Construct: ORF TRCN0000489248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020237.1_s317c1
- DNA Barcode:
- TTGAACAGTACCCTATCAGCCCTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKCD (5580)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489248
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5580 | PRKCD | protein kinase C delta | NM_001316327.2 | 99.8% | 100% | 1441C>T;1782C>G;1857T>C |
| 2 | human | 5580 | PRKCD | protein kinase C delta | NM_001354679.2 | 99.8% | 100% | 1441C>T;1782C>G;1857T>C |
| 3 | human | 5580 | PRKCD | protein kinase C delta | NM_001354680.2 | 99.8% | 100% | 1441C>T;1782C>G;1857T>C |
| 4 | human | 5580 | PRKCD | protein kinase C delta | NM_006254.4 | 99.8% | 100% | 1441C>T;1782C>G;1857T>C |
| 5 | human | 5580 | PRKCD | protein kinase C delta | NM_212539.2 | 99.8% | 100% | 1441C>T;1782C>G;1857T>C |
| 6 | human | 5580 | PRKCD | protein kinase C delta | NM_001354678.2 | 97.5% | 97.6% | (many diffs) |
| 7 | human | 5580 | PRKCD | protein kinase C delta | NM_001354676.2 | 97.1% | 97.2% | (many diffs) |
| 8 | human | 5580 | PRKCD | protein kinase C delta | XR_002959550.1 | 75.3% | (many diffs) | |
| 9 | mouse | 18753 | Prkcd | protein kinase C, delta | NM_011103.3 | 86.8% | 90.3% | (many diffs) |
| 10 | mouse | 18753 | Prkcd | protein kinase C, delta | NM_001310682.1 | 83.7% | 87% | (many diffs) |
| 11 | mouse | 18753 | Prkcd | protein kinase C, delta | XM_006518695.2 | 83.7% | 87% | (many diffs) |
| 12 | mouse | 18753 | Prkcd | protein kinase C, delta | XM_017315918.1 | 83.7% | 87% | (many diffs) |
| 13 | mouse | 18753 | Prkcd | protein kinase C, delta | XM_006518698.3 | 83.1% | 85% | (many diffs) |
| 14 | mouse | 18753 | Prkcd | protein kinase C, delta | XM_006518697.3 | 80.2% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2100
- ORF length:
- 2028
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgccg ttcctgcgca tcgccttcaa ctcctatgag ctgggctccc 121 tgcaggccga ggacgaggcg aaccagccct tctgtgccgt gaagatgaag gaggcgctca 181 gcacagagcg tgggaaaaca ctggtgcaga agaagccgac catgtatcct gagtggaagt 241 cgacgttcga tgcccacatc tatgaggggc gcgtcatcca gattgtgcta atgcgggcag 301 cagaggagcc agtgtctgag gtgaccgtgg gtgtgtcggt gctggccgag cgctgcaaga 361 agaacaatgg caaggctgag ttctggctgg acctgcagcc tcaggccaag gtgttgatgt 421 ctgttcagta tttcctggag gacgtggatt gcaaacagtc tatgcgcagt gaggacgagg 481 ccaagttccc aacgatgaac cgccgcggag ccatcaaaca ggccaaaatc cactacatca 541 agaaccatga gtttatcgcc accttctttg ggcaacccac cttctgttct gtgtgcaaag 601 actttgtctg gggcctcaac aagcaaggct acaaatgcag gcaatgtaac gctgccatcc 661 acaagaaatg catcgacaag atcatcggca gatgcactgg caccgcggcc aacagccggg 721 acactatatt ccagaaagaa cgcttcaaca tcgacatgcc gcaccgcttc aaggttcaca 781 actacatgag ccccaccttc tgtgaccact gcggcagcct gctctgggga ctggtgaagc 841 agggattaaa gtgtgaagac tgcggcatga atgtgcacca taaatgccgg gagaaggtgg 901 ccaacctctg cggcatcaac cagaagcttt tggctgaggc cttgaaccaa gtcacccaga 961 gagcctcccg gagatcagac tcagcctcct cagagcctgt tgggatatat cagggtttcg 1021 agaagaagac cggagttgct ggggaggaca tgcaagacaa cagtgggacc tacggcaaga 1081 tctgggaggg cagcagcaag tgcaacatca acaacttcat cttccacaag gtcctgggca 1141 aaggcagctt cgggaaggtg ctgcttggag agctgaaggg cagaggagag tactttgcca 1201 tcaaggccct caagaaggat gtggtcctga tcgacgacga cgtggagtgc accatggttg 1261 agaagcgggt gctgacactt gccgcagaga atccctttct cacccacctc atctgcacct 1321 tccagaccaa ggaccacctg ttctttgtga tggagttcct caacgggggg gacctgatgt 1381 accacatcca ggacaaaggc cgctttgaac tctaccgtgc cacgttttat gccgctgaga 1441 taatgtgtgg actgcagttt ctacacagca agggcatcat ttacagggac ctcaaactgg 1501 acaatgtgct gttggaccgg gatggccaca tcaagattgc cgactttggg atgtgcaaag 1561 agaacatatt cggggagagc cgggccagca ccttctgcgg cacccctgac tatatcgccc 1621 ctgagatcct acagggcctg aagtacacat tctctgtgga ctggtggtct ttcggggtcc 1681 ttctgtacga gatgctcatt ggccagtccc ccTTCCATGG TGATGATGAG GATGAACTCT 1741 TCGAGTCCAT CCGTGTGGAC ACGCCACATT ATCCCCGCTG GATCACCAAG GAGTCCAAGG 1801 ACATCCTGGA GAAGCTCTTT GAAAGGGAAC CAACCAAGAG GCTGGGAGTG ACGGGAAACA 1861 TCAAAATCCA CCCCTTCTTC AAGACCATAA ACTGGACTCT GCTGGAAAAG CGGAGGTTGG 1921 AGCCACCCTT CAGGCCCAAA GTGAAGTCAC CCAGAGACTA CAGTAACTTT GACCAGGAGT 1981 TCCTGAACGA GAAGGCGCGC CTCTCCTACA GCGACAAGAA CCTCATCGAC TCCATGGACC 2041 AGTCTGCATT CGCTGGCTTC TCCTTTGTGA ACCCCAAATT CGAGCACCTC CTGGAAGATT 2101 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2161 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2221 GGCTTTATAT ATCTTGTGGA AAGGACGATT GAACAGTACC CTATCAGCCC TGACGCGTTA 2281 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt