Construct: ORF TRCN0000489095
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021949.1_s317c1
- DNA Barcode:
- ACACCCGTTACCGGACACTAAACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489095
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2240_2257del | 99.9% | 100% | 2343G>A;3291C>A |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 99.4% | 99.4% | (many diffs) |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 95.7% | 95.6% | (many diffs) |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 95% | 95% | (many diffs) |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 92.1% | 89.8% | (many diffs) |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 88.4% | 86% | (many diffs) |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 76.8% | 74.5% | (many diffs) |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201284.2 | 56.4% | 51% | (many diffs) |
| 9 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201282.2 | 52.1% | 52% | 1881G>A;1883C>G;1884_1885ins1728 |
| 10 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201283.1 | 33.5% | 33.4% | 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2396 |
| 11 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 84.2% | 89.7% | (many diffs) |
| 12 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_007912.4 | 45.1% | 46.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3684
- ORF length:
- 3612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgaccc tccgggacgg ccggggcagc gctcctggcg ctgctggctg 121 cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc acgagtaaca 181 agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg atgttcaata 241 actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat tatgatcttt 301 ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc aacacagtgg 361 agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac gaaaattcct 421 atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag gagctgccca 481 tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac cctgccctgt 541 gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc agcaacatgt 601 cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc tgtcccaatg 661 ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc atctgtgccc 721 agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac aaccagtgtg 781 ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa ttccgagacg 841 aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc acgtaccaga 901 tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag aagtgtcccc 961 gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc gacagctatg 1021 agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt 1081 gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct acgaatatta 1141 aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg gtggcattta 1201 ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat attctgaaaa 1261 ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac aggacggacc 1321 tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat ggtcagtttt 1381 ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc aaggagataa 1441 gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat acaataaact 1501 ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac agaggtgaaa 1561 acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag ggctgctggg 1621 gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg 1681 acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct gagtgcatac 1741 agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga cggggaccag 1801 acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag acctgcccgg 1861 caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc ggccatgtgt 1921 gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt gaaggctgtc 1981 caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc 2041 tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc gttcggaagc 2101 gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca cccagtggag 2161 aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag 2221 tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca gaaggtgaga 2281 aagttaaaat tcccgtcgct atcaaggaat cgaaagccaa caaggaaatc ctcgatgaag 2341 cctacgtgat ggccagcgtg gacaaccccc acgtgtgccg cctgctgggc atctgcctca 2401 cctccaccgt gcaactcatc acgcagctca tgcccttcgg ctgcctcctg gactatgtcc 2461 gggaacacaa agacaatatt ggctcccagt acctgctcaa ctggtgtgtg cagatcgcaa 2521 agggcatgaa ctacttggag gaccgtcgct tggtgcaccg cgacctggca gccaggaacg 2581 tactggtgaa aacaccgcag catgtcaaga tcacagattt tgggctggcc aaactgctgg 2641 gtgcggaaga gaaagaatac catgcagaag gaggcaaagt gcctatcaag tggatggcat 2701 tggaatcaat tttacacaga atctataccc accagagtga tgtctggagc tacggggtga 2761 ccgtttggga gttgatgacc tttggatcca agccatatga cggaatccct gccagcgaga 2821 tctcctccat cctggagaaa ggagaacgcc tccctcagcc acccatatgt accatcgatg 2881 tctacatgat catggtcaag tgctggatga tagacgcaga tagtcgccca aagttccgtg 2941 agttgatcat cgaattctcc aaaatggccc gagaccccca gcgctacctt gtcattcagg 3001 gggatgaaag aatgcatttg ccaagtccta cagactccaa cttctaccgt gccctgatgg 3061 atgaagaaga catggacgac gtggtggatg ccgacgagta cctcatccca cagcagggct 3121 tcttcagcag cccctccacg tcacggactc ccctcctgag ctctctgagt gcaaccagca 3181 acaattccac cgtggcttgc attgatagaa atgggctgca aagctgtccc atcaaggaag 3241 acagcttctt gcagcgatac agctcagacc ccacaggcgc cttgactgag gacagcatag 3301 acgacacctt cctcccagtg cctgaataca taaaccagtc cgttcccaaa aggcccgctG 3361 GATCTGTGCA GAATCCTGTC TATCACAATC AGCCTCTGAA CCCCGCGCCC AGCAGAGACC 3421 CACACTACCA GGACCCCCAC AGCACTGCAG TGGGCAACCC CGAGTATCTC AACACTGTCC 3481 AGCCCACCTG TGTCAACAGC ACATTCGACA GCCCTGCCCA CTGGGCCCAG AAAGGCAGCC 3541 ACCAAATTAG CCTGGACAAC CCTGACTACC AGCAGGACTT CTTTCCCAAG GAAGCCAAGC 3601 CAAATGGCAT CTTTAAGGGC TCCACAGCTG AAAATGCAGA ATACCTAAGG GTCGCGCCAC 3661 AAAGCAGTGA ATTTATTGGA GCATGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3721 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCTA 3781 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AACACCCGTT 3841 ACCGGACACT AAACAACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 3901 agatt