Construct: ORF TRCN0000489071
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020616.1_s317c1
- DNA Barcode:
- TCACACATTATGCGGGCCCCCCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- IGF1R (3480)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489071
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3480 | IGF1R | insulin like growth factor ... | NM_000875.5 | 100% | 100% | |
| 2 | human | 3480 | IGF1R | insulin like growth factor ... | NM_001291858.2 | 99.9% | 99.8% | 2782_2783insCAG |
| 3 | human | 3480 | IGF1R | insulin like growth factor ... | XM_017022136.1 | 97.2% | 96.4% | (many diffs) |
| 4 | human | 3480 | IGF1R | insulin like growth factor ... | XM_017022137.1 | 97.2% | 96.4% | (many diffs) |
| 5 | human | 3480 | IGF1R | insulin like growth factor ... | XM_017022138.1 | 97.1% | 96.3% | (many diffs) |
| 6 | human | 3480 | IGF1R | insulin like growth factor ... | XM_017022139.1 | 91.1% | 91.1% | 0_1ins363 |
| 7 | human | 3480 | IGF1R | insulin like growth factor ... | XM_011521516.2 | 77.8% | 77.8% | 0_1ins909 |
| 8 | human | 3480 | IGF1R | insulin like growth factor ... | XM_024449913.1 | 77.8% | 77.8% | 0_1ins909 |
| 9 | human | 3480 | IGF1R | insulin like growth factor ... | XM_011521517.2 | 67.4% | 67.4% | 0_1ins1335 |
| 10 | mouse | 16001 | Igf1r | insulin-like growth factor ... | NM_010513.2 | 89.4% | 95.9% | (many diffs) |
| 11 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540643.3 | 89.3% | 95.9% | (many diffs) |
| 12 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540642.3 | 87.4% | 93.8% | (many diffs) |
| 13 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540641.3 | 87.4% | 93.7% | (many diffs) |
| 14 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540645.3 | 75.2% | 80% | (many diffs) |
| 15 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540644.3 | 73.5% | 78.2% | (many diffs) |
| 16 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_017321986.1 | 67.6% | 73.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4173
- ORF length:
- 4101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagtct ggctccggag gagggtcccc gacctcgctg tgggggctcc 121 tgtttctctc cgccgcgctc tcgctctggc cgacgagtgg agaaatctgc gggccaggca 181 tcgacatccg caacgactat cagcagctga agcgcctgga gaactgcacg gtgatcgagg 241 gctacctcca catcctgctc atctccaagg ccgaggacta ccgcagctac cgcttcccca 301 agctcacggt cattaccgag tacttgctgc tgttccgagt ggctggcctc gagagcctcg 361 gagacctctt ccccaacctc acggtcatcc gcggctggaa actcttctac aactacgccc 421 tggtcatctt cgagatgacc aatctcaagg atattgggct ttacaacctg aggaacatta 481 ctcggggggc catcaggatt gagaaaaatg ctgacctctg ttacctctcc actgtggact 541 ggtccctgat cctggatgcg gtgtccaata actacattgt ggggaataag cccccaaagg 601 aatgtgggga cctgtgtcca gggaccatgg aggagaagcc gatgtgtgag aagaccacca 661 tcaacaatga gtacaactac cgctgctgga ccacaaaccg ctgccagaaa atgtgcccaa 721 gcacgtgtgg gaagcgggcg tgcaccgaga acaatgagtg ctgccacccc gagtgcctgg 781 gcagctgcag cgcgcctgac aacgacacgg cctgtgtagc ttgccgccac tactactatg 841 ccggtgtctg tgtgcctgcc tgcccgccca acacctacag gtttgagggc tggcgctgtg 901 tggaccgtga cttctgcgcc aacatcctca gcgccgagag cagcgactcc gaggggtttg 961 tgatccacga cggcgagtgc atgcaggagt gcccctcggg cttcatccgc aacggcagcc 1021 agagcatgta ctgcatccct tgtgaaggtc cttgcccgaa ggtctgtgag gaagaaaaga 1081 aaacaaagac cattgattct gttacttctg ctcagatgct ccaaggatgc accatcttca 1141 agggcaattt gctcattaac atccgacggg ggaataacat tgcttcagag ctggagaact 1201 tcatggggct catcgaggtg gtgacgggct acgtgaagat ccgccattct catgccttgg 1261 tctccttgtc cttcctaaaa aaccttcgcc tcatcctagg agaggagcag ctagaaggga 1321 attactcctt ctacgtcctc gacaaccaga acttgcagca actgtgggac tgggaccacc 1381 gcaacctgac catcaaagca gggaaaatgt actttgcttt caatcccaaa ttatgtgttt 1441 ccgaaattta ccgcatggag gaagtgacgg ggactaaagg gcgccaaagc aaaggggaca 1501 taaacaccag gaacaacggg gagagagcct cctgtgaaag tgacgtcctg catttcacct 1561 ccaccaccac gtcgaagaat cgcatcatca taacctggca ccggtaccgg ccccctgact 1621 acagggatct catcagcttc accgtttact acaaggaagc accctttaag aatgtcacag 1681 agtatgatgg gcaggatgcc tgcggctcca acagctggaa catggtggac gtggacctcc 1741 cgcccaacaa ggacgtggag cccggcatct tactacatgg gctgaagccc tggactcagt 1801 acgccgttta cgtcaaggct gtgaccctca ccatggtgga gaacgaccat atccgtgggg 1861 ccaagagtga gatcttgtac attcgcacca atgcttcagt tccttccatt cccttggacg 1921 ttctttcagc atcgaactcc tcttctcagt taatcgtgaa gtggaaccct ccctctctgc 1981 ccaacggcaa cctgagttac tacattgtgc gctggcagcg gcagcctcag gacggctacc 2041 tttaccggca caattactgc tccaaagaca aaatccccat caggaagtat gccgacggca 2101 ccatcgacat tgaggaggtc acagagaacc ccaagactga ggtgtgtggt ggggagaaag 2161 ggccttgctg cgcctgcccc aaaactgaag ccgagaagca ggccgagaag gaggaggctg 2221 aataccgcaa agtctttgag aatttcctgc acaactccat cttcgtgccc agacctgaaa 2281 ggaagcggag agatgtcatg caagtggcca acaccaccat gtccagccga agcaggaaca 2341 ccacggccgc agacacctac aacatcaccg acccggaaga gctggagaca gagtaccctt 2401 tctttgagag cagagtggat aacaaggaga gaactgtcat ttctaacctt cggcctttca 2461 cattgtaccg catcgatatc cacagctgca accacgaggc tgagaagctg ggctgcagcg 2521 cctccaactt cgtctttgca aggactatgc ccgcagaagg agcagatgac attcctgggc 2581 cagtgacctg ggagccaagg cctgaaaact ccatcttttt aaagtggccg gaacctgaga 2641 atcccaatgg attgattcta atgtatgaaa taaaatacgg atcacaagtt gaggatcagc 2701 gagaatgtgt gtccagacag gaatacagga agtatggagg ggccaagcta aaccggctaa 2761 acccggggaa ctacacagcc cggattcagg ccacatctct ctctgggaat gggtcgtgga 2821 cagatcctgt gttcttctat gtccaggcca aaacaggata tgaaaacttc atccatctga 2881 tcatcgctct gcccgtcgct gtcctgttga tcgtgggagg gttggtgatt atgctgtacg 2941 tcttccatag aaagagaaat aacagcaggc tggggaatgg agtgctgtat gcctctgtga 3001 acccggagta cttcagcgct gctgatgtgt acgttcctga tgagtgggag gtggctcggg 3061 agaagatcac catgagccgg gaacttgggc aggggtcgtt tgggatggtc tatgaaggag 3121 ttgccaaggg tgtggtgaaa gatgaacctg aaaccagagt ggccattaaa acagtgaacg 3181 aggccgcaag catgcgtgag aggattgagt ttctcaacga agcttctgtg atgaaggagt 3241 tcaattgtca ccatgtggtg cgattgctgg gtgtggtgtc ccaaggccag ccaacactgg 3301 tcatcatgga actgatgaca cggggcgatc tcaaaagtta tctccggtct ctgaggccag 3361 aaatggagaa taatccagtc ctagcacctc caagcctgag caagatgatt cagatggccg 3421 gagagattgc agacggcatg gcatacctca acgccaataa gttcgtccac agagaccttg 3481 ctgcccggaa ttgcatggta gccgaagatt tcacagtcaa aatcggagat tttggtatga 3541 cgcgagatat ctatgagaca gactattacc ggaaaggagg gaaagggctg ctgcccgtgc 3601 gctggatgtc tcctgagtcc ctcaaggatg gagtcttcac cacttactcg gacgtctggt 3661 ccttcggggt cgtcctctgg gagatcgcca cactggccga gcagccctac cagggcttgt 3721 ccaacgagca agtccttcgc ttcgtcatgg agggcggcct tctggacaag ccagacaact 3781 gtcctgacat gctgtttgaa ctgatgcgca tgtgctggca gtataacccc aagatgaggc 3841 cttccttcct ggagatcatc agcagcatca aagaggagat ggagcctggc ttccggGAGG 3901 TCTCCTTCTA CTACAGCGAG GAGAACAAGC TGCCCGAGCC GGAGGAGCTG GACCTGGAGC 3961 CAGAGAACAT GGAGAGCGTC CCCCTGGACC CCTCGGCCTC CTCGTCCTCC CTGCCACTGC 4021 CCGACAGACA CTCAGGACAC AAGGCCGAGA ACGGCCCCGG CCCTGGGGTG CTGGTCCTCC 4081 GCGCCAGCTT CGACGAGAGA CAGCCTTACG CCCACATGAA CGGGGGCCGC AAGAACGAGC 4141 GGGCCTTGCC GCTGCCCCAG TCTTCGACCT GCTAGAACCC AGCTTTCTTG TACAAAGTGG 4201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 4261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 4321 ATCACACATT ATGCGGGCCC CCCCCACGCG TTAAGTCgac aatcaacctc tggattacaa 4381 aatttgtgaa agatt