Construct: ORF TRCN0000489071

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020616.1_s317c1
DNA Barcode:
TCACACATTATGCGGGCCCCCCCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
IGF1R (3480)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489071
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3480 IGF1R insulin like growth factor ... NM_000875.5 100% 100%
2 human 3480 IGF1R insulin like growth factor ... NM_001291858.2 99.9% 99.8% 2782_2783insCAG
3 human 3480 IGF1R insulin like growth factor ... XM_017022136.1 97.2% 96.4% (many diffs)
4 human 3480 IGF1R insulin like growth factor ... XM_017022137.1 97.2% 96.4% (many diffs)
5 human 3480 IGF1R insulin like growth factor ... XM_017022138.1 97.1% 96.3% (many diffs)
6 human 3480 IGF1R insulin like growth factor ... XM_017022139.1 91.1% 91.1% 0_1ins363
7 human 3480 IGF1R insulin like growth factor ... XM_011521516.2 77.8% 77.8% 0_1ins909
8 human 3480 IGF1R insulin like growth factor ... XM_024449913.1 77.8% 77.8% 0_1ins909
9 human 3480 IGF1R insulin like growth factor ... XM_011521517.2 67.4% 67.4% 0_1ins1335
10 mouse 16001 Igf1r insulin-like growth factor ... NM_010513.2 89.4% 95.9% (many diffs)
11 mouse 16001 Igf1r insulin-like growth factor ... XM_006540643.3 89.3% 95.9% (many diffs)
12 mouse 16001 Igf1r insulin-like growth factor ... XM_006540642.3 87.4% 93.8% (many diffs)
13 mouse 16001 Igf1r insulin-like growth factor ... XM_006540641.3 87.4% 93.7% (many diffs)
14 mouse 16001 Igf1r insulin-like growth factor ... XM_006540645.3 75.2% 80% (many diffs)
15 mouse 16001 Igf1r insulin-like growth factor ... XM_006540644.3 73.5% 78.2% (many diffs)
16 mouse 16001 Igf1r insulin-like growth factor ... XM_017321986.1 67.6% 73.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
4173
ORF length:
4101
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaagtct ggctccggag gagggtcccc gacctcgctg tgggggctcc
121tgtttctctc cgccgcgctc tcgctctggc cgacgagtgg agaaatctgc gggccaggca
181tcgacatccg caacgactat cagcagctga agcgcctgga gaactgcacg gtgatcgagg
241gctacctcca catcctgctc atctccaagg ccgaggacta ccgcagctac cgcttcccca
301agctcacggt cattaccgag tacttgctgc tgttccgagt ggctggcctc gagagcctcg
361gagacctctt ccccaacctc acggtcatcc gcggctggaa actcttctac aactacgccc
421tggtcatctt cgagatgacc aatctcaagg atattgggct ttacaacctg aggaacatta
481ctcggggggc catcaggatt gagaaaaatg ctgacctctg ttacctctcc actgtggact
541ggtccctgat cctggatgcg gtgtccaata actacattgt ggggaataag cccccaaagg
601aatgtgggga cctgtgtcca gggaccatgg aggagaagcc gatgtgtgag aagaccacca
661tcaacaatga gtacaactac cgctgctgga ccacaaaccg ctgccagaaa atgtgcccaa
721gcacgtgtgg gaagcgggcg tgcaccgaga acaatgagtg ctgccacccc gagtgcctgg
781gcagctgcag cgcgcctgac aacgacacgg cctgtgtagc ttgccgccac tactactatg
841ccggtgtctg tgtgcctgcc tgcccgccca acacctacag gtttgagggc tggcgctgtg
901tggaccgtga cttctgcgcc aacatcctca gcgccgagag cagcgactcc gaggggtttg
961tgatccacga cggcgagtgc atgcaggagt gcccctcggg cttcatccgc aacggcagcc
1021agagcatgta ctgcatccct tgtgaaggtc cttgcccgaa ggtctgtgag gaagaaaaga
1081aaacaaagac cattgattct gttacttctg ctcagatgct ccaaggatgc accatcttca
1141agggcaattt gctcattaac atccgacggg ggaataacat tgcttcagag ctggagaact
1201tcatggggct catcgaggtg gtgacgggct acgtgaagat ccgccattct catgccttgg
1261tctccttgtc cttcctaaaa aaccttcgcc tcatcctagg agaggagcag ctagaaggga
1321attactcctt ctacgtcctc gacaaccaga acttgcagca actgtgggac tgggaccacc
1381gcaacctgac catcaaagca gggaaaatgt actttgcttt caatcccaaa ttatgtgttt
1441ccgaaattta ccgcatggag gaagtgacgg ggactaaagg gcgccaaagc aaaggggaca
1501taaacaccag gaacaacggg gagagagcct cctgtgaaag tgacgtcctg catttcacct
1561ccaccaccac gtcgaagaat cgcatcatca taacctggca ccggtaccgg ccccctgact
1621acagggatct catcagcttc accgtttact acaaggaagc accctttaag aatgtcacag
1681agtatgatgg gcaggatgcc tgcggctcca acagctggaa catggtggac gtggacctcc
1741cgcccaacaa ggacgtggag cccggcatct tactacatgg gctgaagccc tggactcagt
1801acgccgttta cgtcaaggct gtgaccctca ccatggtgga gaacgaccat atccgtgggg
1861ccaagagtga gatcttgtac attcgcacca atgcttcagt tccttccatt cccttggacg
1921ttctttcagc atcgaactcc tcttctcagt taatcgtgaa gtggaaccct ccctctctgc
1981ccaacggcaa cctgagttac tacattgtgc gctggcagcg gcagcctcag gacggctacc
2041tttaccggca caattactgc tccaaagaca aaatccccat caggaagtat gccgacggca
2101ccatcgacat tgaggaggtc acagagaacc ccaagactga ggtgtgtggt ggggagaaag
2161ggccttgctg cgcctgcccc aaaactgaag ccgagaagca ggccgagaag gaggaggctg
2221aataccgcaa agtctttgag aatttcctgc acaactccat cttcgtgccc agacctgaaa
2281ggaagcggag agatgtcatg caagtggcca acaccaccat gtccagccga agcaggaaca
2341ccacggccgc agacacctac aacatcaccg acccggaaga gctggagaca gagtaccctt
2401tctttgagag cagagtggat aacaaggaga gaactgtcat ttctaacctt cggcctttca
2461cattgtaccg catcgatatc cacagctgca accacgaggc tgagaagctg ggctgcagcg
2521cctccaactt cgtctttgca aggactatgc ccgcagaagg agcagatgac attcctgggc
2581cagtgacctg ggagccaagg cctgaaaact ccatcttttt aaagtggccg gaacctgaga
2641atcccaatgg attgattcta atgtatgaaa taaaatacgg atcacaagtt gaggatcagc
2701gagaatgtgt gtccagacag gaatacagga agtatggagg ggccaagcta aaccggctaa
2761acccggggaa ctacacagcc cggattcagg ccacatctct ctctgggaat gggtcgtgga
2821cagatcctgt gttcttctat gtccaggcca aaacaggata tgaaaacttc atccatctga
2881tcatcgctct gcccgtcgct gtcctgttga tcgtgggagg gttggtgatt atgctgtacg
2941tcttccatag aaagagaaat aacagcaggc tggggaatgg agtgctgtat gcctctgtga
3001acccggagta cttcagcgct gctgatgtgt acgttcctga tgagtgggag gtggctcggg
3061agaagatcac catgagccgg gaacttgggc aggggtcgtt tgggatggtc tatgaaggag
3121ttgccaaggg tgtggtgaaa gatgaacctg aaaccagagt ggccattaaa acagtgaacg
3181aggccgcaag catgcgtgag aggattgagt ttctcaacga agcttctgtg atgaaggagt
3241tcaattgtca ccatgtggtg cgattgctgg gtgtggtgtc ccaaggccag ccaacactgg
3301tcatcatgga actgatgaca cggggcgatc tcaaaagtta tctccggtct ctgaggccag
3361aaatggagaa taatccagtc ctagcacctc caagcctgag caagatgatt cagatggccg
3421gagagattgc agacggcatg gcatacctca acgccaataa gttcgtccac agagaccttg
3481ctgcccggaa ttgcatggta gccgaagatt tcacagtcaa aatcggagat tttggtatga
3541cgcgagatat ctatgagaca gactattacc ggaaaggagg gaaagggctg ctgcccgtgc
3601gctggatgtc tcctgagtcc ctcaaggatg gagtcttcac cacttactcg gacgtctggt
3661ccttcggggt cgtcctctgg gagatcgcca cactggccga gcagccctac cagggcttgt
3721ccaacgagca agtccttcgc ttcgtcatgg agggcggcct tctggacaag ccagacaact
3781gtcctgacat gctgtttgaa ctgatgcgca tgtgctggca gtataacccc aagatgaggc
3841cttccttcct ggagatcatc agcagcatca aagaggagat ggagcctggc ttccggGAGG
3901TCTCCTTCTA CTACAGCGAG GAGAACAAGC TGCCCGAGCC GGAGGAGCTG GACCTGGAGC
3961CAGAGAACAT GGAGAGCGTC CCCCTGGACC CCTCGGCCTC CTCGTCCTCC CTGCCACTGC
4021CCGACAGACA CTCAGGACAC AAGGCCGAGA ACGGCCCCGG CCCTGGGGTG CTGGTCCTCC
4081GCGCCAGCTT CGACGAGAGA CAGCCTTACG CCCACATGAA CGGGGGCCGC AAGAACGAGC
4141GGGCCTTGCC GCTGCCCCAG TCTTCGACCT GCTAGAACCC AGCTTTCTTG TACAAAGTGG
4201TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
4261CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
4321ATCACACATT ATGCGGGCCC CCCCCACGCG TTAAGTCgac aatcaacctc tggattacaa
4381aatttgtgaa agatt

Download FASTA (ORF) (Full)