Construct: ORF TRCN0000488897
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020647.1_s317c1
- DNA Barcode:
- CTGCGATCTAATACCCTCCTTAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ERBB4 (2066)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488897
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | NM_005235.3 | 100% | 100% | |
| 2 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_006712364.3 | 98.8% | 98.8% | 1871_1915del |
| 3 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_005246376.3 | 98.8% | 98.5% | (many diffs) |
| 4 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | NM_001042599.1 | 98.7% | 98.7% | 3135_3136ins48 |
| 5 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003578.2 | 98% | 98% | 557_634del |
| 6 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_005246377.3 | 97.6% | 97.3% | (many diffs) |
| 7 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003577.2 | 96.9% | 96.9% | 557_634del;1949_1993del |
| 8 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003580.2 | 96.9% | 96.6% | (many diffs) |
| 9 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003581.2 | 96.8% | 96.8% | 557_634del;3213_3214ins48 |
| 10 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003579.2 | 95.7% | 95.7% | 557_634del;1949_1993del;3258_3259ins48 |
| 11 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003582.1 | 83.1% | 83.1% | 1_1delAins622;1250_1294del |
| 12 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495692.3 | 90% | 97.3% | (many diffs) |
| 13 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495693.3 | 88.9% | 95.9% | (many diffs) |
| 14 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | NM_010154.2 | 88.8% | 96.1% | (many diffs) |
| 15 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495694.3 | 87.7% | 94.7% | (many diffs) |
| 16 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495695.3 | 57.5% | 62.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 4023
- ORF length:
- 3924
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccgtcg accaaaaaat gaagccggcg acaggacttt 121 gggtctgggt gagccttctc gtggcggcgg ggaccgtcca gcccagcgat tctcagtcag 181 tgtgtgcagg aacggagaat aaactgagct ctctctctga cctggaacag cagtaccgag 241 ccttgcgcaa gtactatgaa aactgtgagg ttgtcatggg caacctggag ataaccagca 301 ttgagcacaa ccgggacctc tccttcctgc ggtctgttcg agaagtcaca ggctacgtgt 361 tagtggctct taatcagttt cgttacctgc ctctggagaa tttacgcatt attcgtggga 421 caaaacttta tgaggatcga tatgccttgg caatattttt aaactacaga aaagatggaa 481 actttggact tcaagaactt ggattaaaga acttgacaga aatcctaaat ggtggagtct 541 atgtagacca gaacaaattc ctttgttatg cagacaccat tcattggcaa gatattgttc 601 ggaacccatg gccttccaac ttgactcttg tgtcaacaaa tggtagttca ggatgtggac 661 gttgccataa gtcctgtact ggccgttgct ggggacccac agaaaatcat tgccagactt 721 tgacaaggac ggtgtgtgca gaacaatgtg acggcagatg ctacggacct tacgtcagtg 781 actgctgcca tcgagaatgt gctggaggct gctcaggacc taaggacaca gactgctttg 841 cctgcatgaa tttcaatgac agtggagcat gtgttactca gtgtccccaa acctttgtct 901 acaatccaac cacctttcaa ctggagcaca atttcaatgc aaagtacaca tatggagcat 961 tctgtgtcaa gaaatgtcca cataactttg tggtagattc cagttcttgt gtgcgtgcct 1021 gccctagttc caagatggaa gtagaagaaa atgggattaa aatgtgtaaa ccttgcactg 1081 acatttgccc aaaagcttgt gatggcattg gcacaggatc attgatgtca gctcagactg 1141 tggattccag taacattgac aaattcataa actgtaccaa gatcaatggg aatttgatct 1201 ttctagtcac tggtattcat ggggaccctt acaatgcaat tgaagccata gacccagaga 1261 aactgaacgt ctttcggaca gtcagagaga taacaggttt cctgaacata cagtcatggc 1321 caccaaacat gactgacttc agtgtttttt ctaacctggt gaccattggt ggaagagtac 1381 tctatagtgg cctgtccttg cttatcctca agcaacaggg catcacctct ctacagttcc 1441 agtccctgaa ggaaatcagc gcaggaaaca tctatattac tgacaacagc aacctgtgtt 1501 attatcatac cattaactgg acaacactct tcagcacaat caaccagaga atagtaatcc 1561 gggacaacag aaaagctgaa aattgtactg ctgaaggaat ggtgtgcaac catctgtgtt 1621 ccagtgatgg ctgttgggga cctgggccag accaatgtct gtcgtgtcgc cgcttcagta 1681 gaggaaggat ctgcatagag tcttgtaacc tctatgatgg tgaatttcgg gagtttgaga 1741 atggctccat ctgtgtggag tgtgaccccc agtgtgagaa gatggaagat ggcctcctca 1801 catgccatgg accgggtcct gacaactgta caaagtgctc tcattttaaa gatggcccaa 1861 actgtgtgga aaaatgtcca gatggcttac agggggcaaa cagtttcatt ttcaagtatg 1921 ctgatccaga tcgggagtgc cacccatgcc atccaaactg cacccaaggg tgtaacggtc 1981 ccactagtca tgactgcatt tactacccat ggacgggcca ttccacttta ccacaacatg 2041 ctagaactcc cctgattgca gctggagtaa ttggtgggct cttcattctg gtcattgtgg 2101 gtctgacatt tgctgtttat gttagaagga agagcatcaa aaagaaaaga gccttgagaa 2161 gattcttgga aacagagttg gtggaaccat taactcccag tggcacagca cccaatcaag 2221 ctcaacttcg tattttgaaa gaaactgagc tgaagagggt aaaagtcctt ggctcaggtg 2281 cttttggaac ggtttataaa ggtatttggg tacctgaagg agaaactgtg aagattcctg 2341 tggctattaa gattcttaat gagacaactg gtcccaaggc aaatgtggag ttcatggatg 2401 aagctctgat catggcaagt atggatcatc cacacctagt ccggttgctg ggtgtgtgtc 2461 tgagcccaac catccagctg gttactcaac ttatgcccca tggctgcctg ttggagtatg 2521 tccacgagca caaggataac attggatcac aactgctgct taactggtgt gtccagatag 2581 ctaagggaat gatgtacctg gaagaaagac gactcgttca tcgggatttg gcagcccgta 2641 atgtcttagt gaaatctcca aaccatgtga aaatcacaga ttttgggcta gccagactct 2701 tggaaggaga tgaaaaagag tacaatgctg atggaggaaa gatgccaatt aaatggatgg 2761 ctctggagtg tatacattac aggaaattca cccatcagag tgacgtttgg agctatggag 2821 ttactatatg ggaactgatg acctttggag gaaaacccta tgatggaatt ccaacgcgag 2881 aaatccctga tttattagag aaaggagaac gtttgcctca gcctcccatc tgcactattg 2941 acgtttacat ggtcatggtc aaatgttgga tgattgatgc tgacagtaga cctaaattta 3001 aggaactggc tgctgagttt tcaaggatgg ctcgagaccc tcaaagatac ctagttattc 3061 agggtgatga tcgtatgaag cttcccagtc caaatgacag caagttcttt cagaatctct 3121 tggatgaaga ggatttggaa gatatgatgg atgctgagga gtacttggtc cctcaggctt 3181 tcaacatccc acctcccatc tatacttcca gagcaagaat tgactcgaat aggagtgaaa 3241 ttggacacag ccctcctcct gcctacaccc ccatgtcagg aaaccagttt gtataccgag 3301 atggaggttt tgctgctgaa caaggagtgt ctgtgcccta cagagcccca actagcacaa 3361 ttccagaagc tcctgtggca cagggtgcta ctgctgagat ttttgatgac tcctgctgta 3421 atggcaccct acgcaagcca gtggcacccc atgtccaaga ggacagtagc acccagaggt 3481 acagtgctga ccccaccgtg tttgccccag aacggagccc acgaggagag ctggatgagg 3541 aaggttacat gactcctatg cgagacaaac ccaaacaaga atacctgaat ccagtggagg 3601 agaacccttt tgtttctcgg agaaaaaatg gagaccttca agcattggat aatcccgaat 3661 atcacaatgc atccaatggt ccacccaagg ccgaggatga gtatgtGAAT GAGCCACTGT 3721 ACCTCAACAC CTTTGCCAAC ACCTTGGGAA AAGCTGAGTA CCTGAAGAAC AACATACTGT 3781 CAATGCCAGA GAAGGCCAAG AAAGCGTTTG ACAACCCTGA CTACTGGAAC CACAGCCTGC 3841 CACCTCGGAG CACCCTTCAG CACCCAGACT ACCTGCAGGA GTACAGCACA AAATATTTTT 3901 ATAAACAGAA TGGGCGGATC CGGCCTATTG TGGCAGAGAA TCCTGAATAC CTCTCTGAGT 3961 TCTCCCTGAA GCCAGGCACT GTGCTGCCGC CTCCACCTTA CAGACACCGG AATACTGTGG 4021 TGTAAAGATC TAAGGGTGGG CGCGCCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 4081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 4141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTGCGATC 4201 TAATACCCTC CTTAAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4261 aagatt