Construct: ORF TRCN0000489189
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020697.1_s317c1
- DNA Barcode:
- GAAACGACAAGCGCAACCCGACTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NEK4 (6787)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489189
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_003157.6 | 99.8% | 99.8% | 312T>G;468A>G;673C>G |
| 2 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_011534039.3 | 96.5% | 94.3% | (many diffs) |
| 3 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001348412.2 | 94.4% | 94.4% | (many diffs) |
| 4 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_017007085.1 | 94.2% | 91.4% | (many diffs) |
| 5 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001348413.2 | 92% | 90.8% | (many diffs) |
| 6 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001348414.2 | 90.1% | 90.1% | (many diffs) |
| 7 | human | 6787 | NEK4 | NIMA related kinase 4 | NM_001193533.2 | 89.3% | 89.2% | 89_90ins267;201A>G;406C>G |
| 8 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_017007086.1 | 89% | 86.2% | (many diffs) |
| 9 | human | 6787 | NEK4 | NIMA related kinase 4 | XM_011534040.3 | 79.5% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2595
- ORF length:
- 2523
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcccctg gccgcctact gctacctgcg ggtcgtgggc aaggggagct 121 atggagaggt gacgcttgtg aagcaccggc gggacggcaa gcagtatgtc atcaaaaaac 181 tgaacctccg aaatgcctct agccgagagc ggcgagctgc tgaacaggaa gcccagctct 241 tgtctcagtt gaagcatccc aacattgtca cctacaagga gtcatgggaa ggaggagatg 301 gtctgctcta cattgtcatg ggcttctgtg aaggaggtga tttgtaccga aagctcaagg 361 agcagaaagg gcagcttctg ccggagaatc aggtggtaga gtggtttgta cagatcgcca 421 tggctttgca gtatttacat gaaaaacaca tccttcatcg agatctgaaa actcaaaatg 481 tcttcctaac aagaacaaac atcatcaaag taggggacct aggaattgcc cgagtgttgg 541 agaaccactg tgacatggct agcaccctca ttggcacacc ctactacatg agccctgaat 601 tgttctcaaa caaaccctac aactataagt ctgatgtttg ggctctagga tgctgtgtct 661 atgaaatggc caccttgaag catgctttca atgcaaaaga tatgaattct ttagtttatc 721 ggattattga aggaaagctg ccagcaatgc caagagatta cagcccagag ctggcagaac 781 tgataagaac aatgctgagc aaaaggcctg aagaaaggcc gtctgtgagg agcatcctga 841 ggcagcctta tataaagcgg caaatctcct tctttttgga ggccacaaag ataaaaacct 901 ccaaaaataa cattaaaaat ggtgactctc aatccaagcc ttttgctaca gtggtttctg 961 gagaggcaga atcaaatcat gaagtaatcc acccccaacc actctcttct gagggctccc 1021 agacatatat aatgggtgaa ggcaaatgtt tgtcccagga gaaacccagg gcctctggtc 1081 tcttgaagtc acctgccagt ctgaaagccc atacctgcaa acaggacttg agcaatacca 1141 cagaactagc cacaatcagt agcgtaaata ttgacatctt acctgcaaaa gggagggatt 1201 cagtgagtga tggctttgtt caggagaatc agccaagata tttggatgcc tctaatgagt 1261 taggaggtat atgcagtatt tctcaagtgg aagaggagat gctgcaggac aacactaaat 1321 ccagtgccca gcctgaaaac ctgattccca tgtggtcctc tgacattgtc actggggaaa 1381 agaatgaacc agtgaagcct ctgcagcccc taatcaaaga acaaaagcca aaggaccaga 1441 gtcttgccct gtcgcccaag ctggagtgca gtggcacaat cttggctcac agcaacctcc 1501 gcctcctggg ttcaagtgat tctccagcct cagcctcccg agtagctggg attacaggcg 1561 tgtgccacca cgcccaggat caagttgctg gtgaatgtat tatagaaaaa cagggcagaa 1621 tccacccaga tttacagcca cacaactctg ggtctgaacc ttccctgtct cgacagcgac 1681 ggcaaaagag gagagaacag actgagcaca gaggggaaaa gagacaggtc cgcagagatc 1741 tctttgcttt ccaagagtcg cctcctcgat ttttgccttc tcatcccatt gttgggaaag 1801 tggatgtcac atcaacacaa aaagaggctg aaaaccaacg tagagtggtc actgggtctg 1861 tgagcagttc aaggagcagt gagatgtcat catcaaagga tcgaccatta tcagccagag 1921 agaggaggcg actaaagcag tcacaggaag aaatgtcctc ttcaggccct tcagtgagga 1981 aagcgtctct gagtgtagca gggccaggaa aaccccagga agaagaccag cccttgcctg 2041 cccgacggct ctcctctgac tgcagcgtca ctcaggaaag gaaacagatt cattgtctgt 2101 ctgaggatga gttaagttct tctacaagtt caactgataa gtcagatggg gattacgggg 2161 aagggaaagg tcagacaaat gaaattaatg ccttgGTACA ATTGATGACT CAGACCCTGA 2221 AACTGGATTC TAAAGAGAGC TGTGAAGATG TCCCGGTAGC AAACCCAGTG TCAGAATTCA 2281 AACTTCATCG GAAATATCGG GACACACTGA TACTTCATGG GAAGGTTGCA GAAGAGGCAG 2341 AGGAAATCCA TTTTAAAGAG CTACCTTCAG CTATTATGCC AGGTTCTGAA AAGATCAGGA 2401 GACTAGTTGA AGTCTTGAGA ACTGATGTAA TTCGTGGCCT GGGAGTTCAG CTTTTAGAGC 2461 AGGTGTATGA TCTTTTGGAG GAGGAGGATG AATTTGATAG AGAGGTACGT TTGCGGGAGC 2521 ACATGGGTGA AAAGTATACA ACTTACAGTG TGAAAGCTCG CCAGTTGAAA TTTTTTGAAG 2581 AAAACATGAA TTTTTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2641 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2701 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAAACGA CAAGCGCAAC 2761 CCGACTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt