Construct: ORF TRCN0000488800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021066.1_s317c1
- DNA Barcode:
- CTAACTCGTCTTCGGTTCTGTGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRM1 (2911)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488800
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2911 | GRM1 | glutamate metabotropic rece... | NM_001278064.2 | 99.9% | 100% | 3168T>G |
| 2 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_011535782.1 | 99.9% | 100% | 3168T>G |
| 3 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010783.1 | 99.9% | 100% | 3168T>G |
| 4 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010784.1 | 99.9% | 100% | 3168T>G |
| 5 | human | 2911 | GRM1 | glutamate metabotropic rece... | NM_001278067.1 | 76% | 74.6% | 2660_2661ins35;2724_2725ins823 |
| 6 | human | 2911 | GRM1 | glutamate metabotropic rece... | NM_001278065.2 | 74.9% | 74.7% | (many diffs) |
| 7 | human | 2911 | GRM1 | glutamate metabotropic rece... | NM_001278066.1 | 74.9% | 74.7% | (many diffs) |
| 8 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010785.1 | 74.9% | 74.7% | (many diffs) |
| 9 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010786.1 | 74.9% | 74.7% | (many diffs) |
| 10 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010787.1 | 74.9% | 74.7% | (many diffs) |
| 11 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_024446412.1 | 74.9% | 74.7% | (many diffs) |
| 12 | human | 2911 | GRM1 | glutamate metabotropic rece... | XM_017010788.1 | 65.7% | 65.7% | 0_1ins1227;1941T>G |
| 13 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | NM_016976.3 | 87.4% | 94% | (many diffs) |
| 14 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | XM_006512549.3 | 87.4% | 94% | (many diffs) |
| 15 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | XM_006512550.3 | 69.7% | 74.7% | (many diffs) |
| 16 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | XM_006512551.3 | 67.3% | 71.7% | (many diffs) |
| 17 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | NM_001114333.2 | 66.9% | 72.8% | (many diffs) |
| 18 | mouse | 14816 | Grm1 | glutamate receptor, metabot... | XM_006512554.3 | 57.1% | 60.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3654
- ORF length:
- 3582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtcggg ctccttttgt tttttttccc agcgatcttt ttggaggtgt 121 cccttctccc cagaagcccc ggcaggaaag tgttgctggc aggagcgtcg tctcagcgct 181 cggtggccag aatggacgga gatgtcatca ttggagccct cttctcagtc catcaccagc 241 ctccggccga gaaagtgccc gagaggaagt gtggggagat cagggagcag tatggcatcc 301 agagggtgga ggccatgttc cacacgttgg ataagatcaa cgcggacccg gtcctcctgc 361 ccaacatcac cctgggcagt gagatccggg actcctgctg gcactcttcc gtggctctgg 421 aacagagcat tgagttcatt agggactctc tgatttccat tcgagatgag aaggatggga 481 tcaaccggtg tctgcctgac ggccagtccc tccccccagg caggactaag aagcccattg 541 cgggagtgat cggtcccggc tccagctctg tagccattca agtgcagaac ctgctccagc 601 tcttcgacat cccccagatc gcttattcag ccacaagcat cgacctgagt gacaaaactt 661 tgtacaaata cttcctgagg gttgtccctt ctgacacttt gcaggcaagg gccatgcttg 721 acatagtcaa acgttacaat tggacctatg tctctgcagt ccacacggaa gggaattatg 781 gggagagcgg aatggacgct ttcaaagagc tggctgccca ggaaggcctc tgtatcgccc 841 attctgacaa aatctacagc aacgctgggg agaagagctt tgaccgactc ttgcgcaaac 901 tccgagagag gcttcccaag gctagagtgg tggtctgctt ctgtgaaggc atgacagtgc 961 gaggactcct gagcgccatg cggcgccttg gcgtcgtggg cgagttctca ctcattggaa 1021 gtgatggatg ggcagacaga gatgaagtca ttgaaggtta tgaggtggaa gccaacgggg 1081 gaatcacgat aaagctgcag tctccagagg tcaggtcatt tgatgattat ttcctgaaac 1141 tgaggctgga cactaacacg aggaatccct ggttccctga gttctggcaa catcggttcc 1201 agtgccgcct tccaggacac cttctggaaa atcccaactt taaacgaatc tgcacaggca 1261 atgaaagctt agaagaaaac tatgtccagg acagtaagat ggggtttgtc atcaatgcca 1321 tctatgccat ggcacatggg ctgcagaaca tgcaccatgc cctctgccct ggccacgtgg 1381 gcctctgcga tgccatgaag cccatcgacg gcagcaagct gctggacttc ctcatcaagt 1441 cctcattcat tggagtatct ggagaggagg tgtggtttga tgagaaagga gacgctcctg 1501 gaaggtatga tatcatgaat ctgcagtaca ctgaagctaa tcgctatgac tatgtgcacg 1561 ttggaacctg gcatgaagga gtgctgaaca ttgatgatta caaaatccag atgaacaaga 1621 gtggagtggt gcggtctgtg tgcagtgagc cttgcttaaa gggccagatt aaggttatac 1681 ggaaaggaga agtgagctgc tgctggattt gcacggcctg caaagagaat gaatatgtgc 1741 aagatgagtt cacctgcaaa gcttgtgact tgggatggtg gcccaatgca gatctaacag 1801 gctgtgagcc cattcctgtg cgctatcttg agtggagcaa catcgaatcc attatagcca 1861 tcgccttttc atgcctggga atccttgtta ccttgtttgt caccctaatc tttgtactgt 1921 accgggacac accagtggtc aaatcctcca gtcgggagct ctgctacatc atcctagctg 1981 gcatcttcct tggttatgtg tgcccattca ctctcattgc caaacctact accacctcct 2041 gctacctcca gcgcctcttg gttggcctct cctctgcgat gtgctactct gctttagtga 2101 ctaaaaccaa tcgtattgca cgcatcctgg ctggcagcaa gaagaagatc tgcacccgga 2161 agcccaggtt catgagtgcc tgggctcagg tgatcattgc ctcaattctg attagtgtgc 2221 aactaaccct ggtggtaacc ctgatcatca tggaaccccc tatgcccatt ctgtcctacc 2281 caagtatcaa ggaagtctac cttatctgca ataccagcaa cctgggtgtg gtggcccctt 2341 tgggctacaa tggactcctc atcatgagct gtacctacta tgccttcaag acccgcaacg 2401 tgcccgccaa cttcaacgag gccaaatata tcgcgttcac catgtacacc acctgtatca 2461 tctggctagc ttttgtgccc atttactttg ggagcaacta caagatcatc acaacttgct 2521 ttgcagtgag tctcagtgta acagtggctc tggggtgcat gttcactccc aagatgtaca 2581 tcattattgc caagcctgag aggaatgtcc gcagtgcctt caccacctct gatgttgtcc 2641 gcatgcatgt tggcgatggc aagctgccct gccgctccaa cactttcctc aacatcttcc 2701 gaagaaagaa ggcaggggca gggaatgcca attctaatgg caagtctgtg tcatggtctg 2761 aaccaggtgg aggacaggtg cccaagggac agcatatgtg gcaccgcctc tctgtgcacg 2821 tgaagaccaa tgagacggcc tgcaaccaaa cagccgtcat caagcccctc actaaaagtt 2881 accaaggctc tggcaagagc ctgacctttt cagataccag caccaagacc ctttacaacg 2941 tagaggagga ggaggatgcc cagccgattc gctttagccc gcctggtagc ccttccatgg 3001 tggtgcacag gcgcgtgcca agcgcggcga ccactccgcc tctgccgtcc cacctgaccg 3061 cagaggagac ccccctcttc ctggccgaac cagccctccc caagggcttg ccccctcctc 3121 tccagcagca gcagcaaccc cctccacagc agaaatcgct gatggaccag ctccagggag 3181 tggtcagcaa cttcagtacc gcgatcccgg attttcacgc ggtgctggca ggccccgggg 3241 gtcccgggaa cgggctgcgg tccctgtacc cgcccccgcc acctccgcag cacctgcaga 3301 tgctgccgct gcagctgagc acctttgggg aggagctggt ctccccgccc gcggacgacg 3361 acgacgacag cgagaggttt aagctccTCC AGGAGTACGT GTATGAGCAC GAGCGGGAAG 3421 GGAACACGGA AGAAGACGAA CTGGAAGAGG AGGAGGAGGA CCTGCAGGCG GCCAGCAAAC 3481 TGACCCCGGA TGATTCGCCT GCGCTGACGC CTCCGTCGCC TTTCCGCGAC TCGGTGGCCT 3541 CGGGCAGCTC GGTGCCCAGC TCCCCCGTGT CCGAGTCGGT GCTCTGCACC CCTCCCAACG 3601 TATCCTACGC CTCTGTCATT CTGCGGGACT ACAAGCAAAG CTCTTCCACC CTGTAAGACC 3661 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3721 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3781 ATATATCTTG TGGAAAGGAC GACTAACTCG TCTTCGGTTC TGTGCCACGC GTTAAGTCga 3841 caatcaacct ctggattaca aaatttgtga aagatt