Construct: ORF TRCN0000489148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021006.1_s317c1
- DNA Barcode:
- ACCAAGACACGGACTTCTGAAGGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRM7 (2917)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489148
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2917 | GRM7 | glutamate metabotropic rece... | NM_000844.4 | 100% | 100% | |
| 2 | human | 2917 | GRM7 | glutamate metabotropic rece... | NM_181874.2 | 98.4% | 97.9% | (many diffs) |
| 3 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_017006273.1 | 81.6% | 81.2% | (many diffs) |
| 4 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_017006272.1 | 80.2% | 79.2% | (many diffs) |
| 5 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453492.1 | 53.2% | 52.7% | (many diffs) |
| 6 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453493.1 | 53.2% | 52.7% | (many diffs) |
| 7 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453494.1 | 53.2% | 52.7% | (many diffs) |
| 8 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453495.1 | 53.2% | 52.7% | (many diffs) |
| 9 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453497.1 | 53.2% | 52.7% | (many diffs) |
| 10 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453498.1 | 53.2% | 52.7% | (many diffs) |
| 11 | human | 2917 | GRM7 | glutamate metabotropic rece... | XR_001740136.2 | 48.4% | 1_274del;2724_2747del;3044_5671del | |
| 12 | human | 2917 | GRM7 | glutamate metabotropic rece... | XR_001740135.2 | 25.3% | (many diffs) | |
| 13 | human | 2917 | GRM7 | glutamate metabotropic rece... | XR_001740134.2 | 24.9% | (many diffs) | |
| 14 | human | 2917 | GRM7 | glutamate metabotropic rece... | XR_001740137.2 | 24.9% | (many diffs) | |
| 15 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | NM_177328.3 | 91.6% | 99.4% | (many diffs) |
| 16 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505301.1 | 90.4% | 97% | (many diffs) |
| 17 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | NM_001346640.1 | 90.1% | 97.3% | (many diffs) |
| 18 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505298.2 | 90.1% | 97.3% | (many diffs) |
| 19 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505299.3 | 90.1% | 97.3% | (many diffs) |
| 20 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505300.2 | 90.1% | 96.4% | (many diffs) |
| 21 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505296.1 | 90% | 96.4% | (many diffs) |
| 22 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_017321298.1 | 90% | 96.4% | (many diffs) |
| 23 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505302.2 | 81.8% | 88.7% | (many diffs) |
| 24 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XR_868843.1 | 75.1% | (many diffs) | |
| 25 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XR_001785079.1 | 59.7% | (many diffs) | |
| 26 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XR_001785080.1 | 59.4% | (many diffs) | |
| 27 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XR_001785078.1 | 58.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2817
- ORF length:
- 2745
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtccag ctgaggaagc tgctccgcgt cctgactttg atgaagttcc 121 cctgctgcgt gctggaggtg ctcctgtgcg cgctggcggc ggcggcgcgc ggccaggaga 181 tgtacgcccc gcactcaatc cggatcgagg gggacgtcac cctcgggggg ctgttccccg 241 tgcacgccaa gggtcccagc ggagtgccct gcggcgacat caagagggaa aacgggatcc 301 acaggctgga agcgatgctc tacgccctgg accagatcaa cagtgatccc aacctactgc 361 ccaacgtgac gctgggcgcg cggatcctgg acacttgttc cagggacact tacgcgctcg 421 aacagtcgct tactttcgtc caggcgctca tccagaagga cacctccgac gtgcgctgca 481 ccaacggcga accgccggtt ttcgtcaagc cggagaaagt agttggagtg attggggctt 541 cggggagttc ggtctccatc atggtagcca acatcctgag gctcttccag atcccccaga 601 ttagttatgc atcaacggca cccgagctaa gtgatgaccg gcgctatgac ttcttctctc 661 gcgtggtgcc acccgattcc ttccaagccc aggccatggt agacattgta aaggccctag 721 gctggaatta tgtgtctacc ctcgcatcgg aaggaagtta tggagagaaa ggtgtggagt 781 ccttcacgca gatttccaaa gaggcaggtg gactctgcat tgcccagtcc gtgagaatcc 841 cccaggaacg caaagacagg accattgact ttgatagaat tatcaaacag ctcctggaca 901 cccccaactc cagggccgtc gtgatttttg ccaacgatga ggatataaag cagatccttg 961 cagcagccaa aagagctgac caagttggcc attttctttg ggtgggatca gacagctggg 1021 gatccaaaat aaacccactg caccagcatg aagatatcgc agaaggggcc atcaccattc 1081 agcccaagcg agccacggtg gaagggtttg atgcctactt tacgtcccgt acacttgaaa 1141 acaacagaag aaatgtatgg tttgccgaat actgggagga aaacttcaac tgcaagttga 1201 cgattagtgg gtcaaaaaaa gaagacacag atcgcaaatg cacaggacag gagagaattg 1261 gaaaagattc caactatgag caggagggta aagtccagtt cgtgattgac gcagtctatg 1321 ctatggctca cgcccttcac cacatgaaca aggatctctg tgctgactac cggggtgtct 1381 gcccagagat ggagcaagct ggaggcaaga agttgctgaa gtatatacgc aatgttaatt 1441 tcaatggtag tgctggcact ccagtgatgt ttaacaagaa cggggatgca cctgggcgtt 1501 atgacatctt tcagtaccag accacaaaca ccagcaaccc gggttaccgt ctgatcgggc 1561 agtggacaga cgaacttcag ctcaatatag aagacatgca gtggggtaaa ggagtccgag 1621 agatacccgc ctcagtgtgc acactaccat gtaagccagg acagagaaag aagacacaga 1681 aaggaactcc ttgctgttgg acctgtgagc cttgcgatgg ttaccagtac cagtttgatg 1741 agatgacatg ccagcattgc ccctatgacc agaggcccaa tgaaaatcga accggatgcc 1801 aggatattcc catcatcaaa ctggagtggc actccccctg ggctgtgatt cctgtcttcc 1861 tggcaatgtt ggggatcatt gccaccatct ttgtcatggc cactttcatc cgctacaatg 1921 acacgcccat tgtccgggca tctgggcggg aactcagcta tgttcttttg acgggcatct 1981 ttctttgcta catcatcact ttcctgatga ttgccaaacc agatgtggca gtgtgttctt 2041 tccggcgagt tttcttgggc ttgggtatgt gcatcagtta tgcagccctc ttgacgaaaa 2101 caaatcggat ttatcgcata tttgagcagg gcaagaaatc agtaacagct cccagactca 2161 taagcccaac atcacaactg gcaatcactt ccagtttaat atcagttcag cttctagggg 2221 tgttcatttg gtttggtgtt gatccaccca acatcatcat agactatgat gaacacaaga 2281 caatgaaccc tgagcaagcc agaggggttc tcaagtgtga cattacagat ctccaaatca 2341 tttgctcctt gggatatagc attcttctca tggtcacatg tactgtgtat GCCATCAAGA 2401 CTCGGGGTGT ACCCGAGAAT TTTAACGAAG CCAAGCCCAT TGGATTCACT ATGTACACGA 2461 CATGTATAGT ATGGCTTGCC TTCATTCCAA TTTTTTTTGG CACCGCTCAA TCAGCGGAAA 2521 AGCTCTACAT ACAAACTACC ACGCTTACAA TCTCCATGAA CCTAAGTGCA TCAGTGGCGC 2581 TGGGGATGCT ATACATGCCG AAAGTGTACA TCATCATTTT CCACCCTGAA CTCAATGTCC 2641 AGAAACGGAA GCGAAGCTTC AAGGCGGTAG TCACAGCAGC CACCATGTCA TCGAGGCTGT 2701 CACACAAACC CAGTGACAGA CCCAACGGTG AGGCAAAGAC CGAGCTCTGT GAAAACGTAG 2761 ACCCAAACAG CCCTGCTGCA AAAAAGAAGT ATGTCAGTTA TAATAACCTG GTTATCTAGA 2821 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2881 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2941 TTTATATATC TTGTGGAAAG GACGAACCAA GACACGGACT TCTGAAGGAA CGCGTTAAGT 3001 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt