Construct: ORF TRCN0000489182

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021715.1_s317c1
DNA Barcode:
GGGCACACGGTCTCTGTGCCATAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
IGF1R (3480)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489182
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3480 IGF1R insulin like growth factor ... XM_011521517.2 41.5% .5% 1_1618del
2 human 3480 IGF1R insulin like growth factor ... XM_011521516.2 35.9% .4% 1_2044del
3 human 3480 IGF1R insulin like growth factor ... XM_024449913.1 35.9% .4% 1_2044del
4 human 3480 IGF1R insulin like growth factor ... XM_017022139.1 30.7% .4% 1_2590del
5 human 3480 IGF1R insulin like growth factor ... NM_001291858.2 28% .3% 1_2950del
6 human 3480 IGF1R insulin like growth factor ... NM_000875.5 27.9% .3% 1_2953del
7 human 3480 IGF1R insulin like growth factor ... XM_017022138.1 27.5% .3% 1_3025del
8 human 3480 IGF1R insulin like growth factor ... XM_017022136.1 27.4% .3% 1_3028del
9 human 3480 IGF1R insulin like growth factor ... XM_017022137.1 27.4% .3% 1_3028del
10 mouse 16001 Igf1r insulin-like growth factor ... XM_017321986.1 31.3% .5% (many diffs)
11 mouse 16001 Igf1r insulin-like growth factor ... XM_006540645.3 29.3% .4% (many diffs)
12 mouse 16001 Igf1r insulin-like growth factor ... XM_006540644.3 28.5% .4% (many diffs)
13 mouse 16001 Igf1r insulin-like growth factor ... NM_010513.2 25.1% .3% (many diffs)
14 mouse 16001 Igf1r insulin-like growth factor ... XM_006540643.3 25.1% .3% (many diffs)
15 mouse 16001 Igf1r insulin-like growth factor ... XM_006540642.3 24.5% .4% (many diffs)
16 mouse 16001 Igf1r insulin-like growth factor ... XM_006540641.3 24.5% .4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
85
ORF end:
133
ORF length:
48
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaattga
61acccggagta cttcagcgct gctgatgtgt acgttcctga tgagtgggag gtggctcggg
121agaagatcac catgagccgg gaacttgggc aggggtcgtt tgggatggtc tatgaaggag
181ttgccaaggg tgtggtgaaa gatgaacctg aaaccagagt ggccattaaa acagtgaacg
241aggccgcaag catgcgtgag aggattgagt ttctcaacga agcttctgtg atgaaggagt
301tcaattgtca ccatgtggtg cgattgctgg gtgtggtgtc ccaaggccag ccaacactgg
361tcatcatgga actgatgaca cggggcgatc tcaaaagtta tctccggtct ctgaggccag
421aaatggagaa taatccagtc ctagcacctc caagcctgag caagatgatt cagatggccg
481gagagattgc agacggcatg gcatacctca acgccaataa gttcgtccac agagaccttg
541ctgcccggaa ttgcatggta gccgaagatt tcacagtcaa aatcggagat tttggtatga
601cgcgagatat ctatgagaca gactattacc ggaaaggagg gaaagggctg ctgcccgtgc
661gctggatgtc tcctgagtcc ctcaaggatg gagtcttcac cacttactcg gacgtctggt
721ccttcggggt cgtcctctgg gagatcgcca cactggccga gcagccctac cagggcttgt
781ccaacgagca agtccttcgc ttcgtcatgg agggcggcct tctggacaag ccagacaact
841gtcctGACAT GCTGTTTGAA CTGATGCGCA TGTGCTGGCA GTATAACCCC AAGATGAGGC
901CTTCCTTCCT GGAGATCATC AGCAGCATCA AAGAGGAGAT GGAGCCTGGC TTCCGGGAGG
961TCTCCTTCTA CTACAGCGAG GAGAACAAGC TGCCCGAGCC GGAGGAGCTG GACCTGGAGC
1021CAGAGAACAT GGAGAGCGTC CCCCTGGACC CCTCGGCCTC CTCGTCCTCC CTGCCACTGC
1081CCGACAGACA CTCAGGACAC AAGGCCGAGA ACGGCCCCGG CCCTGGGGTG CTGGTCCTCC
1141GCGCCAGCTT CGACGAGAGA CAGCCTTACG CCCACATGAA CGGGGGCCGC AAGAACGAGC
1201GGGCCTTGCC GCTGCCCCAG TCTTCGACCT GCTAGGACCC AGCTTTCTTG TACAAAGTGG
1261TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1321CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1381AGGGCACACG GTCTCTGTGC CATACACGCG TTAAGTCgac aatcaacctc tggattacaa
1441aatttgtgaa agatt

Download FASTA (ORF) (Full)