Construct: ORF TRCN0000489207
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021754.1_s317c1
- DNA Barcode:
- GATACCGTAGCAGTACGCCACGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC27A1 (376497)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489207
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 376497 | SLC27A1 | solute carrier family 27 me... | NM_198580.3 | 100% | 100% | |
| 2 | human | 376497 | SLC27A1 | solute carrier family 27 me... | XM_011528003.2 | 100% | 100% | |
| 3 | human | 376497 | SLC27A1 | solute carrier family 27 me... | XM_011528000.1 | 98.1% | 98.1% | 996_1031del |
| 4 | human | 376497 | SLC27A1 | solute carrier family 27 me... | XM_011528001.2 | 98.1% | 98.1% | 996_1031del |
| 5 | human | 376497 | SLC27A1 | solute carrier family 27 me... | XM_011528002.2 | 98.1% | 98.1% | 996_1031del |
| 6 | human | 376497 | SLC27A1 | solute carrier family 27 me... | XM_017026781.1 | 98.1% | 98.1% | 996_1031del |
| 7 | human | 376497 | SLC27A1 | solute carrier family 27 me... | XR_001753681.1 | 52.2% | 1_37del;827_828ins92;1884_3443del | |
| 8 | human | 376497 | SLC27A1 | solute carrier family 27 me... | XR_001753680.1 | 51.6% | (many diffs) | |
| 9 | mouse | 26457 | Slc27a1 | solute carrier family 27 (f... | NM_011977.3 | 84.5% | 89.4% | (many diffs) |
| 10 | mouse | 26457 | Slc27a1 | solute carrier family 27 (f... | XM_006509673.2 | 84.5% | 89.4% | (many diffs) |
| 11 | mouse | 26457 | Slc27a1 | solute carrier family 27 (f... | XM_006509674.3 | 84.5% | 89.4% | (many diffs) |
| 12 | mouse | 26457 | Slc27a1 | solute carrier family 27 (f... | XM_006509675.3 | 84.5% | 89.4% | (many diffs) |
| 13 | mouse | 26457 | Slc27a1 | solute carrier family 27 (f... | XM_006509676.3 | 84.5% | 89.4% | (many diffs) |
| 14 | mouse | 26457 | Slc27a1 | solute carrier family 27 (f... | XM_006509671.3 | 83.1% | 87.9% | (many diffs) |
| 15 | mouse | 26457 | Slc27a1 | solute carrier family 27 (f... | XM_006509677.1 | 60.9% | 64% | (many diffs) |
| 16 | mouse | 26457 | Slc27a1 | solute carrier family 27 (f... | XM_006509678.3 | 60.9% | 64% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2007
- ORF length:
- 1938
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcgggctccg ggtgcgggcg cggcctcggt ggtctcgctg gcgctgttgt 121 ggctgctggg gctgccgtgg acctggagcg cggcagcggc gctcggcgtg tacgtgggca 181 gcggcggctg gcgcttcctg cgcatcgtct gcaagaccgc gaggcgagac ctcttcggtc 241 tctctgtgct gatccgcgtg cgcctggagc tgcggcggca ccagcgtgcc ggccacacca 301 tcccgcgcat ctttcaggcg gtagtgcagc gacagcccga gcgcctggcg ctggtggatg 361 ccgggaccgg cgagtgctgg acctttgcgc agctggacgc ctactccaat gcggtagcca 421 acctcttccg ccagctgggc ttcgcgccgg gcgacgtggt ggccatcttc ctggagggcc 481 ggccggagtt cgtggggctg tggctgggcc tggccaaggc gggcatggag gccgcgctgc 541 tcaacgtgaa cctgcggcgc gagcccctgg ccttctgcct gggcacctcg ggcgctaagg 601 ccctgatctt tggaggagaa atggtggcgg cggtggccga agtgagcggg catctgggga 661 aaagtttgat caagttctgc tctggagact tggggcccga gggcatcttg ccggacaccc 721 acctcctgga cccgctgctg aaggaggcct ctactgcccc cttggcacag atccccagca 781 agggcatgga cgatcgtctt ttctacatct acacgtcggg gaccaccggg ctgcccaagg 841 ctgccattgt cgtgcacagc aggtactacc gcatggcagc cttcggccac cacgcctacc 901 gcatgcaggc ggctgacgtg ctctatgact gcctgcccct gtaccactcg gcaggaaaca 961 tcatcggcgt ggggcagtgt ctcatctatg ggctgacagt cgtcctccgc aagaaattct 1021 cggccagccg cttctgggac gactgcatca agtacaactg cacggtggtt cagtacatcg 1081 gggagatctg ccgctacctg ctgaagcagc cggtgcgcga ggcggagagg cgacaccgcg 1141 tgcgcctggc ggtggggaac gggctgcgtc ctgccatctg ggaggagttc acggagcgct 1201 tcggcgtacg ccaaatcggg gagttctacg gcgccaccga gtgcaactgc agcattgcca 1261 acatggacgg caaggtcggc tcctgtggtt tcaacagccg catcctgccc cacgtgtacc 1321 ccatccggct ggtgaaggtc aatgaggaca caatggagct gctgcgggat gcccagggcc 1381 tctgcatccc ctgccaggcc ggggagcctg gcctccttgt gggtcagatc aaccaacagg 1441 acccgctgcg ccgcttcgat ggctatgtca gcgagagcgc caccagcaag aagatcgccc 1501 acagcgtctt cagcaagggc gacagcgcct acctctcagg tgacgtgcta gtgatggatg 1561 agctgggcta catgtacttc cgggaccgta gcggggacac cttccgctgg cgaggggaga 1621 acgtctccac caccgaggtg gagggcgtgc tgagccgcct gctgggccag acagacgtgg 1681 CCGTCTATGG GGTGGCTGTT CCAGGAGTGG AGGGTAAGGC AGGGATGGCG GCCGTCGCAG 1741 ACCCCCACAG CCTGCTGGAC CCCAACGCGA TATACCAGGA GCTGCAGAAG GTGCTGGCAC 1801 CCTATGCCCG GCCCATCTTC CTGCGCCTCC TGCCCCAGGT GGACACCACA GGCACCTTCA 1861 AGATCCAGAA GACGAGGCTG CAGCGAGAGG GCTTTGACCC ACGCCAGACC TCAGACCGGC 1921 TCTTCTTCCT GGACCTGAAG CAGGGCCACT ACCTGCCCTT AAATGAGGCA GTCTACACTC 1981 GCATCTGCTC GGGCGCCTTC GCCCTCTAGA ACCCAGCTTT CTTGTACAAA GTGGTTGATA 2041 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2101 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGATAC 2161 CGTAGCAGTA CGCCACGATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2221 tgaaagatt