Construct: ORF TRCN0000488546

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019723.1_s317c1
DNA Barcode:
TAATCTTAGTTCATACATGCTTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPEG (10290)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488546
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10290 SPEG striated muscle enriched pr... XM_017003162.2 99.8% 99.4% 510_511insG
2 human 10290 SPEG striated muscle enriched pr... XM_017003161.2 94.4% 93.8% 507delA;510T>A;513_540del
3 human 10290 SPEG striated muscle enriched pr... NM_001173476.2 66.3% 66% 0_1ins171;339_340insG
4 human 10290 SPEG striated muscle enriched pr... XM_024452526.1 66.3% 66% 0_1ins171;339_340insG
5 human 10290 SPEG striated muscle enriched pr... XR_001738587.2 10.6% (many diffs)
6 human 10290 SPEG striated muscle enriched pr... XM_017003159.2 9.2% 9.1% 506_517del;520_520delCinsGA;523_5490del
7 human 10290 SPEG striated muscle enriched pr... XM_005246239.2 6.8% 6.7% 506_517del;520_520delCinsGA;523_7425del
8 human 10290 SPEG striated muscle enriched pr... XM_005246242.4 6.5% 6% (many diffs)
9 human 10290 SPEG striated muscle enriched pr... XM_006712189.3 5.3% 5.3% (many diffs)
10 human 10290 SPEG striated muscle enriched pr... XM_005246237.2 5.3% 5.2% (many diffs)
11 human 10290 SPEG striated muscle enriched pr... XM_017003157.1 5.3% 5.2% (many diffs)
12 human 10290 SPEG striated muscle enriched pr... XM_011510483.2 5.3% 5.2% (many diffs)
13 human 10290 SPEG striated muscle enriched pr... NM_005876.5 5.1% 5.1% (many diffs)
14 human 10290 SPEG striated muscle enriched pr... XM_011510479.2 5.1% 5.1% (many diffs)
15 human 10290 SPEG striated muscle enriched pr... XM_005246240.2 4.5% 4.4% (many diffs)
16 human 10290 SPEG striated muscle enriched pr... XM_005246241.1 4.5% 4.4% (many diffs)
17 human 10290 SPEG striated muscle enriched pr... XM_006712193.3 4.5% 4.4% (many diffs)
18 human 10290 SPEG striated muscle enriched pr... XM_017003158.2 4.5% 4.4% (many diffs)
19 mouse 11790 Speg SPEG complex locus NM_001173477.1 58.1% 62.5% (many diffs)
20 mouse 11790 Speg SPEG complex locus XM_017312276.1 17.5% 18.8% (many diffs)
21 mouse 11790 Speg SPEG complex locus NM_001085371.1 17.4% 18.6% (many diffs)
22 mouse 11790 Speg SPEG complex locus XM_011238651.2 15.3% 16.4% (many diffs)
23 mouse 11790 Speg SPEG complex locus XM_011238650.2 8.5% 9.1% (many diffs)
24 mouse 11790 Speg SPEG complex locus NM_001085370.1 5.9% 6.2% (many diffs)
25 mouse 11790 Speg SPEG complex locus XM_006496400.2 5.9% 6.2% (many diffs)
26 mouse 11790 Speg SPEG complex locus XM_006496401.2 5.9% 6.2% (many diffs)
27 mouse 11790 Speg SPEG complex locus XM_011238648.2 5.9% 6.2% (many diffs)
28 mouse 11790 Speg SPEG complex locus XM_011238649.2 5.9% 6.2% (many diffs)
29 mouse 11790 Speg SPEG complex locus XM_006496402.2 5.7% 5.7% (many diffs)
30 mouse 11790 Speg SPEG complex locus XM_011238647.1 4.8% 5.1% (many diffs)
31 mouse 11790 Speg SPEG complex locus XM_017322375.1 4.7% 5% (many diffs)
32 mouse 11790 Speg SPEG complex locus XM_011238646.1 4.7% 5% (many diffs)
33 mouse 11790 Speg SPEG complex locus XM_011238645.1 4.7% 5% (many diffs)
34 mouse 11790 Speg SPEG complex locus XM_017322354.1 4.6% 4.9% (many diffs)
35 mouse 11790 Speg SPEG complex locus XM_011238644.1 4.6% 4.9% (many diffs)
36 mouse 11790 Speg SPEG complex locus NM_007463.4 4.5% 4.8% (many diffs)
37 mouse 11790 Speg SPEG complex locus XM_011238643.1 4.5% 4.8% (many diffs)
38 mouse 11790 Speg SPEG complex locus XM_006496403.3 4% 4.2% (many diffs)
39 mouse 11790 Speg SPEG complex locus XM_006496404.3 4% 4.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
585
ORF length:
513
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggccacc gccaccaacg agctgggcca ggccacctgt gccgcctcac
121tgaccgtgag acccggtggg tctacatccc ctttcagcag ccccatcacc tccgacgagg
181aatacctgag ccccccagag gagttcccag agcctgggga gacctggccg cgaaccccca
241ccatgaagcc cagtcccagc cagaaccgcc gttcttcTGA CACTGGCTCC AAGGCACCCC
301CCACCTTCAA GGTCTCACTT ATGGACCAGT CAGTAAGAGA AGGCCAAGAT GTCATCATGA
361GCATCCGCGT GCAGGGGGAG CCCAAGCCTG TGGTCTCCTG GCTGAGAAAC CGCCAGCCCG
421TGCGCCCAGA CCAGCGGCGC TTTGCGGAGG AGGCTGAGGG TGGGCTGTGC CGGCTGCGGA
481TCCTGGCTGC AGAGCGTGGC GATGCTGGTT TCTACACTTG CAAAGCGGTC AATGAGTATG
541GTGCTCGGCA GTGCGAGGCC CGCTTGGAGG TCCGAGGCGA GGACCCAGCT TTCTTGTACA
601AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
661AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
721AGGACGATAA TCTTAGTTCA TACATGCTTT CACGCGTTAA GTCgacaatc aacctctgga
781ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)