Construct: ORF TRCN0000488462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020161.1_s317c1
- DNA Barcode:
- TTCAGTCTCGCGCTAGGCGCCATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K7 (6885)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488462
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_003188.4 | 100% | 100% | |
| 2 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_145331.3 | 95.5% | 95.5% | 1208_1288del |
| 3 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_145333.3 | 84.8% | 82.9% | 1443_1444ins116;1473_1474ins148 |
| 4 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_145332.3 | 81% | 79.3% | 1208_1288del;1524_1525ins116;1554_1555ins148 |
| 5 | human | 6885 | MAP3K7 | mitogen-activated protein k... | XM_017011226.2 | 77.5% | 77.5% | 0_1ins390 |
| 6 | human | 6885 | MAP3K7 | mitogen-activated protein k... | XM_006715553.3 | 74% | 74% | 0_1ins390;818_898del |
| 7 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | NM_172688.3 | 91.7% | 99.3% | (many diffs) |
| 8 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | NM_009316.1 | 87.8% | 94.8% | (many diffs) |
| 9 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | XM_006537948.3 | 68.5% | 73.5% | (many diffs) |
| 10 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | XR_390323.3 | 24.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1809
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctaca gcctctgccg cctcctcctc ctcctcgtct tcggccggtg 121 agatgatcga agccccttcc caggtcctca actttgaaga gatcgactac aaggagatcg 181 aggtggaaga ggttgttgga agaggagcct ttggagttgt ttgcaaagct aagtggagag 241 caaaagatgt tgctattaaa caaatagaaa gtgaatctga gaggaaagcg tttattgtag 301 agcttcggca gttatcccgt gtgaaccatc ctaatattgt aaagctttat ggagcctgct 361 tgaatccagt gtgtcttgtg atggaatatg ctgaaggggg ctctttatat aatgtgctgc 421 atggtgctga accattgcca tattatactg ctgcccacgc aatgagttgg tgtttacagt 481 gttcccaagg agtggcttat cttcacagca tgcaacccaa agcgctaatt cacagggacc 541 tgaaaccacc aaacttactg ctggttgcag gggggacagt tctaaaaatt tgtgattttg 601 gtacagcctg tgacattcag acacacatga ccaataacaa ggggagtgct gcttggatgg 661 cacctgaagt ttttgaaggt agtaattaca gtgaaaaatg tgacgtcttc agctggggta 721 ttattctttg ggaagtgata acgcgtcgga aaccctttga tgagattggt ggcccagctt 781 tccgaatcat gtgggctgtt cataatggta ctcgaccacc actgataaaa aatttaccta 841 agcccattga gagcctgatg actcgttgtt ggtctaaaga tccttcccag cgcccttcaa 901 tggaggaaat tgtgaaaata atgactcact tgatgcggta ctttccagga gcagatgagc 961 cattacagta tccttgtcag tattcagatg aaggacagag caactctgcc accagtacag 1021 gctcattcat ggacattgct tctacaaata cgagtaacaa aagtgacact aatatggagc 1081 aagttcctgc cacaaatgat actattaagc gcttagaatc aaaattgttg aaaaatcagg 1141 caaagcaaca gagtgaatct ggacgtttaa gcttgggagc ctcccgtggg agcagtgtgg 1201 agagcttgcc cccaacctct gagggcaaga ggatgagtgc tgacatgtct gaaatagaag 1261 ctaggatcgc cgcaaccaca ggcaacggac agccaagacg tagatccatc caagacttga 1321 ctgtaactgg aacagaacct ggtcaggtga gcagtaggtc atccagtccc agtgtcagaa 1381 tgattactac ctcaggacca acctcagaaa agccaactcg aagtcatcca tggacccctg 1441 atgattccac agataccaat ggatcagata actccatccc aatggcttat cttacactGG 1501 ATCACCAACT ACAGCCTCTA GCACCGTGCC CAAACTCCAA AGAATCTATG GCAGTGTTTG 1561 AACAGCATTG TAAAATGGCA CAAGAATATA TGAAAGTTCA AACAGAAATT GCATTGTTAT 1621 TACAGAGAAA GCAAGAACTA GTTGCAGAAC TGGACCAGGA TGAAAAGGAC CAGCAAAATA 1681 CATCTCGCCT GGTACAGGAA CATAAAAAGC TTTTAGATGA AAACAAAAGC CTTTCTACTT 1741 ACTACCAGCA ATGCAAAAAA CAACTAGAGG TCATCAGAAG TCAGCAGCAG AAACGACAAG 1801 GCACTTCATG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1861 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1921 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTC AGTCTCGCGC TAGGCGCCAT 1981 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t