Construct: ORF TRCN0000488445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019530.1_s317c1
- DNA Barcode:
- GATCACTGAGCTGGTAGGCGGGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPK14 (1432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488445
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_001315.2 | 99.4% | 70.9% | 760G>C;762_763insAGTCT |
| 2 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139012.2 | 96.4% | 67.5% | (many diffs) |
| 3 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010300.2 | 85% | 77.8% | (many diffs) |
| 4 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139014.2 | 82.3% | 76.8% | (many diffs) |
| 5 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010299.2 | 82.1% | 74.1% | (many diffs) |
| 6 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_011514310.3 | 80.9% | 53.2% | (many diffs) |
| 7 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139013.3 | 77.9% | 81.8% | (many diffs) |
| 8 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010301.2 | 76.4% | 80.7% | (many diffs) |
| 9 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_006714998.3 | 75.2% | 46.2% | (many diffs) |
| 10 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010302.2 | 71% | 85.4% | (many diffs) |
| 11 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010303.2 | 70.9% | 86.7% | (many diffs) |
| 12 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010304.2 | 66.3% | 91.2% | (many diffs) |
| 13 | human | 1432 | MAPK14 | mitogen-activated protein k... | XR_926065.2 | 54.7% | (many diffs) | |
| 14 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168508.1 | 89.4% | 70.6% | (many diffs) |
| 15 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_011951.3 | 86.8% | 67.3% | (many diffs) |
| 16 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | XM_017317480.1 | 70.8% | 49.5% | (many diffs) |
| 17 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168513.1 | 68.3% | 46.2% | (many diffs) |
| 18 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168514.1 | 68.3% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 882
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tcccgctgga aaatgtctca ggagaggccc acgttctacc 121 ggcaggagct gaacaagaca atctgggagg tgcccgagcg ttaccagaac ctgtctccag 181 tgggctctgg cgcctatggc tctgtgtgtg ctgcttttga cacaaaaacg gggttacgtg 241 tggcagtgaa gaagctctcc agaccatttc agtccatcat tcatgcgaaa agaacctaca 301 gagaactgcg gttacttaaa catatgaaac atgaaaatgt gattggtctg ttggacgttt 361 ttacacctgc aaggtctctg gaggaattca atgatgtgta tctggtgacc catctcatgg 421 gggcagatct gaacaacatt gtgaaatgtc agaagcttac agatgaccat gttcagttcc 481 ttatctacca aattctccga ggtctaaagt atatacattc agctgacata attcacaggg 541 acctaaaacc tagtaatcta gctgtgaatg aagactgtga gctgaagatt ctggattttg 601 gactggctcg gcacacagat gatgaaatga caggctacgt ggccactagg tggtacaggg 661 ctcctgagat catgctgaac tggatgcatt acaaccagac agttgatatt tggtcagtgg 721 gatgcataat ggccgagctg ttgactggaa gaacattgtt tcctggtaca gaccatatta 781 accagcttca gcagattatg cgtctgacag gaacaccccc cgcttatctc attaacagga 841 tgccaagcca TCAGAGTCTG CAAGAAACTA TATTCAGTCT TTGACTCAGA TGCCGAAGAT 901 GAACTTTGCG AATGTATTTA TTGGTGCCAA TCCCCTGGCT GTCGACTTGC TGGAGAAGAT 961 GCTTGTATTG GACTCAGATA AGAGAATTAC AGCGGCCCAA GCCCTTGCAC ATGCCTACTT 1021 TGCTCAGTAC CACGATCCTG ATGATGAACC AGTGGCCGAT CCTTATGATC AGTCCTTTGA 1081 AAGCAGGGAC CTCCTTATAG ATGAGTGGAA AAGCCTGACC TATGATGAAG TCATCAGCTT 1141 TGTGCCACCA CCCCTTGACC AAGAAGAGAT GGAGTCCAAG GGTGGGCGCG CCGACCCAGC 1201 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 1261 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 1321 ATCTTGTGGA AAGGACGAGA TCACTGAGCT GGTAGGCGGG CAACGCGTTA AGTCgacaat 1381 caacctctgg attacaaaat ttgtgaaaga tt