Construct: ORF TRCN0000489078
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020307.1_s317c1
- DNA Barcode:
- GTGGTACCGTTCCCTGATGAGAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK11 (6794)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489078
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6794 | STK11 | serine/threonine kinase 11 | NM_000455.5 | 100% | 100% | |
| 2 | human | 6794 | STK11 | serine/threonine kinase 11 | NM_000455.4:c.790_793del | 99.6% | 60.6% | 786_787insTTGT |
| 3 | mouse | 20869 | Stk11 | serine/threonine kinase 11 | NM_011492.4 | 84.2% | 89.4% | (many diffs) |
| 4 | mouse | 20869 | Stk11 | serine/threonine kinase 11 | NM_001301853.1 | 79.1% | 79.7% | (many diffs) |
| 5 | mouse | 20869 | Stk11 | serine/threonine kinase 11 | NM_001301854.1 | 59.9% | 62.7% | (many diffs) |
| 6 | mouse | 20869 | Stk11 | serine/threonine kinase 11 | XM_006513441.3 | 50.9% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1371
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggtg gtggacccgc agcagctggg catgttcacg gagggcgagc 121 tgatgtcggt gggtatggac acgttcatcc accgcatcga ctccaccgag gtcatctacc 181 agccgcgccg caagcgggcc aagctcatcg gcaagtacct gatgggggac ctgctggggg 241 aaggctctta cggcaaggtg aaggaggtgc tggactcgga gacgctgtgc aggagggccg 301 tcaagatcct caagaagaag aagttgcgaa ggatccccaa cggggaggcc aacgtgaaga 361 aggaaattca actactgagg aggttacggc acaaaaatgt catccagctg gtggatgtgt 421 tatacaacga agagaagcag aaaatgtata tggtgatgga gtactgcgtg tgtggcatgc 481 aggaaatgct ggacagcgtg ccggagaagc gtttcccagt gtgccaggcc cacgggtact 541 tctgtcagct gattgacggc ctggagtacc tgcatagcca gggcattgtg cacaaggaca 601 tcaagccggg gaacctgctg ctcaccaccg gtggcaccct caaaatctcc gacctgggcg 661 tggccgaggc actgcacccg ttcgcggcgg acgacacctg ccggaccagc cagggctccc 721 cggctttcca gccgcccgag attgccaacg gcctggacac cttctccggc ttcaaggtgg 781 acatctggtc ggctggggtc accctctaca acatcaccac gggtctgtac cccttcgaag 841 gggacaacat ctacaagttg tttgagaaca tcgggaaggg gagctacgcc atcccgggcg 901 actgtggccc cccgctctct gacctgctga aagggatgct tgagtacgaa ccggccaaga 961 ggttctccat ccggcagatc cggcagcaca gctggttccg gaagaaacat ccTCCGGCTG 1021 AAGCACCAGT GCCCATCCCA CCGAGCCCAG ACACCAAGGA CCGGTGGCGC AGCATGACTG 1081 TGGTGCCGTA CTTGGAGGAC CTGCACGGCG CGGACGAGGA CGAGGACCTC TTCGACATCG 1141 AGGATGACAT CATCTACACT CAGGACTTCA CGGTGCCCGG ACAGGTCCCA GAAGAGGAGG 1201 CCAGTCACAA TGGACAGCGC CGGGGCCTCC CCAAGGCCGT GTGTATGAAC GGCACAGAGG 1261 CGGCGCAGCT GAGCACCAAA TCCAGGGCGG AGGGCCGGGC CCCCAACCCT GCCCGCAAGG 1321 CCTGCTCCGC CAGCAGCAAG ATCCGCCGGC TGTCGGCCTG CAAGCAGCAG TGAGACCCAG 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAG TGGTACCGTT CCCTGATGAG AGAACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att