Construct: ORF TRCN0000489126
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020170.1_s317c1
- DNA Barcode:
- CACCAATATGTCTGCCCACCGTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAPK14 (1432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489126
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139012.2 | 100% | 100% | |
| 2 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_001315.2 | 94.6% | 96.1% | (many diffs) |
| 3 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010299.2 | 85.5% | 74.6% | (many diffs) |
| 4 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139014.2 | 85.2% | 70.4% | 762_763ins79;921_922ins80 |
| 5 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_011514310.3 | 83.9% | 82.2% | (many diffs) |
| 6 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139013.3 | 81.3% | 77.5% | (many diffs) |
| 7 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010300.2 | 80.5% | 70.9% | (many diffs) |
| 8 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010301.2 | 79.8% | 77.3% | (many diffs) |
| 9 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_006714998.3 | 78.6% | 78.6% | 0_1ins231 |
| 10 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010304.2 | 70.8% | 70.6% | 764_767delATGC;772_773delCCinsAA;774_775ins310 |
| 11 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010302.2 | 68.3% | 61.7% | (many diffs) |
| 12 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010303.2 | 67.3% | 65.2% | (many diffs) |
| 13 | human | 1432 | MAPK14 | mitogen-activated protein k... | XR_926065.2 | 54.5% | (many diffs) | |
| 14 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_011951.3 | 90.2% | 99.4% | (many diffs) |
| 15 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168508.1 | 85.5% | 95.5% | (many diffs) |
| 16 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168513.1 | 71.5% | 78.3% | (many diffs) |
| 17 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168514.1 | 71.5% | 78.3% | (many diffs) |
| 18 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | XM_017317480.1 | 67.1% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1152
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctcag gagaggccca cgttctaccg gcaggagctg aacaagacaa 121 tctgggaggt gcccgagcgt taccagaacc tgtctccagt gggctctggc gcctatggct 181 ctgtgtgtgc tgcttttgac acaaaaacgg ggttacgtgt ggcagtgaag aagctctcca 241 gaccatttca gtccatcatt catgcgaaaa gaacctacag agaactgcgg ttacttaaac 301 atatgaaaca tgaaaatgtg attggtctgt tggacgtttt tacacctgca aggtctctgg 361 aggaattcaa tgatgtgtat ctggtgaccc atctcatggg ggcagatctg aacaacattg 421 tgaaatgtca gaagcttaca gatgaccatg ttcagttcct tatctaccaa attctccgag 481 gtctaaagta tatacattca gctgacataa ttcacaggga cctaaaacct agtaatctag 541 ctgtgaatga agactgtgag ctgaagattc tggattttgg actggctcgg cacacagatg 601 atgaaatgac aggctacgtg gccactaggt ggtacagggc tcctgagatc atgctgaact 661 ggatgcatta caaccagaca gttgatattt ggtcagtggg atgcataatg gccgagctgt 721 tgactggaag aacattgttt cctggtacag accatattga tcagttgaag ctcattttaa 781 gactcgttgg aaccccaggg gctgagcttt tgaagaaaat ctccTCAGAG TCTGCAAGAA 841 ACTATATTCA GTCTTTGACT CAGATGCCGA AGATGAACTT TGCGAATGTA TTTATTGGTG 901 CCAATCCCCT GGCTGTCGAC TTGCTGGAGA AGATGCTTGT ATTGGACTCA GATAAGAGAA 961 TTACAGCGGC CCAAGCCCTT GCACATGCCT ACTTTGCTCA GTACCACGAT CCTGATGATG 1021 AACCAGTGGC CGATCCTTAT GATCAGTCCT TTGAAAGCAG GGACCTCCTT ATAGATGAGT 1081 GGAAAAGCCT GACCTATGAT GAAGTCATCA GCTTTGTGCC ACCACCCCTT GACCAAGAAG 1141 AGATGGAGTC CTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1201 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1261 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CACCAATATG TCTGCCCACC 1321 GTGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt