Construct: ORF TRCN0000488374
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020531.1_s317c1
- DNA Barcode:
- CTGCGTATTCCCTACAAAGGGGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PAK4 (10298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488374
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014831.3 | 100% | 100% | |
| 2 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014832.2 | 100% | 100% | |
| 3 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_005884.4 | 100% | 100% | |
| 4 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526316.1 | 100% | 100% | |
| 5 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526317.2 | 100% | 100% | |
| 6 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526318.2 | 100% | 100% | |
| 7 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526319.2 | 100% | 100% | |
| 8 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014834.3 | 74.1% | 74.1% | 202_203ins459 |
| 9 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014835.2 | 74.1% | 74.1% | 202_203ins459 |
| 10 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526320.1 | 74.1% | 74.1% | 202_203ins459 |
| 11 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_024451314.1 | 74.1% | 74.1% | 202_203ins459 |
| 12 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | NM_027470.3 | 86.2% | 92.5% | (many diffs) |
| 13 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312296.1 | 86.2% | 92.5% | (many diffs) |
| 14 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312297.1 | 86.2% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1845
- ORF length:
- 1773
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtttggg aagaggaaga agcgggtgga gatctccgcg ccgtccaact 121 tcgagcaccg cgtgcacacg ggcttcgacc agcacgagca gaagttcacg gggctgcccc 181 gccagtggca gagcctgatc gaggagtcgg ctcgccggcc caagcccctc gtcgaccccg 241 cctgcatcac ctccatccag cccggggccc ccaagaccat cgtgcggggc agcaaaggtg 301 ccaaagatgg ggccctcacg ctgctgctgg acgagtttga gaacatgtcg gtgacacgct 361 ccaactccct gcggagagac agcccgccgc cgcccgcccg tgcccgccag gaaaatggga 421 tgccagagga gccggccacc acggccagag ggggcccagg gaaggcaggc agccgaggcc 481 ggttcgccgg tcacagcgag gcgggtggcg gcagtggtga caggcgacgg gcggggccag 541 agaagaggcc caagtcttcc agggagggct cagggggtcc ccaggagtcc tcccgggaca 601 aacgccccct ctccgggcct gatgtcggca ccccccagcc tgctggtctg gccagtgggg 661 cgaaactggc agctggccgg ccctttaaca cctacccgag ggctgacacg gaccacccat 721 cccggggtgc ccagggggag cctcatgacg tggcccctaa cgggccatca gcggggggcc 781 tggccatccc ccagtcctcc tcctcctcct cccggcctcc cacccgagcc cgaggtgccc 841 ccagccctgg agtgctggga ccccacgcct cagagcccca gctggcccct ccagcctgca 901 cccccgccgc ccctgctgtt cctgggcccc ctggcccccg ctcaccacag cgggagccac 961 agcgagtatc ccatgagcag ttccgggctg ccctgcagct ggtggtggac ccaggcgacc 1021 cccgctccta cctggacaac ttcatcaaga ttggcgaggg ctccacgggc atcgtgtgca 1081 tcgccaccgt gcgcagctcg ggcaagctgg tggccgtcaa gaagatggac ctgcgcaagc 1141 agcagaggcg cgagctgctc ttcaacgagg tggtaatcat gagggactac cagcacgaga 1201 atgtggtgga gatgtacaac agctacctgg tgggggacga gctctgggtg gtcatggagt 1261 tcctggaagg aggcgccctc accgacatcg tcacccacac caggatgaac gaggagcaga 1321 tcgcggccgt gtgccttgca gtgctgcagg ccctgtcggt gctccacgcc cagggcgtca 1381 tccaccggga catcaagagc gactcgatcc tgctgaccca tgatggcagg gtgaagctgt 1441 cagactttgg gttctgcgcc caggtgagca aggaagtgcc ccgaaggaag tcgctggtcg 1501 gcacgcccta ctggatggcc ccagagctca tctcccgcct tcccTACGGG CCAGAGGTAG 1561 ACATCTGGTC GCTGGGGATA ATGGTGATTG AGATGGTGGA CGGAGAGCCC CCCTACTTCA 1621 ACGAGCCACC CCTCAAAGCC ATGAAGATGA TTCGGGACAA CCTGCCACCC CGACTGAAGA 1681 ACCTGCACAA GGTGTCGCCA TCCCTGAAGG GCTTCCTGGA CCGCCTGCTG GTGCGAGACC 1741 CTGCCCAGCG GGCCACGGCA GCCGAGCTGC TGAAGCACCC ATTCCTGGCC AAGGCAGGGC 1801 CGCCTGCCAG CATCGTGCCC CTCATGCGCC AGAACCGCAC CAGATAGAAC CCAGCTTTCT 1861 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1921 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1981 GTGGAAAGGA CGACTGCGTA TTCCCTACAA AGGGGTAACG CGTTAAGTCg acaatcaacc 2041 tctggattac aaaatttgtg aaagatt