Construct: ORF TRCN0000489207

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021754.1_s317c1
DNA Barcode:
GATACCGTAGCAGTACGCCACGAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
SLC27A1 (376497)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489207
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 376497 SLC27A1 solute carrier family 27 me... NM_198580.3 100% 100%
2 human 376497 SLC27A1 solute carrier family 27 me... XM_011528003.2 100% 100%
3 human 376497 SLC27A1 solute carrier family 27 me... XM_011528000.1 98.1% 98.1% 996_1031del
4 human 376497 SLC27A1 solute carrier family 27 me... XM_011528001.2 98.1% 98.1% 996_1031del
5 human 376497 SLC27A1 solute carrier family 27 me... XM_011528002.2 98.1% 98.1% 996_1031del
6 human 376497 SLC27A1 solute carrier family 27 me... XM_017026781.1 98.1% 98.1% 996_1031del
7 human 376497 SLC27A1 solute carrier family 27 me... XR_001753681.1 52.2% 1_37del;827_828ins92;1884_3443del
8 human 376497 SLC27A1 solute carrier family 27 me... XR_001753680.1 51.6% (many diffs)
9 mouse 26457 Slc27a1 solute carrier family 27 (f... NM_011977.3 84.5% 89.4% (many diffs)
10 mouse 26457 Slc27a1 solute carrier family 27 (f... XM_006509673.2 84.5% 89.4% (many diffs)
11 mouse 26457 Slc27a1 solute carrier family 27 (f... XM_006509674.3 84.5% 89.4% (many diffs)
12 mouse 26457 Slc27a1 solute carrier family 27 (f... XM_006509675.3 84.5% 89.4% (many diffs)
13 mouse 26457 Slc27a1 solute carrier family 27 (f... XM_006509676.3 84.5% 89.4% (many diffs)
14 mouse 26457 Slc27a1 solute carrier family 27 (f... XM_006509671.3 83.1% 87.9% (many diffs)
15 mouse 26457 Slc27a1 solute carrier family 27 (f... XM_006509677.1 60.9% 64% (many diffs)
16 mouse 26457 Slc27a1 solute carrier family 27 (f... XM_006509678.3 60.9% 64% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2007
ORF length:
1938
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gcgggctccg ggtgcgggcg cggcctcggt ggtctcgctg gcgctgttgt
121ggctgctggg gctgccgtgg acctggagcg cggcagcggc gctcggcgtg tacgtgggca
181gcggcggctg gcgcttcctg cgcatcgtct gcaagaccgc gaggcgagac ctcttcggtc
241tctctgtgct gatccgcgtg cgcctggagc tgcggcggca ccagcgtgcc ggccacacca
301tcccgcgcat ctttcaggcg gtagtgcagc gacagcccga gcgcctggcg ctggtggatg
361ccgggaccgg cgagtgctgg acctttgcgc agctggacgc ctactccaat gcggtagcca
421acctcttccg ccagctgggc ttcgcgccgg gcgacgtggt ggccatcttc ctggagggcc
481ggccggagtt cgtggggctg tggctgggcc tggccaaggc gggcatggag gccgcgctgc
541tcaacgtgaa cctgcggcgc gagcccctgg ccttctgcct gggcacctcg ggcgctaagg
601ccctgatctt tggaggagaa atggtggcgg cggtggccga agtgagcggg catctgggga
661aaagtttgat caagttctgc tctggagact tggggcccga gggcatcttg ccggacaccc
721acctcctgga cccgctgctg aaggaggcct ctactgcccc cttggcacag atccccagca
781agggcatgga cgatcgtctt ttctacatct acacgtcggg gaccaccggg ctgcccaagg
841ctgccattgt cgtgcacagc aggtactacc gcatggcagc cttcggccac cacgcctacc
901gcatgcaggc ggctgacgtg ctctatgact gcctgcccct gtaccactcg gcaggaaaca
961tcatcggcgt ggggcagtgt ctcatctatg ggctgacagt cgtcctccgc aagaaattct
1021cggccagccg cttctgggac gactgcatca agtacaactg cacggtggtt cagtacatcg
1081gggagatctg ccgctacctg ctgaagcagc cggtgcgcga ggcggagagg cgacaccgcg
1141tgcgcctggc ggtggggaac gggctgcgtc ctgccatctg ggaggagttc acggagcgct
1201tcggcgtacg ccaaatcggg gagttctacg gcgccaccga gtgcaactgc agcattgcca
1261acatggacgg caaggtcggc tcctgtggtt tcaacagccg catcctgccc cacgtgtacc
1321ccatccggct ggtgaaggtc aatgaggaca caatggagct gctgcgggat gcccagggcc
1381tctgcatccc ctgccaggcc ggggagcctg gcctccttgt gggtcagatc aaccaacagg
1441acccgctgcg ccgcttcgat ggctatgtca gcgagagcgc caccagcaag aagatcgccc
1501acagcgtctt cagcaagggc gacagcgcct acctctcagg tgacgtgcta gtgatggatg
1561agctgggcta catgtacttc cgggaccgta gcggggacac cttccgctgg cgaggggaga
1621acgtctccac caccgaggtg gagggcgtgc tgagccgcct gctgggccag acagacgtgg
1681CCGTCTATGG GGTGGCTGTT CCAGGAGTGG AGGGTAAGGC AGGGATGGCG GCCGTCGCAG
1741ACCCCCACAG CCTGCTGGAC CCCAACGCGA TATACCAGGA GCTGCAGAAG GTGCTGGCAC
1801CCTATGCCCG GCCCATCTTC CTGCGCCTCC TGCCCCAGGT GGACACCACA GGCACCTTCA
1861AGATCCAGAA GACGAGGCTG CAGCGAGAGG GCTTTGACCC ACGCCAGACC TCAGACCGGC
1921TCTTCTTCCT GGACCTGAAG CAGGGCCACT ACCTGCCCTT AAATGAGGCA GTCTACACTC
1981GCATCTGCTC GGGCGCCTTC GCCCTCTAGA ACCCAGCTTT CTTGTACAAA GTGGTTGATA
2041TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
2101CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGATAC
2161CGTAGCAGTA CGCCACGATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2221tgaaagatt

Download FASTA (ORF) (Full)

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