Construct: ORF TRCN0000488863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020574.1_s317c1
- DNA Barcode:
- GAAACGTACAGGTATTCTTTTTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SPEG (10290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003162.2 | 100% | 100% | |
| 2 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003161.2 | 94.2% | 93.8% | 507delA;510T>A;512_540del |
| 3 | human | 10290 | SPEG | striated muscle enriched pr... | NM_001173476.2 | 66.4% | 66.4% | 0_1ins171 |
| 4 | human | 10290 | SPEG | striated muscle enriched pr... | XM_024452526.1 | 66.4% | 66.4% | 0_1ins171 |
| 5 | human | 10290 | SPEG | striated muscle enriched pr... | XR_001738587.2 | 10.5% | (many diffs) | |
| 6 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003159.2 | 9.2% | 9.1% | 506_513delCACACCCT;515_516delAAinsCG;519_5490del |
| 7 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246239.2 | 6.8% | 6.7% | 506_513delCACACCCT;515_516delAAinsCG;519_7425del |
| 8 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246242.4 | 6.4% | 6% | (many diffs) |
| 9 | human | 10290 | SPEG | striated muscle enriched pr... | XM_006712189.3 | 5.3% | 5.3% | (many diffs) |
| 10 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246237.2 | 5.3% | 5.2% | (many diffs) |
| 11 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003157.1 | 5.3% | 5.2% | (many diffs) |
| 12 | human | 10290 | SPEG | striated muscle enriched pr... | XM_011510483.2 | 5.3% | 5.2% | (many diffs) |
| 13 | human | 10290 | SPEG | striated muscle enriched pr... | NM_005876.5 | 5.1% | 5.1% | (many diffs) |
| 14 | human | 10290 | SPEG | striated muscle enriched pr... | XM_011510479.2 | 5.1% | 5.1% | (many diffs) |
| 15 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246240.2 | 4.5% | 4.4% | (many diffs) |
| 16 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246241.1 | 4.5% | 4.4% | (many diffs) |
| 17 | human | 10290 | SPEG | striated muscle enriched pr... | XM_006712193.3 | 4.5% | 4.4% | (many diffs) |
| 18 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003158.2 | 4.5% | 4.4% | (many diffs) |
| 19 | mouse | 11790 | Speg | SPEG complex locus | NM_001173477.1 | 58.2% | 62.9% | (many diffs) |
| 20 | mouse | 11790 | Speg | SPEG complex locus | XM_017312276.1 | 17.6% | 18.8% | (many diffs) |
| 21 | mouse | 11790 | Speg | SPEG complex locus | NM_001085371.1 | 17.4% | 18.6% | (many diffs) |
| 22 | mouse | 11790 | Speg | SPEG complex locus | XM_011238651.2 | 15.3% | 16.4% | (many diffs) |
| 23 | mouse | 11790 | Speg | SPEG complex locus | XM_011238650.2 | 8.5% | 9.1% | (many diffs) |
| 24 | mouse | 11790 | Speg | SPEG complex locus | NM_001085370.1 | 5.9% | 6.2% | (many diffs) |
| 25 | mouse | 11790 | Speg | SPEG complex locus | XM_006496400.2 | 5.9% | 6.2% | (many diffs) |
| 26 | mouse | 11790 | Speg | SPEG complex locus | XM_006496401.2 | 5.9% | 6.2% | (many diffs) |
| 27 | mouse | 11790 | Speg | SPEG complex locus | XM_011238648.2 | 5.9% | 6.2% | (many diffs) |
| 28 | mouse | 11790 | Speg | SPEG complex locus | XM_011238649.2 | 5.9% | 6.2% | (many diffs) |
| 29 | mouse | 11790 | Speg | SPEG complex locus | XM_006496402.2 | 5.7% | 5.7% | (many diffs) |
| 30 | mouse | 11790 | Speg | SPEG complex locus | XM_011238647.1 | 4.8% | 5.1% | (many diffs) |
| 31 | mouse | 11790 | Speg | SPEG complex locus | XM_017322375.1 | 4.7% | 5% | (many diffs) |
| 32 | mouse | 11790 | Speg | SPEG complex locus | XM_011238646.1 | 4.7% | 5% | (many diffs) |
| 33 | mouse | 11790 | Speg | SPEG complex locus | XM_011238645.1 | 4.7% | 5% | (many diffs) |
| 34 | mouse | 11790 | Speg | SPEG complex locus | XM_017322354.1 | 4.6% | 4.9% | (many diffs) |
| 35 | mouse | 11790 | Speg | SPEG complex locus | XM_011238644.1 | 4.6% | 4.9% | (many diffs) |
| 36 | mouse | 11790 | Speg | SPEG complex locus | NM_007463.4 | 4.5% | 4.8% | (many diffs) |
| 37 | mouse | 11790 | Speg | SPEG complex locus | XM_011238643.1 | 4.5% | 4.8% | (many diffs) |
| 38 | mouse | 11790 | Speg | SPEG complex locus | XM_006496403.3 | 3.9% | 4.2% | (many diffs) |
| 39 | mouse | 11790 | Speg | SPEG complex locus | XM_006496404.3 | 3.9% | 4.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 582
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccacc gccaccaacg agctgggcca ggccacctgt gccgcctcac 121 tgaccgtgag acccggtggg tctacatccc ctttcagcag ccccatcacc tccgacgagg 181 aatacctgag ccccccagag gagttcccag agcctgggga gacctggccg cgaaccccca 241 ccatgaagcc cagtcccagc cagaaccgcc gttcTTCTGA CACTGGCTCC AAGGCACCCC 301 CCACCTTCAA GGTCTCACTT ATGGACCAGT CAGTAAGAGA AGGCCAAGAT GTCATCATGA 361 GCATCCGCGT GCAGGGGGAG CCCAAGCCTG TGGTCTCCTG GCTGAGAAAC CGCCAGCCCG 421 TGCGCCCAGA CCAGCGGCGC TTTGCGGAGG AGGCTGAGGG TGGGCTGTGC CGGCTGCGGA 481 TCCTGGCTGC AGAGCGTGGC GATGCTGGTT TCTACACTTG CAAAGCGGTC AATGAGTATG 541 GTGCTCGGCA GTGCGAGGCC CGCTTGGAGG TCCGAGGCGA GTGAGACCCA GCTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA GAAACGTACA GGTATTCTTT TTCGACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt