Transcript: Mouse XM_006512549.3

PREDICTED: Mus musculus glutamate receptor, metabotropic 1 (Grm1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Grm1 (14816)
Length:
6912
CDS:
409..4008

Additional Resources:

NCBI RefSeq record:
XM_006512549.3
NBCI Gene record:
Grm1 (14816)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006512549.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000240439 GGCGCCAGCTGAATCATAAAT pLKO_005 4491 3UTR 100% 15.000 21.000 N LOC100045018 n/a
2 TRCN0000220804 GCCTCCATTCTGATTAGTGTA pLKO.1 2536 CDS 100% 4.950 6.930 N Grm1 n/a
3 TRCN0000025746 CCTGTAACCAAACAGCCGTAA pLKO.1 3176 CDS 100% 4.050 5.670 N Grm1 n/a
4 TRCN0000220805 GCCATGTTCCACACATTAGAT pLKO.1 649 CDS 100% 5.625 4.500 N Grm1 n/a
5 TRCN0000220803 CCGAGCATCAAGGAAGTCTAT pLKO.1 2617 CDS 100% 4.950 3.465 N Grm1 n/a
6 TRCN0000220806 GCCTGCAAAGAGAATGAGTTT pLKO.1 2053 CDS 100% 4.950 3.465 N Grm1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006512549.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488800 CTAACTCGTCTTCGGTTCTGTGCC pLX_317 7.7% 87.4% 94% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000489197 GGTACACCCGTGTGGTACCCGATA pLX_317 9.3% 87.4% 93.9% V5 (many diffs) n/a
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