Construct: ORF TRCN0000488605

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020460.1_s317c1
DNA Barcode:
TATCTCCTCTGCAGGTCTTGTGGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
AK5 (26289)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488605
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26289 AK5 adenylate kinase 5 NM_174858.3 100% 100%
2 human 26289 AK5 adenylate kinase 5 XM_005270739.5 95.7% 95.7% 246_247ins72
3 human 26289 AK5 adenylate kinase 5 NM_012093.4 95.3% 95.3% 0_1ins78
4 human 26289 AK5 adenylate kinase 5 XM_006710572.3 95.3% 95.3% 0_1ins78
5 human 26289 AK5 adenylate kinase 5 XM_017001008.2 95.3% 95.3% 0_1ins78
6 human 26289 AK5 adenylate kinase 5 XM_017001009.1 95.3% 95.3% 0_1ins78
7 human 26289 AK5 adenylate kinase 5 XM_017001010.2 91.1% 91.1% 0_1ins78;168_169ins72
8 human 26289 AK5 adenylate kinase 5 XM_017001011.2 91.1% 91.1% 0_1ins78;168_169ins72
9 human 26289 AK5 adenylate kinase 5 XM_017001012.1 68.8% 68.6% (many diffs)
10 mouse 229949 Ak5 adenylate kinase 5 NM_001081277.1 86.4% 91.4% (many diffs)
11 mouse 229949 Ak5 adenylate kinase 5 XM_011240115.2 84.4% 87.5% (many diffs)
12 mouse 229949 Ak5 adenylate kinase 5 XM_006501440.3 83% 88% (many diffs)
13 mouse 229949 Ak5 adenylate kinase 5 XM_006501441.3 54.8% 59.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1758
ORF length:
1686
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaacacc aacgatgcca aggagtatct ggcccggagg gaaatccctc
121agctttttga gagccttttg aatggactga tgtgttctaa gcccgaagat ccagtagaat
181acttggaaag ttgtttacaa aaagtaaagg aactgggtgg ctgtgacaag gtgaaatggg
241atacatttgt aagccaggaa aagaagacct tacctccact aaatggagga cagtcacgga
301gatcctttct aagaaatgta atgcctgaaa actcaaactt tccatatcgg cggtatgacc
361ggctccctcc aatccatcaa ttctccatag aaagtgacac ggatctctct gagactgcag
421agttgattga ggagtatgag gtttttgatc ctaccagacc tcgaccaaaa atcattcttg
481ttataggtgg tccaggaagt ggaaagggta ctcagagttt gaaaattgca gaacgatatg
541gattccaata catttctgtg ggagaattat taagaaagaa gatccacagt accagcagca
601ataggaaatg gagtcttatt gccaagataa ttacaactgg agaattggcc ccacaggaaa
661caacaattac agagataaaa caaaaattga tgcaaatacc tgatgaagag ggcattgtta
721ttgatggatt tccaagagat gttgcccagg ctctatcttt tgaggaccaa atctgtaccc
781ccgatttggt ggtattcctg gcttgtgcta atcagagact caaagaaaga ttactgaagc
841gtgcagaaca gcagggccga ccagacgaca atgtaaaagc tacccaaagg agactaatga
901acttcaagca gaatgctgct ccattggtta aatacttcca ggaaaagggg ctcatcatga
961catttgatgc cgaccgcgat gaggatgagg tgttctatga catcagcatg gcagttgaca
1021acaagttatt tccaaacaaa gaggctgcag caggttcaag tgaccttgat ccttcgatga
1081tattggacac tggagagatc attgatacag gatctgatta tgaagatcag ggtgatgacc
1141agttaaatgt atttggagag gacactatgg gaggtttcat ggaagatttg agaaagtgta
1201aaattatttt cataattggt ggtcctggct ctggcaaagg cacacagtgt gaaaagctgg
1261tggaaaaata tggatttaca catctctcaa ctggcgagct cctgcgtgag gaactggcat
1321cagaatctga aagaagcaaa ttgatcagag acattatgga acgtggagac ctggtgccct
1381caggcatcgt tttggagctc ctgaaggagg ccatggtggc cagcctcggg gacaccaggg
1441gcttccTGAT TGACGGCTAT CCTCGGGAGG TGAAGCAAGG GGAAGAGTTC GGACGCAGGA
1501TTGGAGACCC ACAGTTGGTG ATCTGTATGG ACTGCTCGGC AGACACCATG ACCAACCGCC
1561TTCTCCAAAG GAGCCGGAGC AGCCTGCCTG TGGACGACAC CACCAAGACC ATCGCCAAGC
1621GCCTAGAAGC CTACTACCGA GCGTCCATCC CCGTGATCGC CTACTACGAG ACAAAAACAC
1681AGCTACACAA GATAAATGCA GAGGGAACAC CAGAGGACGT TTTTCTTCAA CTCTGCACAG
1741CTATTGACTC TATTTTCTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1801CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1861AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATATC TCCTCTGCAG
1921GTCTTGTGGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)