Construct: ORF TRCN0000489299
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019908.1_s317c1
- DNA Barcode:
- TGTGGATGATTATCAGCCCCGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAT10 (55226)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489299
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55226 | NAT10 | N-acetyltransferase 10 | NM_024662.3 | 99.9% | 99.8% | 1381T>C;1686C>T;3075_3076insG |
| 2 | human | 55226 | NAT10 | N-acetyltransferase 10 | NM_001144030.2 | 92.8% | 92.7% | (many diffs) |
| 3 | mouse | 98956 | Nat10 | N-acetyltransferase 10 | NM_153126.2 | 88.7% | 95.4% | (many diffs) |
| 4 | mouse | 98956 | Nat10 | N-acetyltransferase 10 | XM_006500455.3 | 76.6% | 80.6% | (many diffs) |
| 5 | mouse | 98956 | Nat10 | N-acetyltransferase 10 | XR_374547.3 | 47.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3147
- ORF length:
- 3078
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcatcggaaa aaggtggata accgaatccg gattctcatt gagaatggag 121 tagctgagcg gcaaagatct ctctttgttg tagttgggga tcgaggaaaa gatcaggtgg 181 taatacttca tcacatgtta tccaaagcaa ctgtgaaggc tcggccttca gtgctgtggt 241 gttataagaa agagctgggg tttagcagtc accggaagaa aagaatgcga cagctgcaga 301 agaaaataaa gaatggaaca ctgaacataa agcaggacga cccctttgaa ctcttcatag 361 cagccacaaa cattcgctac tgctactaca acgagaccca caagatcctg ggcaatacct 421 tcggcatgtg tgtgctgcag gattttgaag ccttaactcc aaacttgctg gccaggactg 481 tagaaacagt ggaaggtggt gggctagtgg tcatcctcct acggaccatg aactcactca 541 agcaattgta cacagtgact atggatgtgc attccaggta cagaactgag gcccatcagg 601 atgtggtggg aagatttaat gaaaggttta ttctgtctct ggcctcttgt aagaagtgtc 661 tcgtcattga tgaccagctc aacatcctgc ccatctcctc ccacgttgcc accatggagg 721 ccctgcctcc ccagactccg gatgagagtc ttggtccttc tgatctggag ctgagggagt 781 tgaaggagag cttgcaggac acccagcctg tgggtgtgtt ggtggactgc tgtaagactc 841 tagaccaggc caaagctgtc ttgaaattta tcgagggcat ctctgaaaag accctgagga 901 gtactgttgc actcacagct gctcgaggac ggggaaaatc tgcagccctg ggattggcga 961 ttgctggggc ggtggcattt gggtactcca atatctttgt tacctcccca agccctgata 1021 acctccatac tctgtttgaa tttgtattta aaggatttga tgctctgcaa tatcaggaac 1081 atctggatta tgagattatc cagtctctaa atcctgaatt taacaaagca gtgatcagag 1141 tgaatgtatt tcgagaacac aggcagacta ttcagtatat acatcctgca gatgctgtga 1201 agctgggcca ggctgaacta gttgtgattg atgaagctgc cgccatcccc ctccccttgg 1261 tgaagagcct acttggcccc taccttgttt tcatggcatc caccatcaat ggctatgagg 1321 gcactggccg gtcactgtcc ctcaagctaa ttcagcagct ccgtcaacag agcgcccaga 1381 gccaggtcag caccactgct gagaataaga ccacgacgac agccagattg gcatcagcgc 1441 ggacactgca tgaggtttcc ctccaggagt caatccgata cgcccctggg gatgcagtgg 1501 agaagtggct gaatgacttg ctgtgcctgg attgcctcaa catcactcgg atagtctcag 1561 gctgcccctt gcctgaagct tgtgaactgt actatgttaa tagagatacc ctcttttgct 1621 accacaaggc ctctgaagtt ttcctccaac ggcttatggc cctctacgtg gcttctcact 1681 acaagaactc tcccaatgat ctccagatgc tctccgatgc acctgctcac catctcttct 1741 gccttctgcc tcctgtgccc cccacccaga atgcccttcc agaagtgctt gctgttatcc 1801 aggtgtgcct tgaaggggag atttctcgcc agtccatctt gaacagtctg tctcgaggca 1861 agaaggcttc aggggacctg attccatgga cagtgtcaga acagttccaa gatccagact 1921 ttggtggtct gtctggtgga agggtcgttc gcattgctgt tcacccagat tatcaaggga 1981 tgggctatgg cagccgtgct ctgcagctgc tgcagatgta ctatgaaggc aggtttcctt 2041 gtctggagga aaaggtcctt gagacaccac aggaaattca caccgtaagc agcgaggctg 2101 tcagcttgtt ggaagaggtc atcactcccc ggaaggacct gcctccttta ctcctcaaat 2161 tgaatgagag gcctgccgaa cgcctggatt acctgggtgt ttcctatggc ttgaccccca 2221 ggctcctcaa gttctggaaa cgagctggat ttgttcctgt ttatctgaga cagaccccga 2281 atgacctgac cggagagcac tcgtgcatca tgctgaagac gctcactgat gaggatgagg 2341 ctgaccaggg aggctggctt gcagccttct ggaaagattt ccgacggcgg ttcctagcct 2401 tgctctccta ccagttcagt accttctctc cttccctggc tctgaacatc attcagaaca 2461 ggaacatggg gaagccagcc cagcctgccc tgagccggga ggagctggaa gcactcttcc 2521 tcccctatga cctgaagcgg ctggagatgt attcacggaa tatggtggac tatcacctca 2581 tcatggacat gatcccggcc atctctcgca tctatttcct gaaccagctg ggggacctgg 2641 ccctgtctgc ggctcagtcg gctcttctct tggggattgg cctgcagcat aagtctgtgg 2701 accagctgga aaaggagatt gagctgccct cgggccagtt gatgggactt ttcaaccgga 2761 TCATCCGCAA AGTTGTGAAG CTATTTAATG AAGTTCAGGA AAAGGCCATT GAGGAGCAGA 2821 TGGTGGCAGC GAAGGATGTG GTCATGGAGC CCACGATGAA GACCCTCAGT GACGACCTAG 2881 ATGAAGCAGC AAAGGAATTT CAGGAGAAAC ACAAGAAGGA AGTAGGGAAG CTGAAGAGCA 2941 TGGACCTCTC TGAATACATA ATCCGTGGGG ACGATGAAGA GTGGAATGAA GTTTTGAACA 3001 AAGCTGGGCC GAACGCCTCG ATCATCAGCC TGAAAAGTGA CAAGAAAAGG AAGTTAGAGG 3061 CCAAACAAGA ACCCAAACAG AGCAAGAAGT TGAAGAACAG AGAGACAAAG AACAAAAAAG 3121 ATATGAAACT GAAGCGGAAG AAAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3181 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3241 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GTGGATGATT 3301 ATCAGCCCCG TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3361 att