Construct: ORF TRCN0000488494

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021604.1_s317c1
DNA Barcode:
TCCCTACTTCATCTTTCGAAAGCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PPARD (5467)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488494
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5467 PPARD peroxisome proliferator act... NM_177435.3 99.9% 100% 489C>T
2 human 5467 PPARD peroxisome proliferator act... XM_024446474.1 98.7% 98.3% (many diffs)
3 human 5467 PPARD peroxisome proliferator act... NM_001171818.2 81.6% 81.4% (many diffs)
4 human 5467 PPARD peroxisome proliferator act... NM_006238.5 81.6% 81.4% (many diffs)
5 human 5467 PPARD peroxisome proliferator act... XM_005249193.1 81.6% 81.4% (many diffs)
6 human 5467 PPARD peroxisome proliferator act... XM_006715120.1 81.6% 81.4% (many diffs)
7 human 5467 PPARD peroxisome proliferator act... XM_006715123.1 81.6% 81.4% (many diffs)
8 human 5467 PPARD peroxisome proliferator act... XM_011514707.1 81.6% 81.4% (many diffs)
9 human 5467 PPARD peroxisome proliferator act... XM_011514710.1 81.6% 81.4% (many diffs)
10 human 5467 PPARD peroxisome proliferator act... XM_017010972.1 81.6% 81.4% (many diffs)
11 human 5467 PPARD peroxisome proliferator act... XM_017010973.1 81.6% 81.4% (many diffs)
12 human 5467 PPARD peroxisome proliferator act... XM_017010974.1 81.6% 81.4% (many diffs)
13 human 5467 PPARD peroxisome proliferator act... NM_001171819.1 72.6% 71.6% (many diffs)
14 human 5467 PPARD peroxisome proliferator act... NM_001171820.1 59.4% 59.1% (many diffs)
15 mouse 19015 Ppard peroxisome proliferator act... NM_011145.3 73% 75.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1155
ORF length:
1083
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggagcag ccacaggagg aagcccctga ggtccgggaa gaggaggaga
121aagaggaagt ggcagaggca gaaggagccc cagagctcaa tgggggacca cagcatgcac
181ttccttccag cagctacaca gacctctccc ggagctcctc gccaccctca ctgctggacc
241aactgcagat gggctgtgac ggggcctcat gcggcagcct caacatggag tgccgggtgt
301gcggggacaa ggcatcgggc ttccactacg gtgttcatgc atgtgagggg tgcaagggct
361tcttccgtcg tacgatccgc atgaagctgg agtacgagaa gtgtgagcgc agctgcaaga
421ttcagaagaa gaaccgcaac aagtgccagt actgccgctt ccagaagtgc ctggcactgg
481gcatgtcaca caacgctatc cgttttggtc ggatgccgga ggctgagaag aggaagctgg
541tggcagggct gactgcaaat gaggggagcc agtacaaccc acaggtggcc gacctgaagg
601ccttctccaa gcacatctac aatgcctacc tgaaaaactt caacatgacc aaaaagaagg
661cccgcagcat cctcaccggc aaagccagcc acacggcgcc ctttgtgatc cacgacatcg
721agacattgtg gcaggcagag aaggggctgg tgtggaagca gttggtgaat ggcctgcctc
781cctacaagga gatcagcgtg cacgtcttct accgctgcca gtgcaccaca gtggagaccg
841tgcgggagct cactgagttc gccaagagca tccccagctt cagcagcctc ttcctcaacg
901accaggttac ccttctcaag tatggcgtgc acgaggccat cTTCGCCATG CTGGCCTCTA
961TCGTCAACAA GGACGGGCTG CTGGTAGCCA ACGGCAGTGG CTTTGTCACC CGTGAGTTCC
1021TGCGCAGCCT CCGCAAACCC TTCAGTGATA TCATTGAGCC TAAGTTTGAA TTTGCTGTCA
1081AGTTCAACGC CCTGGAACTT GATGACAGTG ACCTGGCCCT ATTCATTGCG GCCATCATTC
1141TGTGTGGAGG TGAGTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1201TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1261GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCCCTAC TTCATCTTTC
1321GAAAGCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)