Construct: ORF TRCN0000489189

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020697.1_s317c1
DNA Barcode:
GAAACGACAAGCGCAACCCGACTA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
NEK4 (6787)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489189
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6787 NEK4 NIMA related kinase 4 NM_003157.6 99.8% 99.8% 312T>G;468A>G;673C>G
2 human 6787 NEK4 NIMA related kinase 4 XM_011534039.3 96.5% 94.3% (many diffs)
3 human 6787 NEK4 NIMA related kinase 4 NM_001348412.2 94.4% 94.4% (many diffs)
4 human 6787 NEK4 NIMA related kinase 4 XM_017007085.1 94.2% 91.4% (many diffs)
5 human 6787 NEK4 NIMA related kinase 4 NM_001348413.2 92% 90.8% (many diffs)
6 human 6787 NEK4 NIMA related kinase 4 NM_001348414.2 90.1% 90.1% (many diffs)
7 human 6787 NEK4 NIMA related kinase 4 NM_001193533.2 89.3% 89.2% 89_90ins267;201A>G;406C>G
8 human 6787 NEK4 NIMA related kinase 4 XM_017007086.1 89% 86.2% (many diffs)
9 human 6787 NEK4 NIMA related kinase 4 XM_011534040.3 79.5% 79.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2595
ORF length:
2523
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcccctg gccgcctact gctacctgcg ggtcgtgggc aaggggagct
121atggagaggt gacgcttgtg aagcaccggc gggacggcaa gcagtatgtc atcaaaaaac
181tgaacctccg aaatgcctct agccgagagc ggcgagctgc tgaacaggaa gcccagctct
241tgtctcagtt gaagcatccc aacattgtca cctacaagga gtcatgggaa ggaggagatg
301gtctgctcta cattgtcatg ggcttctgtg aaggaggtga tttgtaccga aagctcaagg
361agcagaaagg gcagcttctg ccggagaatc aggtggtaga gtggtttgta cagatcgcca
421tggctttgca gtatttacat gaaaaacaca tccttcatcg agatctgaaa actcaaaatg
481tcttcctaac aagaacaaac atcatcaaag taggggacct aggaattgcc cgagtgttgg
541agaaccactg tgacatggct agcaccctca ttggcacacc ctactacatg agccctgaat
601tgttctcaaa caaaccctac aactataagt ctgatgtttg ggctctagga tgctgtgtct
661atgaaatggc caccttgaag catgctttca atgcaaaaga tatgaattct ttagtttatc
721ggattattga aggaaagctg ccagcaatgc caagagatta cagcccagag ctggcagaac
781tgataagaac aatgctgagc aaaaggcctg aagaaaggcc gtctgtgagg agcatcctga
841ggcagcctta tataaagcgg caaatctcct tctttttgga ggccacaaag ataaaaacct
901ccaaaaataa cattaaaaat ggtgactctc aatccaagcc ttttgctaca gtggtttctg
961gagaggcaga atcaaatcat gaagtaatcc acccccaacc actctcttct gagggctccc
1021agacatatat aatgggtgaa ggcaaatgtt tgtcccagga gaaacccagg gcctctggtc
1081tcttgaagtc acctgccagt ctgaaagccc atacctgcaa acaggacttg agcaatacca
1141cagaactagc cacaatcagt agcgtaaata ttgacatctt acctgcaaaa gggagggatt
1201cagtgagtga tggctttgtt caggagaatc agccaagata tttggatgcc tctaatgagt
1261taggaggtat atgcagtatt tctcaagtgg aagaggagat gctgcaggac aacactaaat
1321ccagtgccca gcctgaaaac ctgattccca tgtggtcctc tgacattgtc actggggaaa
1381agaatgaacc agtgaagcct ctgcagcccc taatcaaaga acaaaagcca aaggaccaga
1441gtcttgccct gtcgcccaag ctggagtgca gtggcacaat cttggctcac agcaacctcc
1501gcctcctggg ttcaagtgat tctccagcct cagcctcccg agtagctggg attacaggcg
1561tgtgccacca cgcccaggat caagttgctg gtgaatgtat tatagaaaaa cagggcagaa
1621tccacccaga tttacagcca cacaactctg ggtctgaacc ttccctgtct cgacagcgac
1681ggcaaaagag gagagaacag actgagcaca gaggggaaaa gagacaggtc cgcagagatc
1741tctttgcttt ccaagagtcg cctcctcgat ttttgccttc tcatcccatt gttgggaaag
1801tggatgtcac atcaacacaa aaagaggctg aaaaccaacg tagagtggtc actgggtctg
1861tgagcagttc aaggagcagt gagatgtcat catcaaagga tcgaccatta tcagccagag
1921agaggaggcg actaaagcag tcacaggaag aaatgtcctc ttcaggccct tcagtgagga
1981aagcgtctct gagtgtagca gggccaggaa aaccccagga agaagaccag cccttgcctg
2041cccgacggct ctcctctgac tgcagcgtca ctcaggaaag gaaacagatt cattgtctgt
2101ctgaggatga gttaagttct tctacaagtt caactgataa gtcagatggg gattacgggg
2161aagggaaagg tcagacaaat gaaattaatg ccttgGTACA ATTGATGACT CAGACCCTGA
2221AACTGGATTC TAAAGAGAGC TGTGAAGATG TCCCGGTAGC AAACCCAGTG TCAGAATTCA
2281AACTTCATCG GAAATATCGG GACACACTGA TACTTCATGG GAAGGTTGCA GAAGAGGCAG
2341AGGAAATCCA TTTTAAAGAG CTACCTTCAG CTATTATGCC AGGTTCTGAA AAGATCAGGA
2401GACTAGTTGA AGTCTTGAGA ACTGATGTAA TTCGTGGCCT GGGAGTTCAG CTTTTAGAGC
2461AGGTGTATGA TCTTTTGGAG GAGGAGGATG AATTTGATAG AGAGGTACGT TTGCGGGAGC
2521ACATGGGTGA AAAGTATACA ACTTACAGTG TGAAAGCTCG CCAGTTGAAA TTTTTTGAAG
2581AAAACATGAA TTTTTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
2641TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
2701GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAAACGA CAAGCGCAAC
2761CCGACTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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