTGG ACTGACTGGA AACTATGTCC 1741 CTTCCTTTCT GAAAAAAGAA ATCGGTTCTG CTATGCAGAG GGTACACCTA GCACCTATTC 1801 CAGACCCTTC CCCTGGTTAT TCCTCCCTGA AGGCCATGAG ACCTCATCCT GGGCGACCAT 1861 TCTTCCACAC CCAGCCTAGA AGCACTCCTG GGTTGATACC ACGGCCTCCA GCCGCCCAGC 1921 CAGTGCATGG CCGGACAGAC TGGGCTTCCA AGTACGCATC TCGGCGATAG AACCCAGCTT 1981 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2041 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2101 CTTGTGGAAA GGACGAATAT TTCAACAAGC ACACTGTCCA ACGCGTTAAG TCgacaatca 2161 acctctggat tacaaaattt gtgaaagatt