Construct: ORF TRCN0000488993
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021523.1_s317c1
- DNA Barcode:
- TTATAGCGGGGCCCCGTCGCTGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HM13 (81502)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488993
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81502 | HM13 | histocompatibility minor 13 | NM_030789.3 | 99.9% | 99.7% | 704C>T |
| 2 | human | 81502 | HM13 | histocompatibility minor 13 | NM_178580.2 | 91.1% | 89.5% | (many diffs) |
| 3 | human | 81502 | HM13 | histocompatibility minor 13 | NM_178581.2 | 88.4% | 88.2% | 704C>T;1035_1181del |
| 4 | human | 81502 | HM13 | histocompatibility minor 13 | NM_178582.3 | 35.3% | 33.3% | (many diffs) |
| 5 | mouse | 14950 | H13 | histocompatibility 13 | NM_010376.4 | 91.3% | 95.5% | (many diffs) |
| 6 | mouse | 14950 | H13 | histocompatibility 13 | NM_001159551.1 | 85.5% | 86.5% | (many diffs) |
| 7 | mouse | 14950 | H13 | histocompatibility 13 | XM_006498783.3 | 80.8% | 84.5% | (many diffs) |
| 8 | mouse | 14950 | H13 | histocompatibility 13 | XR_374416.1 | 61% | (many diffs) | |
| 9 | mouse | 14950 | H13 | histocompatibility 13 | XR_001780858.1 | 54.2% | (many diffs) | |
| 10 | mouse | 14950 | H13 | histocompatibility 13 | XM_017315666.1 | 41% | 25.1% | (many diffs) |
| 11 | mouse | 14950 | H13 | histocompatibility 13 | NM_001159553.1 | 38.8% | 29.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1200
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggactcggcc ctcagcgatc cgcataacgg cagtgccgag gcaggcggcc 121 ccaccaacag cactacgcgg ccgccttcca cgcccgaggg catcgcgctg gcctacggca 181 gcctcctgct catggcgctg ctgcccatct tcttcggcgc cctgcgctcc gtacgctgcg 241 cccgcggcaa gaatgcttca gacatgcctg aaacaatcac cagccgggat gccgcccgct 301 tccccatcat cgccagctgc acactcttgg ggctctacct ctttttcaaa atattctccc 361 aggagtacat caacctcctg ctgtccatgt atttcttcgt gctgggaatc ctggccctgt 421 cccacaccat cagccccttc atgaataagt tttttccagc cagctttcca aatcgacagt 481 accagctgct cttcacacag ggttctgggg aaaacaagga agagatcatc aattatgaat 541 ttgacaccaa ggacctggtg tgcctgggcc tgagcagcat cgttggcgtc tggtacctgc 601 tgaggaagca ctggattgcc aacaaccttt ttggcctggc cttctccctt aatggagtag 661 agctcctgca cctcaacaat gtcagcactg gctgcatcct gctgggcgga ctcttcatct 721 acgatgtctt ctgggtattt ggcaccaatg tgatggtgac agtggccaag ttcttcgagg 781 caccaataaa attggtgttt ccccaggatc tgctggagaa aggcctcgaa gcaaacaact 841 ttgccatgct gggacttgga gatGTCGTCA TTCCAGGGAT CTTCATTGCC TTGCTGCTGC 901 GCTTTGACAT CAGCTTGAAG AAGAATACCC ACACCTACTT CTACACCAGC TTTGCAGCCT 961 ACATCTTCGG CCTGGGCCTT ACCATCTTCA TCATGCACAT CTTCAAGCAT GCTCAGCCTG 1021 CCCTCCTATA CCTGGTCCCC GCCTGCATCG GTTTTCCTGT CCTGGTGGCG CTGGCCAAGG 1081 GAGAAGTGAC AGAGATGTTC AGTTATGAGG AGTCAAATCC TAAGGATCCA GCGGCAGTGA 1141 CAGAATCCAA AGAGGGAACA GAGGCATCAG CATCGAAGGG GCTGGAGAAG AAAGAGAAAT 1201 AGAACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGATT ATAGCGGGGC CCCGTCGCTG GCACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt