Transcript: Human NM_001145009.2

Homo sapiens butyrophilin subfamily 3 member A1 (BTN3A1), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
BTN3A1 (11119)
Length:
4145
CDS:
331..1467

Additional Resources:

NCBI RefSeq record:
NM_001145009.2
NBCI Gene record:
BTN3A1 (11119)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001145009.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000163145 GCTGTGGATGGATCGCATATT pLKO.1 2513 3UTR 100% 13.200 18.480 N BTN3A1 n/a
2 TRCN0000160935 GTGGATGGATCGCATATTCAT pLKO.1 2516 3UTR 100% 5.625 7.875 N BTN3A1 n/a
3 TRCN0000158875 GCAACCAATCACAACCATAAA pLKO.1 2791 3UTR 100% 13.200 9.240 N BTN3A1 n/a
4 TRCN0000158933 GCACAATGAAGCAAGAACAAA pLKO.1 1217 CDS 100% 5.625 3.938 N BTN3A1 n/a
5 TRCN0000160841 GAGAGAGACATTCAGCCTATA pLKO.1 1298 CDS 100% 10.800 6.480 N BTN3A1 n/a
6 TRCN0000160127 CAGTAAGAATGTGCAGAGAAA pLKO.1 2323 3UTR 100% 4.950 2.970 N BTN3A1 n/a
7 TRCN0000161633 GCAGTAAGAATGTGCAGAGAA pLKO.1 2322 3UTR 100% 4.950 2.970 N BTN3A1 n/a
8 TRCN0000164463 CCTTCTGCTCAACTTTCGTGT pLKO.1 357 CDS 100% 2.640 1.584 N BTN3A1 n/a
9 TRCN0000123204 CCCTAGAGATTCTCTGTGATA pLKO.1 3567 3UTR 100% 4.950 2.475 Y BTN3A3 n/a
10 TRCN0000162141 CCCTAGAGATTCTCTGTGATA pLKO.1 3567 3UTR 100% 4.950 2.475 Y BTN3A1 n/a
11 TRCN0000061426 GAACGTGTATGCAGATGGAAA pLKO.1 558 CDS 100% 4.950 2.475 Y BTN3A2 n/a
12 TRCN0000161171 GCCTTCCTTCAATCAAGGTTT pLKO.1 3530 3UTR 100% 4.950 2.475 Y BTN3A1 n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3878 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3878 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001145009.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10209 pDONR223 100% 78.7% 72.4% None (many diffs) n/a
2 ccsbBroad304_10209 pLX_304 0% 78.7% 72.4% V5 (many diffs) n/a
3 TRCN0000480174 CACTCTCACGGGGATCCTTCGGCT pLX_317 36.8% 78.7% 72.4% V5 (many diffs) n/a
4 ccsbBroadEn_07755 pDONR223 100% 71.9% 66.7% None (many diffs) n/a
5 ccsbBroad304_07755 pLX_304 0% 71.9% 66.7% V5 (many diffs) n/a
6 TRCN0000489565 GTACGATGCCCACACCGAACAAGC pLX_317 22.8% 71.9% 66.7% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000489719 CATACACTGCGTGGTTCGTCGGTA pLX_317 25.4% 71.9% 66.6% V5 (many diffs) n/a
8 ccsbBroadEn_02417 pDONR223 100% 57.7% 49.8% None (many diffs) n/a
9 ccsbBroad304_02417 pLX_304 0% 57.7% 49.8% V5 (many diffs) n/a
10 TRCN0000468589 ACTAGCCCAGTTCCAATTTATGAG pLX_317 22.1% 57.7% 49.8% V5 (many diffs) n/a
11 TRCN0000489137 TTACTCAGCCTTAGGATTTATCGA pLX_317 21.3% 57.7% 49.8% V5 (not translated due to prior stop codon) (many diffs) n/a
12 TRCN0000489559 ACATCAGTGTCGACACGCCAGATT pLX_317 21.9% 57.6% 49.7% V5 (many diffs) n/a
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