Construct: ORF TRCN0000488612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019300.1_s317c1
- DNA Barcode:
- GTGGATCTCCCCGGTCACGTTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SORCS3 (22986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488612
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22986 | SORCS3 | sortilin related VPS10 doma... | NM_014978.3 | 99.9% | 100% | 1368C>T |
| 2 | human | 22986 | SORCS3 | sortilin related VPS10 doma... | XM_011539542.2 | 70.8% | 70.8% | 0_1ins1068;300C>T |
| 3 | mouse | 66673 | Sorcs3 | sortilin-related VPS10 doma... | NM_025696.3 | 88.2% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3741
- ORF length:
- 3666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag gcggcgcgca cggagcgccc cgcaggcagg ccgggggcgc 121 cgcttgtccg gacggggctc ctactcttgt cgacgtgggt cctggccggc gccgagatca 181 cttgggacgc gacaggcggt cccggacgcc cggcggcccc ggcttcgcgg ccaccggcgt 241 tgtctccact ctcgccgcgg gcagtggcca gccagtggcc ggaggagctg gcgtcggcgc 301 ggagagccgc cgtgctgggg cgccgggccg gaccagagct gctgccccag cagggcggcg 361 gcagaggcgg tgagatgcag gtggaagccg gagggacatc accggcaggc gagcggcggg 421 gccggggcat cccagctcct gccaagcttg gcggcgcgag gaggagtcgc cgggcgcagc 481 ccccaatcac ccaggaacgc ggggacgcct gggccactgc tccggccgat ggttccagag 541 gaagccgtcc ccttgctaag ggttcccggg aggaggtgaa ggcgccgcgg gctggggggt 601 cggcggctga agacctccgg ctgcccagca cctccttcgc gctgaccggg gactcggccc 661 acaaccaagc catggtgcac tggtcgggac acaacagcag cgtcatactt atcctgacga 721 agctgtatga cttcaacctg ggcagcgtga ctgagagttc actatggagg tcgacagatt 781 atggcaccac ctatgaaaag ctgaatgaca aagtgggttt gaagactgtc ctcagttacc 841 tctatgtcaa tccaaccaac aaaaggaaga ttatgcttct cagtgatcct gagatggaga 901 gcagcatatt gatcagctca gacgaagggg cgacctatca gaagtatcgg ctcaccttct 961 atatccagag cctgctcttt catcccaagc aagaggactg ggtgctggcc tacagtttgg 1021 atcaaaagct ctacagctcc atggactttg gaagacggtg gcaactcatg catgaacgca 1081 tcacacccaa caggttttat tggtcggtgg ccggattgga taaggaggcg gacctggtgc 1141 acatggaggt gcggaccacg gatggatatg ctcactacct cacctgcagg atccaggaat 1201 gtgccgagac aactagaagt gggccttttg cccgctccat tgacatcagt tccctggttg 1261 tccaggatga atatatcttc attcaggtaa caactagtgg aagagccagc tactacgtgt 1321 cttatcgaag agaggccttt gctcagataa agctgcctaa gtactcgttg ccaaaggaca 1381 tgcacatcat cagtacagac gagaaccaag tatttgctgc ggtccaagaa tggaaccaga 1441 atgacacgta caacctctac atctcagaca cgcgtgggat ttacttcact ctggccatgg 1501 agaacatcaa gagcagcaga ggtctaatgg ggaacatcat tattgaattg tatgaggtag 1561 caggtatcaa agggatattt ctggcaaaca agaaggtgga cgaccaggtg aagacataca 1621 tcacttacaa caaaggcagg gattggcgcc tgctgcaagc tccggatgtg gacctgagag 1681 gaagcccagt gcactgcctg ctgcccttct gttccttaca tctgcacctg caactctctg 1741 aaaatccata ttcctcagga agaatctcta gcaaggagac agccccagga cttgtggtgg 1801 ctacaggcaa cattggcccg gagctctcat atactgatat tggtgtgttc atctcctccg 1861 atgggggcaa cacatggaga cagatctttg atgaagagta caatgtctgg ttcctagact 1921 ggggtggtgc cctcgtggcc atgaaacaca cacctctgcc agtcaggcat ttgtgggtga 1981 gttttgatga gggccactct tgggacaagt atggtttcac ttcggttcct ctctttgttg 2041 acggggctct ggtggaggca ggaatggaga cccacatcat gacagttttt ggccacttca 2101 gcctccgctc cgaatggcaa ttggtgaaag tggactacaa atctatcttc agccggcatt 2161 gcaccaagga ggactatcag acctggcacc tgctcaatca gggagagcct tgtgtcatgg 2221 gagaaaggaa aatattcaag aaacgtaagc caggagctca gtgtgccctg ggccgagacc 2281 actcaggatc agtggtctca gaaccctgtg tctgtgccaa ttgggacttc gagtgtgact 2341 atgggtatga gagacatggg gagagccagt gtgtcccagc tttctggtac aatccagcat 2401 ccccatcaaa ggactgcagc cttggtcaaa gctaccttaa cagcactggg tatcggcgga 2461 ttgtgtccaa caactgcaca gatgggctaa gggagaagta caccgccaag gcccagatgt 2521 gccctggaaa agcccctcgg ggcctccatg tggtgacgac cgatgggcgg ctggtggcag 2581 agcaggggca caatgcaact ttcatcatcc tcatggagga gggtgatcta caaaggacaa 2641 acatccagct tgactttggg gatgggattg ctgtgtccta cgcaaacttc agccccatcg 2701 aggacggcat caagcacgtg tataagagtg cggggatctt ccaggtgaca gcctatgcag 2761 agaacaacct tggctcagac acagctgtcc tcttcctgca tgtggtttgt cctgtggagc 2821 atgttcatct ccgagttcca tttgttgcca taagaaataa ggaggtcaac atcagtgcag 2881 tcgtgtggcc cagtcaactg gggaccctta cctatttctg gtggttcggc aatagcacaa 2941 agcctctcat cactttggac agcagcattt ccttcacatt ccttgcagaa ggaaccgaca 3001 ccatcacagt ccaggtggct gctgggaatg ccctcatcca ggacacaaaa gagattgcag 3061 ttcatgaata tttccagtcc cagcttttat cattctctcc taatctggat taccacaatc 3121 ctgacattcc tgagtggaga aaagatattg gcaatgtcat caagcgagct ctggttaaag 3181 taaccagtgt cccagaggac cagatcctca ttgccgtgtt tcctggtctc cccacttcag 3241 cagagctttt cattcttcca cccaagaacc tgacagagag gaggaaaggc aatgaagggg 3301 acctggaaca aattgtagaa acactgttta atgctctcaa ccaaaatttg gtccagtttg 3361 agctgaagcc gggggtacaa gtcattgtgt atgtcacaca gctgacgtta gctccattgg 3421 tggactccag tgctgggcac agcagcTCAG CCATGCTTAT GCTATTATCA GTGGTATTTG 3481 TTGGCCTGGC TGTGTTTTTG ATCTACAAGT TTAAAAGGAA AATCCCTTGG ATTAACATCT 3541 ATGCTCAAGT CCAACACGAC AAGGAGCAGG AGATGATTGG GTCAGTGAGC CAAAGTGAAA 3601 ACGCCCCCAA AATCACACTC AGTGACTTTA CGGAGCCTGA GGAGCTGCTG GACAAAGAGC 3661 TGGACACGCG GGTCATAGGA GGCATTGCCA CTATTGCAAA CAGCGAAAGC ACAAAGGAGA 3721 TCCCCAACTG CACTAGTGTT GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3781 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3841 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTGG ATCTCCCCGG 3901 TCACGTTGGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt