Construct: ORF TRCN0000488417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020006.1_s317c1
- DNA Barcode:
- ATGCTCAACAGCCAACGAACTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPARD (5467)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5467 | PPARD | peroxisome proliferator act... | NM_177435.3 | 99.8% | 99.7% | 489C>T;1083_1084insG |
| 2 | human | 5467 | PPARD | peroxisome proliferator act... | XM_024446474.1 | 98.6% | 98.9% | (many diffs) |
| 3 | human | 5467 | PPARD | peroxisome proliferator act... | NM_001171818.2 | 81.7% | 81.4% | (many diffs) |
| 4 | human | 5467 | PPARD | peroxisome proliferator act... | NM_006238.5 | 81.7% | 81.4% | (many diffs) |
| 5 | human | 5467 | PPARD | peroxisome proliferator act... | XM_005249193.1 | 81.7% | 81.4% | (many diffs) |
| 6 | human | 5467 | PPARD | peroxisome proliferator act... | XM_006715120.1 | 81.7% | 81.4% | (many diffs) |
| 7 | human | 5467 | PPARD | peroxisome proliferator act... | XM_006715123.1 | 81.7% | 81.4% | (many diffs) |
| 8 | human | 5467 | PPARD | peroxisome proliferator act... | XM_011514707.1 | 81.7% | 81.4% | (many diffs) |
| 9 | human | 5467 | PPARD | peroxisome proliferator act... | XM_011514710.1 | 81.7% | 81.4% | (many diffs) |
| 10 | human | 5467 | PPARD | peroxisome proliferator act... | XM_017010972.1 | 81.7% | 81.4% | (many diffs) |
| 11 | human | 5467 | PPARD | peroxisome proliferator act... | XM_017010973.1 | 81.7% | 81.4% | (many diffs) |
| 12 | human | 5467 | PPARD | peroxisome proliferator act... | XM_017010974.1 | 81.7% | 81.4% | (many diffs) |
| 13 | human | 5467 | PPARD | peroxisome proliferator act... | NM_001171819.1 | 72.7% | 71.6% | (many diffs) |
| 14 | human | 5467 | PPARD | peroxisome proliferator act... | NM_001171820.1 | 59.4% | 59.1% | (many diffs) |
| 15 | mouse | 19015 | Ppard | peroxisome proliferator act... | NM_011145.3 | 73% | 75.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1158
- ORF length:
- 1086
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagcag ccacaggagg aagcccctga ggtccgggaa gaggaggaga 121 aagaggaagt ggcagaggca gaaggagccc cagagctcaa tgggggacca cagcatgcac 181 ttccttccag cagctacaca gacctctccc ggagctcctc gccaccctca ctgctggacc 241 aactgcagat gggctgtgac ggggcctcat gcggcagcct caacatggag tgccgggtgt 301 gcggggacaa ggcatcgggc ttccactacg gtgttcatgc atgtgagggg tgcaagggct 361 tcttccgtcg tacgatccgc atgaagctgg agtacgagaa gtgtgagcgc agctgcaaga 421 ttcagaagaa gaaccgcaac aagtgccagt actgccgctt ccagaagtgc ctggcactgg 481 gcatgtcaca caacgctatc cgttttggtc ggatgccgga ggctgagaag aggaagctgg 541 tggcagggct gactgcaaat gaggggagcc agtacaaccc acaggtggcc gacctgaagg 601 ccttctccaa gcacatctac aatgcctacc tgaaaaactt caacatgacc aaaaagaagg 661 cccgcagcat cctcaccggc aaagccagcc acacggcgcc ctttgtgatc cacgacatcg 721 agacattgtg gcaggcagag aaggggctgg tgtggaagca gttggtgaat ggcctgcctc 781 cctacaagga gatcagcgtg cacgtcttct accgctgcca gtgcaccaca gtggagaccg 841 tgcgGGAGCT CACTGAGTTC GCCAAGAGCA TCCCCAGCTT CAGCAGCCTC TTCCTCAACG 901 ACCAGGTTAC CCTTCTCAAG TATGGCGTGC ACGAGGCCAT CTTCGCCATG CTGGCCTCTA 961 TCGTCAACAA GGACGGGCTG CTGGTAGCCA ACGGCAGTGG CTTTGTCACC CGTGAGTTCC 1021 TGCGCAGCCT CCGCAAACCC TTCAGTGATA TCATTGAGCC TAAGTTTGAA TTTGCTGTCA 1081 AGTTCAACGC CCTGGAACTT GATGACAGTG ACCTGGCCCT ATTCATTGCG GCCATCATTC 1141 TGTGTGGAGG TGAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1201 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1261 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATGCTCAACA GCCAACGAAC 1321 TGGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt