Construct: ORF TRCN0000489360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020696.1_s317c1
- DNA Barcode:
- GATGATTGTCGATGCACTCTCCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NRBP2 (340371)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489360
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340371 | NRBP2 | nuclear receptor binding pr... | NM_178564.4 | 51.4% | 51.4% | 1_729del |
| 2 | human | 340371 | NRBP2 | nuclear receptor binding pr... | XM_017013382.1 | 44.9% | 38% | 1_729del;1382_1472del;1595_1722del |
| 3 | human | 340371 | NRBP2 | nuclear receptor binding pr... | XM_017013381.2 | 43.5% | 40.9% | (many diffs) |
| 4 | human | 340371 | NRBP2 | nuclear receptor binding pr... | XM_017013380.1 | 42.5% | 36% | 1_729del;1382_1472del;1595_1818del |
| 5 | human | 340371 | NRBP2 | nuclear receptor binding pr... | XM_017013383.1 | 41.5% | 39% | 1_729del;1381_1382ins56;1448_1671del |
| 6 | human | 340371 | NRBP2 | nuclear receptor binding pr... | XR_001745528.1 | 39.8% | 1_869del;1522_1612del;1735_1942del | |
| 7 | human | 340371 | NRBP2 | nuclear receptor binding pr... | XM_017013378.1 | 39% | 33% | 1_729del;1382_1472del;1595_1983del |
| 8 | human | 340371 | NRBP2 | nuclear receptor binding pr... | XR_001745529.1 | 38.5% | 1_869del;1522_1612del;1735_2010del | |
| 9 | human | 340371 | NRBP2 | nuclear receptor binding pr... | XM_017013379.1 | 37.9% | 35.6% | 1_729del;1381_1382ins56;1448_1836del |
| 10 | mouse | 223649 | Nrbp2 | nuclear receptor binding pr... | NM_144847.1 | 87% | 94.1% | (many diffs) |
| 11 | mouse | 223649 | Nrbp2 | nuclear receptor binding pr... | XM_006520772.3 | 45% | 48.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 846
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtgtgcg ctggagatgg ctgtactgga aatccagacc aatggggaca 121 cccgggtcac agaggaggcc attgctcgcg ccaggcactc gctgagtgac cccaacatgc 181 gggagttcat cctttgctgc ctggcccggg accctgcccg ccggccctct gcccacagcc 241 tcctcttcca ccgcgtgctc ttcgaggtgc actcgctgaa gctcctggca gcccactgct 301 tcatccagca ccagtacctc atgcctgaga atgtggtgga ggagaagacc aaggccatgg 361 acctgcacgc ggtcttggcg gagcttcccc ggccccgcag gcccccgctg cagtggcggt 421 actcggaagt ctccttcatg gagctggaca aattcctgGA GGATGTCAGG AATGGAATCT 481 ACCCACTGAT GAACTTTGCA GCCACTCGAC CCCTGGGGCT GCCCCGTGTG CTGGCCCCAC 541 CCCCGGAGGA GGTCCAAAAG GCCAAGACCC CGACGCCAGA GCCCTTTGAC TCTGAGACCA 601 GAAAGGTCAT CCAGATGCAG TGCAACCTGG AGAGAAGCGA GGACAAGGCG CGCTGGCATC 661 TCACTCTGCT TCTGGTGCTG GAAGACCGGC TGCACCGGCA GCTGACCTAC GACCTGCTCC 721 CAACGGACAG CGCCCAGGAC CTCGCCTCGG AGCTCGTGCA CTATGGCTTC CTCCACGAGG 781 ACGACCGGAT GAAGCTGGCC GCCTTCCTGG AGAGCACCTT CCTCAAGTAC CGTGGGACCC 841 AGGCCTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGATGAT TGTCGATGCA CTCTCCTCAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt