Construct: ORF TRCN0000488803
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020680.1_s317c1
- DNA Barcode:
- CAAGTTGGGTTCATTTCCAAGGGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LATS1 (9113)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488803
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_004690.4 | 99.9% | 100% | 1446T>C;3042C>T |
| 2 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011474.1 | 97.1% | 97.1% | 1446T>C;2884_2982del;3141C>T |
| 3 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_024446583.1 | 97.1% | 97.1% | 1446T>C;2884_2982del;3141C>T |
| 4 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_001350340.2 | 90.4% | 90% | (many diffs) |
| 5 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011477.1 | 87.9% | 85.6% | (many diffs) |
| 6 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_001350339.2 | 87.8% | 87.4% | (many diffs) |
| 7 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_011536252.2 | 87% | 84% | (many diffs) |
| 8 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011479.1 | 86.2% | 84.7% | (many diffs) |
| 9 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_006715603.3 | 76.9% | 76.1% | (many diffs) |
| 10 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_001350392.2 | 75.1% | 75.2% | 0_1ins840;606T>C;2202C>T |
| 11 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011480.1 | 73% | 73% | (many diffs) |
| 12 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_001270519.1 | 60.3% | 59.9% | (many diffs) |
| 13 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011482.1 | 59.4% | 59.2% | (many diffs) |
| 14 | human | 9113 | LATS1 | large tumor suppressor kina... | NR_073033.1 | 45.5% | (many diffs) | |
| 15 | mouse | 16798 | Lats1 | large tumor suppressor | NM_010690.1 | 88% | 93% | (many diffs) |
| 16 | mouse | 16798 | Lats1 | large tumor suppressor | XM_006512567.1 | 51.5% | 53.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3462
- ORF length:
- 3390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagagg agtgaaaagc cagaaggata tagacaaatg aggcctaaga 121 cctttcctgc cagtaactat actgtcagta gccggcaaat gttacaagaa attcgggaat 181 cccttaggaa tttatctaaa ccatctgatg ctgctaaggc tgagcataac atgagtaaaa 241 tgtcaaccga agatcctcga caagtcagaa atccacccaa atttgggacg catcataaag 301 ccttgcagga aattcgaaac tctctgcttc catttgcaaa tgaaacaaat tcttctcgga 361 gtacttcaga agttaatcca caaatgcttc aagacttgca agctgctgga tttgatgagg 421 atatggttat acaagctctt cagaaaacta acaacagaag tatagaagca gcaattgaat 481 tcattagtaa aatgagttac caagatcctc gacgagagca gatggctgca gcagctgcca 541 gacctattaa tgccagcatg aaaccaggga atgtgcagca atcagttaac cgcaaacaga 601 gctggaaagg ttctaaagaa tccttagttc ctcagaggca tggcccgcca ctaggagaaa 661 gtgtggccta tcattctgag agtcccaact cacagacaga tgtaggaaga cctttgtctg 721 gatctggtat atcagcattt gttcaagctc accctagcaa cggacagaga gtgaaccccc 781 caccaccacc tcaagtaagg agtgttactc ctccaccacc tccaagaggc cagactcccc 841 ctccaagagg tacaactcca cctccccctt catgggaacc aaactctcaa acaaagcgct 901 attctggaaa catggaatac gtaatctccc gaatctctcc tgtcccacct ggggcatggc 961 aagagggcta tcctccacca cctctcaaca cttcccccat gaatcctcct aatcaaggac 1021 agagaggcat tagttctgtt cctgttggca gacaaccaat catcatgcag agttctagca 1081 aatttaactt tccatcaggg agacctggaa tgcagaatgg tactggacaa actgatttca 1141 tgatacacca aaatgttgtc cctgctggca ctgtgaatcg gcagccacca cctccatatc 1201 ctctgacagc agctaatgga caaagccctt ctgctttaca aacaggggga tctgctgctc 1261 cttcgtcata tacaaatgga agtattcctc agtctatgat ggtgccaaac agaaatagtc 1321 ataacatgga actatataac attagtgtac ctggactgca aacaaattgg cctcagtcat 1381 cttctgctcc agcccagtca tccccgagca gtgggcatga aatccctaca tggcaaccta 1441 acataccagt gaggtcaaat tcttttaata acccattagg aaatagagca agtcactctg 1501 ctaattctca gccttccgct acaacagtca ctgcaattac accagctcct attcaacagc 1561 ctgtgaaaag tatgcgtgta ttaaaaccag agctacagac tgctttagca cctacacacc 1621 cttcttggat accacagcca attcaaactg ttcaacccag tccttttcct gagggaaccg 1681 cttcaaatgt gactgtgatg ccacctgttg ctgaagctcc aaactatcaa ggaccaccac 1741 caccctaccc aaaacatctg ctgcaccaaa acccatctgt tcctccatac gagtcaatca 1801 gtaagcctag caaagaggat cagccaagct tgcccaagga agatgagagt gaaaagagtt 1861 atgaaaatgt tgatagtggg gataaagaaa agaaacagat tacaacttca cctattactg 1921 ttaggaaaaa caagaaagat gaagagcgaa gggaatctcg tattcaaagt tattctcctc 1981 aagcatttaa attctttatg gagcaacatg tagaaaatgt actcaaatct catcagcagc 2041 gtctacatcg taaaaaacaa ttagagaatg aaatgatgcg ggttggatta tctcaagatg 2101 cccaggatca aatgagaaag atgctttgcc aaaaagaatc taattacatc cgtcttaaaa 2161 gggctaaaat ggacaagtct atgtttgtga agataaagac actaggaata ggagcatttg 2221 gtgaagtctg tctagcaaga aaagtagata ctaaggcttt gtatgcaaca aaaactcttc 2281 gaaagaaaga tgttcttctt cgaaatcaag tcgctcatgt taaggctgag agagatatcc 2341 tggctgaagc tgacaatgaa tgggtagttc gtctatatta ttcattccaa gataaggaca 2401 atttatactt tgtaatggac tacattcctg ggggtgatat gatgagccta ttaattagaa 2461 tgggcatctt tccagaaagt ctggcacgat tctacatagc agaacttacc tgtgcagttg 2521 aaagtgttca taaaatgggt tttattcata gagatattaa acctgataat attttgattg 2581 atcgtgatgg tcatattaaa ttgactgact ttggcctctg cactggcttc agatggacac 2641 acgattctaa gtactatcag agtggtgacc atccacggca agatagcatg gatttcagta 2701 atgaatgggg ggatccctca agctgtcgat gtggagacag actgaagcca ttagagcgga 2761 gagctgcacg ccagcaccag cgatgtctag cacattcttt ggttgggact cccaattata 2821 ttgcacctga agtgttgcta cgaacaggat acacacagtt gtgtgattgg tggagtgttg 2881 gtgttattct ttttgaaatg ttggtgggac aacctccttt cttggcacaa acaccattag 2941 aaacacaaat gaaggttatc aactggcaaa catctcttca cattccacca caagctaaac 3001 tcagtcctga agcttctgat cttattatta aactttgccg aggacccgaa gatcgcttag 3061 gcaagaatgg tgctgatgaa ataaaagctc atccattttt taaaacaatt gatttctcca 3121 gtgacctgag acagcagtct gcttcataca ttcctaaaat cacacaccca acagatacat 3181 caaattttga tcctgttgaT CCTGATAAAT TATGGAGTGA TGATAACGAG GAAGAAAATG 3241 TAAATGACAC TCTCAATGGA TGGTATAAAA ATGGAAAGCA TCCTGAACAT GCATTCTATG 3301 AATTTACCTT CCGAAGGTTT TTTGATGACA ATGGCTACCC ATATAATTAT CCGAAGCCTA 3361 TTGAATATGA ATACATTAAT TCACAAGGCT CAGAGCAGCA GTCGGATGAA GATGATCAAA 3421 ACACAGGCTC AGAGATTAAA AATCGCGATC TAGTATATGT TTAGAACCCA GCTTTCTTGT 3481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3601 GAAAGGACGA CAAGTTGGGT TCATTTCCAA GGGGACGCGT TAAGTCgaca atcaacctct 3661 ggattacaaa atttgtgaaa gatt