Construct: ORF TRCN0000489137
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021808.1_s317c1
- DNA Barcode:
- TTACTCAGCCTTAGGATTTATCGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BTN3A3 (10384)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489137
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10384 | BTN3A3 | butyrophilin subfamily 3 me... | NM_006994.5 | 100% | 100% | |
| 2 | human | 10384 | BTN3A3 | butyrophilin subfamily 3 me... | NM_197974.3 | 91.6% | 91.6% | 0_1ins126;790_791ins21 |
| 3 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | NM_007048.6 | 79.3% | 74.8% | (many diffs) |
| 4 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XM_005248833.2 | 78.5% | 73.9% | (many diffs) |
| 5 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | NM_001145008.2 | 70.9% | 67.1% | (many diffs) |
| 6 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XM_006714983.3 | 64.1% | 60.4% | (many diffs) |
| 7 | human | 10384 | BTN3A3 | butyrophilin subfamily 3 me... | NM_001242803.2 | 64% | 63.8% | 0_1ins126;307_308ins504 |
| 8 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | NM_001145009.2 | 57.7% | 49.8% | (many diffs) |
| 9 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_001197246.2 | 54.3% | 52% | (many diffs) |
| 10 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_001197247.3 | 54.3% | 52% | (many diffs) |
| 11 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_007047.5 | 54.3% | 52% | (many diffs) |
| 12 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_005248827.4 | 54.3% | 52% | (many diffs) |
| 13 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010211.1 | 54.3% | 52% | (many diffs) |
| 14 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010212.1 | 54.3% | 52% | (many diffs) |
| 15 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | NM_194441.3 | 54.2% | 49.1% | (many diffs) |
| 16 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XM_005248834.4 | 54.2% | 49.1% | (many diffs) |
| 17 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_006714979.4 | 53.9% | 51.6% | (many diffs) |
| 18 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_006714980.4 | 53.9% | 51.6% | (many diffs) |
| 19 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_006714981.4 | 53.9% | 51.6% | (many diffs) |
| 20 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_011514267.3 | 53.9% | 51.6% | (many diffs) |
| 21 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_001197248.2 | 49.2% | 47.4% | (many diffs) |
| 22 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_001197249.2 | 47.2% | 45% | (many diffs) |
| 23 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010214.2 | 47.2% | 45% | (many diffs) |
| 24 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_006714982.4 | 46.8% | 44.6% | (many diffs) |
| 25 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_011514269.3 | 46.8% | 44.6% | (many diffs) |
| 26 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010213.2 | 46.8% | 44.6% | (many diffs) |
| 27 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_005248831.4 | 43.7% | 41.9% | (many diffs) |
| 28 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_005248832.4 | 43.7% | 41.9% | (many diffs) |
| 29 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XR_926039.3 | 42.6% | (many diffs) | |
| 30 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_011514270.3 | 39.1% | 37.3% | (many diffs) |
| 31 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_011514271.2 | 39.1% | 37.3% | (many diffs) |
| 32 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010215.2 | 39.1% | 37.3% | (many diffs) |
| 33 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010216.2 | 36.6% | 34.9% | (many diffs) |
| 34 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010217.2 | 32% | 30.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1821
- ORF length:
- 1752
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaaaatggca agttccctgg ctttccttct gctcaacttt catgtctccc 121 tcttcttggt ccagctgctc actccttgct cagctcagtt ttctgtgctt ggaccctctg 181 ggcccatcct ggccatggtg ggtgaagacg ctgatctgcc ctgtcacctg ttcccgacca 241 tgagtgcaga gaccatggag ctgaggtggg tgagttccag cctaaggcag gtggtgaacg 301 tgtatgcaga tggaaaggaa gtggaagaca ggcagagtgc accgtatcga gggagaactt 361 cgattctgcg ggatggcatc actgcaggga aggctgctct ccgaatacac aacgtcacag 421 cctctgacag tggaaagtac ttgtgttatt tccaagatgg tgacttctac gaaaaagccc 481 tggtggagct gaaggttgca gcattgggtt ctgatcttca cattgaagtg aagggttatg 541 aggatggagg gatccatctg gagtgcaggt ccactggctg gtacccccaa ccccaaataa 601 agtggagcga caccaaggga gagaacatcc cggctgtgga agcacctgtg gttgcagatg 661 gagtgggcct gtatgcagta gcagcatctg tgatcatgag aggcagctct ggtgggggtg 721 tatcctgcat catcagaaat tccctcctcg gcctggaaaa gacagccagc atatccatcg 781 cagacccctt cttcaggagc gcccagccct ggatcgcggc cctggcaggg accctgccta 841 tctcgttgct gcttctcgca ggagccagtt acttcttgtg gagacaacag aaggaaaaaa 901 ttgctctgtc cagggagaca gaaagagagc gagagatgaa agaaatggga tacgctgcaa 961 cagagcaaga aataagccta agagagaagc tccaggagga actcaagtgg aggaaaatcc 1021 agtacatggc tcgtggagag aagtctttgg cctatcatga atggaaaatg gccctcttca 1081 aacctgcgga tgtgattctg gatccagaca cggcaaacgc catcctcctt gtttctgagg 1141 accagaggag tgtgcagcgt gctgaagagc cgcgggatct gccagacaac cctgagagat 1201 ttgaatggcg ttactgtgtc cttggctgtg aaaacttcac atcagggaga cattactggg 1261 aggtggaagt gggggacaga aaagagtggc atattggggt atgtagtaag aacgtggaga 1321 ggaaaaaagg ttgggtcaaa atgacaccgg agaacggata ctggactatg ggcctgactg 1381 atgggaataa gtatcgggct ctcactgagc ccagaaccaa cctgaaactt cctgagcctc 1441 ctaggaaagt ggggaTCTTC CTGGACTATG AGACTGGAGA GATCTCGTTC TATAATGCCA 1501 CAGATGGATC TCATATCTAC ACCTTTCCGC ACGCCTCTTT CTCTGAGCCT CTATATCCTG 1561 TTTTCAGAAT TTTGACCTTG GAGCCCACTG CCCTGACCAT TTGCCCAATA CCAAAAGAAG 1621 TAGAGAGTTC CCCCGATCCT GACCTAGTGC CTGATCATTC CCTGGAGACA CCACTGACCC 1681 CGGGCTTAGC TAATGAAAGT GGGGAGCCTC AGGCTGAAGT AACATCTCTG CTTCTCCCTG 1741 CCCACCCTGG AGCTGAGGTC TCCCCTTCTG CAACAACCAA TCAGAACCAT AAGCTACAGG 1801 CACGCACTGA AGCACTTTAC TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1861 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1921 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TACTCAGCCT 1981 TAGGATTTAT CGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2041 att