Construct: ORF TRCN0000488393
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021863.1_s317c1
- DNA Barcode:
- CCCAGCTCGCCGCAGATGCAGCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KCNC4 (3749)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488393
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3749 | KCNC4 | potassium voltage-gated cha... | NM_004978.5 | 100% | 100% | |
| 2 | human | 3749 | KCNC4 | potassium voltage-gated cha... | NM_001039574.3 | 97.3% | 94.4% | (many diffs) |
| 3 | human | 3749 | KCNC4 | potassium voltage-gated cha... | XM_006710625.4 | 96.8% | 96% | (many diffs) |
| 4 | human | 3749 | KCNC4 | potassium voltage-gated cha... | XM_006710627.4 | 96% | 96.5% | (many diffs) |
| 5 | human | 3749 | KCNC4 | potassium voltage-gated cha... | XM_011541401.3 | 95.7% | 95.5% | 1823_1825delAGAinsCAT;1827_1828ins78 |
| 6 | human | 3749 | KCNC4 | potassium voltage-gated cha... | XM_024446790.1 | 94% | 92.7% | (many diffs) |
| 7 | human | 3749 | KCNC4 | potassium voltage-gated cha... | XM_011541404.2 | 47.8% | 34.9% | (many diffs) |
| 8 | human | 3749 | KCNC4 | potassium voltage-gated cha... | XM_011541402.2 | 45.1% | 34.3% | 677_678ins937;969_1209del |
| 9 | human | 3749 | KCNC4 | potassium voltage-gated cha... | XM_011541403.2 | 45.1% | 34.3% | 677_678ins937;969_1209del |
| 10 | human | 3749 | KCNC4 | potassium voltage-gated cha... | NR_036437.2 | 42.1% | 1_1186del;3092_4516del | |
| 11 | mouse | 99738 | Kcnc4 | potassium voltage gated cha... | NM_001356447.1 | 86.4% | 91% | (many diffs) |
| 12 | mouse | 99738 | Kcnc4 | potassium voltage gated cha... | NM_145922.3 | 85.3% | 93.1% | (many diffs) |
| 13 | mouse | 99738 | Kcnc4 | potassium voltage gated cha... | XM_006502475.4 | 83.1% | 89.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1974
- ORF length:
- 1905
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gatcagctcg gtgtgtgtct cctcctaccg cgggcgcaag tcggggaaca 121 agcctccgtc caaaacatgt ctgaaggagg agatggccaa gggcgaggcg tcggagaaga 181 tcatcatcaa cgtgggcggc acgcgacatg agacctaccg cagcaccctg cgcaccctac 241 cgggaacccg cctcgcctgg ctggccgacc ccgacggcgg gggccggccc gagaccgatg 301 gcggcggtgt gggtagcagc ggcagcagcg gcggcggggg ctgcgagttc ttcttcgaca 361 ggcacccggg cgtcttcgcc tacgtgctca actactaccg caccggcaag ctgcactgcc 421 ccgcggacgt gtgcgggccg ctcttcgaag aggagctcac cttctggggc atcgacgaga 481 ccgacgtgga accctgctgc tggatgacct accggcagca ccgcgacgcc gaggaggcgc 541 tcgacatctt cgagagcccg gacggaggcg gcagcggcgc ggggcccagc gacgaggccg 601 gcgacgatga gcgggagctg gccctgcagc gactgggccc ccacgaggga ggcgcgggcc 661 atggcgccgg gtctgggggc tgccgcggct ggcagccccg catgtgggcg ctcttcgagg 721 atccctactc ctcccgggcc gctagggtag tggcctttgc ctctctcttc ttcatcctgg 781 tctccatcac cactttctgc ctggagaccc atgaggcctt taatatcgac cgcaacgtga 841 cagagatcct ccgcgtaggg aacatcacca gcgtgcactt ccggcgggag gtagagacag 901 agcccatcct gacctacatc gagggcgtat gtgtgctgtg gttcacactg gagttcctgg 961 tgcgcatcgt gtgctgcccc gacacgctgg acttcgtcaa gaacctgctc aacatcatcg 1021 actttgtggc catcctgccc ttctacctgg aggtggggct gagcggcctg tcatccaagg 1081 cggcccgcga cgtgctgggc ttcctgcgcg tggtgcgctt cgtgcgcatc ctgcgtatct 1141 tcaagctcac acgccacttc gtggggctac gcgtgctggg ccacaccctg agggccagca 1201 ccaatgagtt cctgctgctt atcatcttcc tggccctggg tgtgctcatc tttgccacca 1261 tgatctacta cgctgagcgc attggggcca ggccctccga ccctcggggt aatgaccaca 1321 ccgacttcaa gaacatcccc attggcttct ggtgggctgt ggtcaccatg acgacactgg 1381 gctacggaga catgtacccc aagacgtggt caggcatgct ggtaggggca ctgtgtgcac 1441 tggctggcgt gctcaccatc gccatgccgg tgcctgtcat cgtcaacaac ttcggcatgt 1501 actactccct ggccatggcc aagcagaagc tgcccaagaa acggaagaag cacgtgccac 1561 ggccggcgca gctggagtca cccatgtact gcaagtctga ggagacttcc ccccgggaca 1621 gcaccTGCAG TGATACCAGC CCCCCTGCCC GGGAAGAGGG TATGATCGAG AGGAAACGGG 1681 CAGACTCTAA GCAGAATGGC GATGCCAACG CAGTGCTGTC TGATGAGGAG GGAGCTGGCC 1741 TCACCCAACC CCTGGCCTCC TCCCCGACCC CCGAGGAGCG CCGGGCCCTG CGACGCTCCA 1801 CCACTCGAGA CAGAAACAAG AAGGCAGCTG CCTGCTTCCT GCTCAGCACT GGGGACTATG 1861 CCTGCGCCGA TGGTAGTGTC CGGAAAGGCA CATTCGTCCT CCGTGACCTT CCCCTTCAGC 1921 ATTCACCTGA GGCTGCATGC CCTCCAACTG CTGGGACTCT GTTCCTGCCA CATTGAGACC 1981 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2041 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2101 ATATATCTTG TGGAAAGGAC GACCCAGCTC GCCGCAGATG CAGCCAACGC GTTAAGTCga 2161 caatcaacct ctggattaca aaatttgtga aagatt