Construct: ORF TRCN0000488375
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020303.1_s317c1
- DNA Barcode:
- TTCTCACGTAATATCCGCCAGCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK26 (51765)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488375
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_001042452.2 | 100% | 100% | |
| 2 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_016542.4 | 85% | 85% | 598_783del |
| 3 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_001042453.2 | 66.5% | 66.5% | 40_41ins231;367_552del |
| 4 | human | 51765 | STK26 | serine/threonine kinase 26 | XM_011531349.1 | 61.5% | 61.5% | 0_1ins294;304_489del |
| 5 | human | 51765 | STK26 | serine/threonine kinase 26 | XM_011531350.1 | 60% | 57.2% | (many diffs) |
| 6 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | NM_133729.2 | 77.4% | 83.4% | (many diffs) |
| 7 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | NM_001313744.1 | 72.8% | 70.8% | (many diffs) |
| 8 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | XM_017318618.1 | 60.2% | 64.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1134
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcccac tcgccggtgg ctgtccaagt gcctgggatg cagaataaca 121 tagctgatcc agaagaactg ttcacaaaat tagagcgcat tgggaaaggc tcatttgggg 181 aagttttcaa aggaattgat aaccgtaccc agcaagtcgt tgctattaaa atcatagacc 241 ttgaggaagc cgaagatgaa atagaagaca ttcagcaaga aataactgtc ttgagtcaat 301 gtgacagctc atatgtaaca aaatactatg ggtcatattt aaaggggtct aaattatgga 361 taataatgga atacctgggc ggtggttcag cactggatct tcttcgagct ggtccatttg 421 atgagttcca gattgctacc atgctaaagg aaattttaaa aggtctggac tatctgcatt 481 cagaaaagaa aattcaccga gacataaaag ctgccaatgt cttgctctca gaacaaggag 541 atgttaaact tgctgatttt ggagttgctg gtcagctgac agatacacag attaaaagaa 601 atacctttgt gggaactcca ttttggatgg ctcctgaagt tattcaacag tcagcttatg 661 actcaaaacg tcctacagca aaagaacttc tgaaacacaa attcattgta aaaaattcaa 721 agaagacttc ttatctgact gaactgatag atcgttttaa gagatggaag gcagaaggac 781 acagtgatga tgaatctgat tccgagggct ctgattcgga atctaccagc agggaaaaCA 841 ATACTCATCC TGAATGGAGC TTTACCACCG TACGAAAGAA GCCTGATCCA AAGAAAGTAC 901 AGAATGGGGC AGAGCAAGAT CTTGTGCAAA CCCTGAGTTG TTTGTCTATG ATAATCACAC 961 CTGCATTTGC TGAACTTAAA CAGCAGGACG AGAATAACGC TAGCAGGAAT CAGGCGATTG 1021 AAGAACTCGA GAAAAGTATT GCTGTGGCTG AAGCCGCCTG TCCCGGCATC ACAGATAAAA 1081 TGGTGAAGAA ACTAATTGAA AAATTTCAAA AGTGTTCAGC AGACGAATCC CCCTGAGACC 1141 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GATTCTCACG TAATATCCGC CAGCGCACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt