Construct: ORF TRCN0000488774
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020635.1_s317c1
- DNA Barcode:
- ATCGAATTATCCTTTTGCTGGTCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TNK2 (10188)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488774
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | NM_005781.4 | 100% | 100% | |
| 2 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_005269270.3 | 98.3% | 98.3% | 1543_1587del;2937_2942delGCTGCA |
| 3 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_011512320.1 | 98.3% | 98.3% | 1543_1587del;2937_2942delGCTGCA |
| 4 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_024453293.1 | 98.3% | 98.3% | 1543_1587del;2937_2942delGCTGCA |
| 5 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_024453294.1 | 98.3% | 98.3% | 1543_1587del;2937_2942delGCTGCA |
| 6 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_024453295.1 | 98.3% | 98.3% | 1543_1587del;2937_2942delGCTGCA |
| 7 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_017005509.1 | 96.9% | 96.9% | 1_96del;3207_3208insCGC |
| 8 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_017005508.1 | 96.8% | 96.8% | 1_96del;2988_2993delGCTGCA |
| 9 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_024453292.1 | 96.8% | 96.8% | 1_51del;1594_1638del;2988_2993delGCTGCA |
| 10 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_011512318.2 | 95.6% | 95.6% | 1_96del;1639_1683del |
| 11 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | NM_001308046.1 | 94.2% | 94.2% | 1_96del;2987_2988ins90 |
| 12 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | NM_001010938.2 | 93.5% | 93.5% | 1_72del;1615_1659del;3008_3009ins90 |
| 13 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_017005510.1 | 92.9% | 92.9% | 1_96del;1639_1683del;3032_3033ins90 |
| 14 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_005269268.3 | 92.8% | 92.8% | 1_189del;1732_1776del;3126_3131delGCTGCA |
| 15 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_024453291.1 | 92.7% | 92.7% | 1_192del;1735_1779del;3129_3134delGCTGCA |
| 16 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_011512321.2 | 91.1% | 90.9% | (many diffs) |
| 17 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_011512317.3 | 85.1% | 85.1% | 1_492del;2035_2079del;3429_3434delGCTGCA |
| 18 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_005269274.3 | 75.7% | 75.7% | 0_1ins717;826_870del;2220_2225delGCTGCA |
| 19 | human | 10188 | TNK2 | tyrosine kinase non receptor 2 | XM_005269275.3 | 68.6% | 68.6% | 0_1ins942;601_645del;1995_2000delGCTGCA |
| 20 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_017317066.1 | 87.5% | 94.4% | (many diffs) |
| 21 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | NM_016788.3 | 86.3% | 93% | (many diffs) |
| 22 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522337.3 | 85% | 91.6% | (many diffs) |
| 23 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | NM_001110147.1 | 84.9% | 91.9% | (many diffs) |
| 24 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522336.3 | 84.9% | 91.6% | (many diffs) |
| 25 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_017317065.1 | 84.8% | 91.5% | (many diffs) |
| 26 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522335.1 | 84.4% | 91% | (many diffs) |
| 27 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522334.3 | 83.9% | 90.5% | (many diffs) |
| 28 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522332.3 | 83.7% | 90.3% | (many diffs) |
| 29 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522333.3 | 83.7% | 90.2% | (many diffs) |
| 30 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522340.3 | 82.3% | 89% | (many diffs) |
| 31 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522339.1 | 81.8% | 88.4% | (many diffs) |
| 32 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522338.3 | 81.2% | 87.9% | (many diffs) |
| 33 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_011245946.1 | 72% | 78% | (many diffs) |
| 34 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522342.3 | 51.4% | 54.7% | (many diffs) |
| 35 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | NM_001347185.1 | 45.4% | 48.5% | (many diffs) |
| 36 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522343.1 | 44.7% | 47.8% | (many diffs) |
| 37 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | XM_006522344.1 | 44.7% | 47.8% | (many diffs) |
| 38 | mouse | 51789 | Tnk2 | tyrosine kinase, non-recept... | NM_001289443.1 | 42.7% | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3186
- ORF length:
- 3114
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcagcca gaggagggca caggctggct gctggagctg ctgtccgagg 121 tgcagctgca acagtacttc ctgcggctcc gagatgacct caacgtcacc cgcctgtccc 181 actttgagta cgtcaagaat gaggacctgg agaagatcgg catgggtcgg cctggccagc 241 ggcggctgtg ggaggctgtg aagaggagga aggccttgtg caaacgcaag tcgtggatga 301 gtaaggtgtt cagtggaaag cgactggagg ctgagttccc acctcatcac tctcagagca 361 ccttccggaa gacctcgccc gcccctgggg gcccagcagg ggaggggccc ctgcagagcc 421 tcacctgcct cattggggag aaggacctgc gcctcctgga gaagctgggt gatggttcct 481 ttggcgtggt gcgcaggggc gagtgggacg cgccctcagg gaagacggtg agtgtggctg 541 tgaagtgcct gaagcccgat gtcctgagcc agccagaagc catggacgac ttcatccggg 601 aggtcaatgc catgcactcg ctcgaccacc gaaacctcat ccgcctctac ggggtggtgc 661 tcacgccgcc catgaagatg gtgacagagc tggcacctct gggatcgttg ttggaccggc 721 tacgtaagca ccagggccac ttcctcctgg ggactctgag ccgctacgct gtgcaggtgg 781 ctgagggcat gggctacctg gagtccaagc gctttattca ccgtgacctg gctgcccgca 841 atctgctgtt ggctacccgc gacctggtca agatcgggga ctttgggctg atgcgagcac 901 tacctcagaa tgacgaccat tacgtcatgc aggaacatcg caaggtgccc ttcgcctggt 961 gtgcccccga gagcctgaag acacgcacct tctcccatgc cagcgacacc tggatgttcg 1021 gggtgacact gtgggaaatg ttcacctacg gccaggagcc ctggatcggc ctcaacggca 1081 gtcagatcct gcataagatc gacaaggagg gggagcggct gccccggccc gaggactgtc 1141 cccaggacat ctacaacgtc atggtccagt gctgggctca caagccagag gacagaccca 1201 cgtttgtggc cctgcgggac ttcctgctgg aggcccagcc cacagacatg cgggcccttc 1261 aggactttga ggaaccggac aagctgcaca tccagatgaa tgatgtcatc accgtcatcg 1321 agggaagggc cgagaactac tggtggcgtg gccagaacac acggacgctg tgtgtggggc 1381 ccttccctcg caacgtggtg acctccgtgg ccggcctgtc ggcccaggac atcagccagc 1441 ccctgcagaa cagcttcatc cacacagggc atggcgacag tgacccccgc cactgctggg 1501 gcttcccgga caggattgac gaactgtatc tgggaaaccc catggacccc cccgacctcc 1561 tgagcgtgga actgagcacc tcccggcccc cccagcatct aggaggggtg aaaaaaccaa 1621 cctatgaccc tgtgagcgag gaccaagacc ccttgtccag cgacttcaag aggctgggcc 1681 tgcggaagcc aggcctgccc cgagggctgt ggctggcgaa gccctcggcg cgggtgccgg 1741 gcaccaaggc cagccgaggc agcggggctg aggtcacgct catcgacttc ggtgaggagc 1801 ccgtggtccc ggccctacgg ccctgcgcgc cctccctggc gcagctggcc atggacgcct 1861 gctccctgct ggacgagacc ccgcctcaga gccccacgcg ggcactgccc cggcccctgc 1921 accccacgcc tgtggtggac tgggacgcac gcccgctgcc ccccccgccc gcctatgacg 1981 acgtggccca ggatgaggat gactttgaga tctgctccat caacagcacc ctcgtgggcg 2041 cgggggtccc tgccgggccc agccagggcc agaccaacta cgcctttgtg cctgagcagg 2101 cgcggccgcc ccctcccctg gaggacaacc tgttcctccc gccccagggt gggggcaagc 2161 cgcccagctc cgcacagacc gcagagatct tccaggcgct acagcaggag tgcatgaggc 2221 aactgcaggc tccggccggc tccccggccc cctctcccag cccggggggt gacgacaagc 2281 cccaggtgcc tcctcgggta cccatccccc ctcggcccac gcgcccacac gtccagctgt 2341 ctccagcccc cccgggcgag gaggagacca gccagtggcc tggacctgct tcccctcccc 2401 gggtgcctcc gcgggagccc ctgtcccctc aaggctcgag gacacccagc cccctggtac 2461 cacctggcag ctccccgctg ccaccccggc tctcaagctc acctgggaag accatgccca 2521 ccacccagag ctttgcctca gaccccaagt acgccacccc ccaggtgatc caggcccctg 2581 gcccgcgggc tggtccctgc atcctgccca tcgtccggga tggcaagaag gtcagcagca 2641 cccactatta cttgctgccc gagcgaccat cctacctgga gcgctaccag cgcttcctgc 2701 gtgaggccca gagccccgag gagcctaccc ccctgcctgt gcctctgctg ctgcccccac 2761 ccagcacccc agcccccgcc gcccccacgg ccaccgtgcg gccgatgccc caggctgcct 2821 tggaccccaa ggccaacttc TCCACCAACA ACAGCAACCC AGGGGCCCGG CCACCACCCC 2881 CGAGGGCCAC TGCTCGGCTG CCACAGAGGG GCTGCCCTGG CGATGGGCCA GAGGCGGGCC 2941 GGCCAGCAGA CAAGATCCAG ATGGCCATGG TGCATGGGGT GACCACAGAG GAGTGCCAGG 3001 CGGCCCTGCA GTGCCACGGC TGGAGCGTGC AGAGGGCTGC CCAGTATCTG AAGGTGGAGC 3061 AGCTCTTCGG GCTGGGTCTG CGGCCCAGAG GGGAGTGCCA CAAAGTGCTG GAGATGTTCG 3121 ACTGGAACCT GGAGCAGGCC GGCTGCCACC TTCTGGGCTC CTGGGGCCCT GCCCACCACA 3181 AGCGCTGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3241 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3301 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCGAA TTATCCTTTT GCTGGTCTAC 3361 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt