Construct: ORF TRCN0000488599

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021389.1_s317c1
DNA Barcode:
CCAAATTTATCCGAGAAATGGTGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
SULF2 (55959)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488599
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55959 SULF2 sulfatase 2 NM_001161841.1 99.6% 100% 291T>C;2610_2611insTAAAAGCT
2 human 55959 SULF2 sulfatase 2 NM_018837.3 99.6% 100% 291T>C;2610_2611insTAAAAGCT
3 human 55959 SULF2 sulfatase 2 XM_005260458.3 99.6% 100% 291T>C;2610_2611insTAAAAGCT
4 human 55959 SULF2 sulfatase 2 XM_017027955.2 99.6% 100% 291T>C;2610_2611insTAAAAGCT
5 human 55959 SULF2 sulfatase 2 XM_006723830.2 99.6% 100% 291T>C;1773T>C;2610_2611insTAAAAGCT
6 human 55959 SULF2 sulfatase 2 XM_006723827.2 99.5% 99.8% 291T>C;1805_1807delGCA;2613_2614insTAAAAGCT
7 human 55959 SULF2 sulfatase 2 XM_006723828.2 99.5% 99.8% 291T>C;1805_1807delGCA;2613_2614insTAAAAGCT
8 human 55959 SULF2 sulfatase 2 XM_006723829.3 99.5% 99.8% 291T>C;1805_1807delGCA;2613_2614insTAAAAGCT
9 human 55959 SULF2 sulfatase 2 XM_011528913.1 99.5% 99.8% 291T>C;1805_1807delGCA;2613_2614insTAAAAGCT
10 human 55959 SULF2 sulfatase 2 XM_011528914.2 99.5% 99.8% (many diffs)
11 human 55959 SULF2 sulfatase 2 NM_198596.2 99.3% 99.6% 291T>C;2493_2494insGGACTTAAA;2601_2602insTAAAAGCT
12 human 55959 SULF2 sulfatase 2 XM_011528916.2 97.5% 97.9% 291T>C;2528_2529ins54;2556_2557insTAAAAGCT
13 human 55959 SULF2 sulfatase 2 XM_011528915.2 97.4% 97.8% (many diffs)
14 mouse 72043 Sulf2 sulfatase 2 NM_001252578.1 87.1% 93.7% (many diffs)
15 mouse 72043 Sulf2 sulfatase 2 NM_028072.5 87.1% 93.7% (many diffs)
16 mouse 72043 Sulf2 sulfatase 2 XM_006500209.3 87.1% 93.7% (many diffs)
17 mouse 72043 Sulf2 sulfatase 2 XM_006500211.2 87.1% 93.7% (many diffs)
18 mouse 72043 Sulf2 sulfatase 2 XM_017319268.1 87.1% 93.7% (many diffs)
19 mouse 72043 Sulf2 sulfatase 2 NM_001252579.1 83.9% 90.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2676
ORF length:
2610
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat
61tgaccatggg ccccccgagc ctcgtgctgt gcttgctgtc cgcaactgtg ttctccctgc
121tgggtggaag ctcggccttc ctgtcgcacc accgcctgaa aggcaggttt cagagggacc
181gcaggaacat ccgccccaac atcatcctgg tgctgacgga cgaccaggat gtggagctgg
241gttccatgca ggtgatgaac aagacccggc gcatcatgga gcagggcggg gcgcacttca
301tcaacgcctt cgtgaccaca cccatgtgct gcccctcacg ctcctccatc ctcaccggca
361agtacgtcca caaccacaac acctacacca acaatgagaa ctgctcctcg ccctcctggc
421aggcacagca cgagagccgc acctttgccg tgtacctcaa tagcactggc taccggacag
481ctttcttcgg gaagtatctt aatgaataca acggctccta cgtgccaccc ggctggaagg
541agtgggtcgg actccttaaa aactcccgct tttataacta cacgctgtgt cggaacgggg
601tgaaagagaa gcacggctcc gactactcca aggattacct cacagacctc atcaccaatg
661acagcgtgag cttcttccgc acgtccaaga agatgtaccc gcacaggcca gtcctcatgg
721tcatcagcca tgcagccccc cacggccctg aggattcagc cccacaatat tcacgcctct
781tcccaaacgc atctcagcac atcacgccga gctacaacta cgcgcccaac ccggacaaac
841actggatcat gcgctacacg gggcccatga agcccatcca catggaattc accaacatgc
901tccagcggaa gcgcttgcag accctcatgt cggtggacga ctccatggag acgatttaca
961acatgctggt tgagacgggc gagctggaca acacgtacat cgtatacacc gccgaccacg
1021gttaccacat cggccagttt ggcctggtga aagggaaatc catgccatat gagtttgaca
1081tcagggtccc gttctacgtg aggggcccca acgtggaagc cggctgtctg aatccccaca
1141tcgtcctcaa cattgacctg gcccccacca tcctggacat tgcaggcctg gacatacctg
1201cggatatgga cgggaaatcc atcctcaagc tgctggacac ggagcggccg gtgaatcggt
1261ttcacttgaa aaagaagatg agggtctggc gggactcctt cttggtggag agaggcaagc
1321tgctacacaa gagagacaat gacaaggtgg acgcccagga ggagaacttt ctgcccaagt
1381accagcgtgt gaaggacctg tgtcagcgtg ctgagtacca gacggcgtgt gagcagctgg
1441gacagaagtg gcagtgtgtg gaggacgcca cggggaagct gaagctgcat aagtgcaagg
1501gccccatgcg gctgggcggc agcagagccc tctccaacct cgtgcccaag tactacgggc
1561agggcagcga ggcctgcacc tgtgacagcg gggactacaa gctcagcctg gccggacgcc
1621ggaaaaaact cttcaagaag aagtacaagg ccagctatgt ccgcagtcgc tccatccgct
1681cagtggccat cgaggtggac ggcagggtgt accacgtagg cctgggtgat gccgcccagc
1741cccgaaacct caccaagcgg cactggccag gggcccctga ggaccaagat gacaaggatg
1801gtggggactt cagtggcact ggaggccttc ccgactactc agccgccaac cccattaaag
1861tgacacatcg gtgctacatc ctagagaacg acacagtcca gtgtgacctg gacctgtaca
1921agtccctgca ggcctggaaa gaccacaagc tgcacatcga ccacgagatt gaaaccctgc
1981agaacaaaat taagaacctg agggaagtcc gaggtcacct gaagaaaaag cggccagaag
2041aatgtgactg tcacaaaatc agctaccaca cccagcacaa aggccgcctc aagcacagag
2101gctccagtct gcatcctttc aggaagggcc tgcaagagaa ggacaaggtg tggctgttgc
2161gggagcagaa gcgcaagaag aaactccgca agctgctcaa gcgcctgcag aacaacgaca
2221cgtgcagcat gccaggcctc acgtgcttca cccacgacaa ccagcactgg cagacggcgc
2281ctttctggac actggggcct ttctgtgcct gcaccagcgc caacaataac acgtactggt
2341gcatgaggac catcaatgag actcacaatt tcctcTTCTG TGAATTTGCA ACTGGCTTCC
2401TAGAGTACTT TGATCTCAAC ACAGACCCCT ACCAGCTGAT GAATGCAGTG AACACACTGG
2461ACAGGGATGT CCTCAACCAG CTACACGTAC AGCTCATGGA GCTGAGGAGC TGCAAGGGTT
2521ACAAGCAGTG TAACCCCCGG ACTCGAAACA TGGACCTGGG ACTTAAAGAT GGAGGAAGCT
2581ATGAGCAATA CAGGCAGTTT CAGCGTCGAA AGTGGCCAGA AATGAAGAGA CCTTCTTCCA
2641AATCACTGGG ACAACTGTGG GAAGGCTGGG AAGGTTAAAA GCTTGACCCA GCTTTCTTGT
2701ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
2761AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
2821GAAAGGACGA CCAAATTTAT CCGAGAAATG GTGTACGCGT TAAGTCgaca atcaacctct
2881ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)