Construct: ORF TRCN0000489143
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021491.1_s317c1
- DNA Barcode:
- GCAGAAAACAATTTGCACCACATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKCE (5581)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489143
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5581 | PRKCE | protein kinase C epsilon | NM_005400.3 | 100% | 100% | |
| 2 | human | 5581 | PRKCE | protein kinase C epsilon | XM_005264428.1 | 100% | 100% | |
| 3 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532971.3 | 86% | 84.1% | (many diffs) |
| 4 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532975.3 | 86% | 84.1% | (many diffs) |
| 5 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004486.2 | 86% | 84.1% | (many diffs) |
| 6 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532978.2 | 84.3% | 84.2% | 1_1delAins345;4_5delCA |
| 7 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004487.2 | 84.3% | 84.2% | 1_1delAins345;4_5delCA |
| 8 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004488.2 | 84.3% | 84.2% | 1_1delAins345;4_5delCA |
| 9 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532980.3 | 79.6% | 79.6% | 0_1ins450 |
| 10 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532981.3 | 79.6% | 79.6% | 0_1ins450 |
| 11 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004489.2 | 79.6% | 79.6% | 0_1ins450 |
| 12 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004490.2 | 79.6% | 79.6% | 0_1ins450 |
| 13 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004491.2 | 79.6% | 79.6% | 0_1ins450 |
| 14 | human | 5581 | PRKCE | protein kinase C epsilon | XM_024452991.1 | 79.6% | 79.6% | 0_1ins450 |
| 15 | human | 5581 | PRKCE | protein kinase C epsilon | XM_024452992.1 | 79.6% | 79.6% | 0_1ins450 |
| 16 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532982.2 | 67.4% | 67.4% | 0_1ins720 |
| 17 | human | 5581 | PRKCE | protein kinase C epsilon | XM_005264431.4 | 65.9% | 65.2% | (many diffs) |
| 18 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004492.2 | 65.1% | 64.9% | (many diffs) |
| 19 | human | 5581 | PRKCE | protein kinase C epsilon | XM_006712050.3 | 62.4% | 62.4% | 0_1ins831 |
| 20 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532983.2 | 58.2% | 57.2% | (many diffs) |
| 21 | human | 5581 | PRKCE | protein kinase C epsilon | XR_939695.2 | 54.7% | (many diffs) | |
| 22 | human | 5581 | PRKCE | protein kinase C epsilon | XR_001738846.2 | 18% | (many diffs) | |
| 23 | mouse | 18754 | Prkce | protein kinase C, epsilon | NM_011104.3 | 91% | 97.9% | (many diffs) |
| 24 | mouse | 18754 | Prkce | protein kinase C, epsilon | XM_017317328.1 | 85.6% | 91.7% | (many diffs) |
| 25 | mouse | 18754 | Prkce | protein kinase C, epsilon | XM_006523833.2 | 74.3% | 79.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2283
- ORF length:
- 2211
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtagtg ttcaatggcc ttcttaagat caaaatctgc gaggccgtga 121 gcttgaagcc cacagcctgg tcgctgcgcc atgcggtggg accccggccg cagactttcc 181 ttctcgaccc ctacattgcc ctcaatgtgg acgactcgcg catcggccaa acggccacca 241 agcagaagac caacagcccg gcctggcacg acgagttcgt caccgatgtg tgcaacggac 301 gcaagatcga gctggctgtc tttcacgatg cccccatagg ctacgacgac ttcgtggcca 361 actgcaccat ccagtttgag gagctgctgc agaacgggag ccgccacttc gaggactgga 421 ttgatctgga gccagaagga agagtgtatg tgatcatcga tctctcaggg tcgtcgggtg 481 aagcccctaa agacaatgaa gagcgtgtgt tcagggaacg catgcggccg aggaagcggc 541 agggggccgt caggcgcagg gtccatcagg tcaacggcca caagttcatg gccacctatc 601 ttcggcagcc cacctactgc tcccattgca gagacttcat ctggggtgtc ataggaaagc 661 agggatacca gtgtcaagtc tgcacctgcg tggtccacaa gcggtgccac gagctcataa 721 tcacaaagtg tgctgggtta aagaagcagg agacccccga ccaggtgggc tcccagcggt 781 tcagcgtcaa catgccccac aagttcggta tccacaacta caaggtccct accttctgcg 841 atcactgtgg gtccctgctc tggggactct tgcggcaggg tttgcagtgt aaagtctgca 901 aaatgaatgt tcaccgtcga tgtgagacca acgtggctcc caactgtgga gtggatgcca 961 gaggaatcgc caaagtactg gccgacctgg gcgttacccc agacaaaatc accaacagcg 1021 gccagagaag gaaaaagctc attgctggtg ccgagtcccc gcagcctgct tctggaagct 1081 caccatctga ggaagatcga tccaagtcag cacccacctc cccttgtgac caggaaataa 1141 aagaacttga gaacaacatt cggaaagcct tgtcatttga caaccgagga gaggagcacc 1201 gggcagcatc gtctcctgat ggccagctga tgagccccgg tgagaatggc gaagtccggc 1261 aaggccaggc caagcgcctg ggcctggatg agttcaactt catcaaggtg ttgggcaaag 1321 gcagctttgg caaggtcatg ttggcagaac tcaagggcaa agatgaagta tatgctgtga 1381 aggtcttaaa gaaggacgtc atccttcagg atgatgacgt ggactgcaca atgacagaga 1441 agaggatttt ggctctggca cggaaacacc cgtaccttac ccaactctac tgctgcttcc 1501 agaccaagga ccgcctcttt ttcgtcatgg aatatgtaaa tggtggagac ctcatgtttc 1561 agattcagcg ctcccgaaaa ttcgacgagc ctcgttcacg gttctatgct gcagaggtca 1621 catcggccct catgttcctc caccagcatg gagtcatcta cagggatttg aaactggaca 1681 acatccttct ggatgcagaa ggtcactgca agctggctga cttcgggatg tgcaaggaag 1741 ggattctgaa tggtgtgacg accaccacgt tctgtgggac tcctgactac atagctcctg 1801 agatcctgca ggagttggag tatggcccct ccgtggactg gtgggccctg ggggtgctga 1861 tgtacgagat gatggctgga cagcctccct ttgaggccga caatgaggac gacctatttg 1921 agtccatccT CCATGACGAC GTGCTGTACC CAGTCTGGCT CAGCAAGGAG GCTGTCAGCA 1981 TCTTGAAAGC TTTCATGACG AAGAATCCCC ACAAGCGCCT GGGCTGTGTG GCATCGCAGA 2041 ATGGCGAGGA CGCCATCAAG CAGCACCCAT TCTTCAAAGA GATTGACTGG GTGCTCCTGG 2101 AGCAGAAGAA GATCAAGCCA CCCTTCAAAC CACGCATTAA AACCAAAAGA GACGTCAATA 2161 ATTTTGACCA AGACTTTACC CGGGAAGAGC CGGTACTCAC CCTTGTGGAC GAAGCAATTG 2221 TAAAGCAGAT CAACCAGGAG GAATTCAAAG GTTTCTCCTA CTTTGGTGAA GACCTGATGC 2281 CCTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2341 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2401 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCAGAAAAC AATTTGCACC ACATCACGCG 2461 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt