Construct: ORF TRCN0000489148

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021006.1_s317c1
DNA Barcode:
ACCAAGACACGGACTTCTGAAGGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GRM7 (2917)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489148
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2917 GRM7 glutamate metabotropic rece... NM_000844.4 100% 100%
2 human 2917 GRM7 glutamate metabotropic rece... NM_181874.2 98.4% 97.9% (many diffs)
3 human 2917 GRM7 glutamate metabotropic rece... XM_017006273.1 81.6% 81.2% (many diffs)
4 human 2917 GRM7 glutamate metabotropic rece... XM_017006272.1 80.2% 79.2% (many diffs)
5 human 2917 GRM7 glutamate metabotropic rece... XM_024453492.1 53.2% 52.7% (many diffs)
6 human 2917 GRM7 glutamate metabotropic rece... XM_024453493.1 53.2% 52.7% (many diffs)
7 human 2917 GRM7 glutamate metabotropic rece... XM_024453494.1 53.2% 52.7% (many diffs)
8 human 2917 GRM7 glutamate metabotropic rece... XM_024453495.1 53.2% 52.7% (many diffs)
9 human 2917 GRM7 glutamate metabotropic rece... XM_024453497.1 53.2% 52.7% (many diffs)
10 human 2917 GRM7 glutamate metabotropic rece... XM_024453498.1 53.2% 52.7% (many diffs)
11 human 2917 GRM7 glutamate metabotropic rece... XR_001740136.2 48.4% 1_274del;2724_2747del;3044_5671del
12 human 2917 GRM7 glutamate metabotropic rece... XR_001740135.2 25.3% (many diffs)
13 human 2917 GRM7 glutamate metabotropic rece... XR_001740134.2 24.9% (many diffs)
14 human 2917 GRM7 glutamate metabotropic rece... XR_001740137.2 24.9% (many diffs)
15 mouse 108073 Grm7 glutamate receptor, metabot... NM_177328.3 91.6% 99.4% (many diffs)
16 mouse 108073 Grm7 glutamate receptor, metabot... XM_006505301.1 90.4% 97% (many diffs)
17 mouse 108073 Grm7 glutamate receptor, metabot... NM_001346640.1 90.1% 97.3% (many diffs)
18 mouse 108073 Grm7 glutamate receptor, metabot... XM_006505298.2 90.1% 97.3% (many diffs)
19 mouse 108073 Grm7 glutamate receptor, metabot... XM_006505299.3 90.1% 97.3% (many diffs)
20 mouse 108073 Grm7 glutamate receptor, metabot... XM_006505300.2 90.1% 96.4% (many diffs)
21 mouse 108073 Grm7 glutamate receptor, metabot... XM_006505296.1 90% 96.4% (many diffs)
22 mouse 108073 Grm7 glutamate receptor, metabot... XM_017321298.1 90% 96.4% (many diffs)
23 mouse 108073 Grm7 glutamate receptor, metabot... XM_006505302.2 81.8% 88.7% (many diffs)
24 mouse 108073 Grm7 glutamate receptor, metabot... XR_868843.1 75.1% (many diffs)
25 mouse 108073 Grm7 glutamate receptor, metabot... XR_001785079.1 59.7% (many diffs)
26 mouse 108073 Grm7 glutamate receptor, metabot... XR_001785080.1 59.4% (many diffs)
27 mouse 108073 Grm7 glutamate receptor, metabot... XR_001785078.1 58.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2817
ORF length:
2745
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggtccag ctgaggaagc tgctccgcgt cctgactttg atgaagttcc
121cctgctgcgt gctggaggtg ctcctgtgcg cgctggcggc ggcggcgcgc ggccaggaga
181tgtacgcccc gcactcaatc cggatcgagg gggacgtcac cctcgggggg ctgttccccg
241tgcacgccaa gggtcccagc ggagtgccct gcggcgacat caagagggaa aacgggatcc
301acaggctgga agcgatgctc tacgccctgg accagatcaa cagtgatccc aacctactgc
361ccaacgtgac gctgggcgcg cggatcctgg acacttgttc cagggacact tacgcgctcg
421aacagtcgct tactttcgtc caggcgctca tccagaagga cacctccgac gtgcgctgca
481ccaacggcga accgccggtt ttcgtcaagc cggagaaagt agttggagtg attggggctt
541cggggagttc ggtctccatc atggtagcca acatcctgag gctcttccag atcccccaga
601ttagttatgc atcaacggca cccgagctaa gtgatgaccg gcgctatgac ttcttctctc
661gcgtggtgcc acccgattcc ttccaagccc aggccatggt agacattgta aaggccctag
721gctggaatta tgtgtctacc ctcgcatcgg aaggaagtta tggagagaaa ggtgtggagt
781ccttcacgca gatttccaaa gaggcaggtg gactctgcat tgcccagtcc gtgagaatcc
841cccaggaacg caaagacagg accattgact ttgatagaat tatcaaacag ctcctggaca
901cccccaactc cagggccgtc gtgatttttg ccaacgatga ggatataaag cagatccttg
961cagcagccaa aagagctgac caagttggcc attttctttg ggtgggatca gacagctggg
1021gatccaaaat aaacccactg caccagcatg aagatatcgc agaaggggcc atcaccattc
1081agcccaagcg agccacggtg gaagggtttg atgcctactt tacgtcccgt acacttgaaa
1141acaacagaag aaatgtatgg tttgccgaat actgggagga aaacttcaac tgcaagttga
1201cgattagtgg gtcaaaaaaa gaagacacag atcgcaaatg cacaggacag gagagaattg
1261gaaaagattc caactatgag caggagggta aagtccagtt cgtgattgac gcagtctatg
1321ctatggctca cgcccttcac cacatgaaca aggatctctg tgctgactac cggggtgtct
1381gcccagagat ggagcaagct ggaggcaaga agttgctgaa gtatatacgc aatgttaatt
1441tcaatggtag tgctggcact ccagtgatgt ttaacaagaa cggggatgca cctgggcgtt
1501atgacatctt tcagtaccag accacaaaca ccagcaaccc gggttaccgt ctgatcgggc
1561agtggacaga cgaacttcag ctcaatatag aagacatgca gtggggtaaa ggagtccgag
1621agatacccgc ctcagtgtgc acactaccat gtaagccagg acagagaaag aagacacaga
1681aaggaactcc ttgctgttgg acctgtgagc cttgcgatgg ttaccagtac cagtttgatg
1741agatgacatg ccagcattgc ccctatgacc agaggcccaa tgaaaatcga accggatgcc
1801aggatattcc catcatcaaa ctggagtggc actccccctg ggctgtgatt cctgtcttcc
1861tggcaatgtt ggggatcatt gccaccatct ttgtcatggc cactttcatc cgctacaatg
1921acacgcccat tgtccgggca tctgggcggg aactcagcta tgttcttttg acgggcatct
1981ttctttgcta catcatcact ttcctgatga ttgccaaacc agatgtggca gtgtgttctt
2041tccggcgagt tttcttgggc ttgggtatgt gcatcagtta tgcagccctc ttgacgaaaa
2101caaatcggat ttatcgcata tttgagcagg gcaagaaatc agtaacagct cccagactca
2161taagcccaac atcacaactg gcaatcactt ccagtttaat atcagttcag cttctagggg
2221tgttcatttg gtttggtgtt gatccaccca acatcatcat agactatgat gaacacaaga
2281caatgaaccc tgagcaagcc agaggggttc tcaagtgtga cattacagat ctccaaatca
2341tttgctcctt gggatatagc attcttctca tggtcacatg tactgtgtat GCCATCAAGA
2401CTCGGGGTGT ACCCGAGAAT TTTAACGAAG CCAAGCCCAT TGGATTCACT ATGTACACGA
2461CATGTATAGT ATGGCTTGCC TTCATTCCAA TTTTTTTTGG CACCGCTCAA TCAGCGGAAA
2521AGCTCTACAT ACAAACTACC ACGCTTACAA TCTCCATGAA CCTAAGTGCA TCAGTGGCGC
2581TGGGGATGCT ATACATGCCG AAAGTGTACA TCATCATTTT CCACCCTGAA CTCAATGTCC
2641AGAAACGGAA GCGAAGCTTC AAGGCGGTAG TCACAGCAGC CACCATGTCA TCGAGGCTGT
2701CACACAAACC CAGTGACAGA CCCAACGGTG AGGCAAAGAC CGAGCTCTGT GAAAACGTAG
2761ACCCAAACAG CCCTGCTGCA AAAAAGAAGT ATGTCAGTTA TAATAACCTG GTTATCTAGA
2821ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
2881GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2941TTTATATATC TTGTGGAAAG GACGAACCAA GACACGGACT TCTGAAGGAA CGCGTTAAGT
3001Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)

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