Construct: ORF TRCN0000488917
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019528.1_s317c1
- DNA Barcode:
- TATCTACGAACTTGGAGCTCCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK14 (1432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488917
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139012.2 | 100% | 100% | |
| 2 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_001315.2 | 94.6% | 96.1% | (many diffs) |
| 3 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010299.2 | 85.5% | 74.6% | (many diffs) |
| 4 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139014.2 | 85.2% | 70.4% | 762_763ins79;921_922ins80 |
| 5 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_011514310.3 | 83.9% | 82.2% | (many diffs) |
| 6 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139013.3 | 81.3% | 77.5% | (many diffs) |
| 7 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010300.2 | 80.5% | 70.9% | (many diffs) |
| 8 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010301.2 | 79.8% | 77.3% | (many diffs) |
| 9 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_006714998.3 | 78.6% | 78.6% | 0_1ins231 |
| 10 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010304.2 | 70.8% | 70.6% | 764_767delATGC;772_773delCCinsAA;774_775ins310 |
| 11 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010302.2 | 68.3% | 61.7% | (many diffs) |
| 12 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010303.2 | 67.3% | 65.2% | (many diffs) |
| 13 | human | 1432 | MAPK14 | mitogen-activated protein k... | XR_926065.2 | 54.5% | (many diffs) | |
| 14 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_011951.3 | 90.2% | 99.4% | (many diffs) |
| 15 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168508.1 | 85.5% | 95.5% | (many diffs) |
| 16 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168513.1 | 71.5% | 78.3% | (many diffs) |
| 17 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168514.1 | 71.5% | 78.3% | (many diffs) |
| 18 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | XM_017317480.1 | 67.1% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 1173
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tcccgctgga aaatgtctca ggagaggccc acgttctacc 121 ggcaggagct gaacaagaca atctgggagg tgcccgagcg ttaccagaac ctgtctccag 181 tgggctctgg cgcctatggc tctgtgtgtg ctgcttttga cacaaaaacg gggttacgtg 241 tggcagtgaa gaagctctcc agaccatttc agtccatcat tcatgcgaaa agaacctaca 301 gagaactgcg gttacttaaa catatgaaac atgaaaatgt gattggtctg ttggacgttt 361 ttacacctgc aaggtctctg gaggaattca atgatgtgta tctggtgacc catctcatgg 421 gggcagatct gaacaacatt gtgaaatgtc agaagcttac agatgaccat gttcagttcc 481 ttatctacca aattctccga ggtctaaagt atatacattc agctgacata attcacaggg 541 acctaaaacc tagtaatcta gctgtgaatg aagactgtga gctgaagatt ctggattttg 601 gactggctcg gcacacagat gatgaaatga caggctacgt ggccactagg tggtacaggg 661 ctcctgagat catgctgaac tggatgcatt acaaccagac agttgatatt tggtcagtgg 721 gatgcataat ggccgagctg ttgactggaa gaacattgtt tcctggtaca gaccatattg 781 atcagttgaa gctcatttta agactcgttg gaaccccagg ggctgagctt ttgaagaaaa 841 TCTCCTCAGA GTCTGCAAGA AACTATATTC AGTCTTTGAC TCAGATGCCG AAGATGAACT 901 TTGCGAATGT ATTTATTGGT GCCAATCCCC TGGCTGTCGA CTTGCTGGAG AAGATGCTTG 961 TATTGGACTC AGATAAGAGA ATTACAGCGG CCCAAGCCCT TGCACATGCC TACTTTGCTC 1021 AGTACCACGA TCCTGATGAT GAACCAGTGG CCGATCCTTA TGATCAGTCC TTTGAAAGCA 1081 GGGACCTCCT TATAGATGAG TGGAAAAGCC TGACCTATGA TGAAGTCATC AGCTTTGTGC 1141 CACCACCCCT TGACCAAGAA GAGATGGAGT CCAAGGGTGG GCGCGCCGAC CCAGCTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGATATCTAC GAACTTGGAG CTCCGGGACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt