Construct: ORF TRCN0000489362
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020463.1_s317c1
- DNA Barcode:
- TAACTTCACCGACTGAACCTGCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK26 (51765)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489362
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_016542.4 | 100% | 100% | |
| 2 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_001042452.2 | 85% | 85% | 597_598ins186 |
| 3 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_001042453.2 | 81.4% | 81.4% | 40_41ins231 |
| 4 | human | 51765 | STK26 | serine/threonine kinase 26 | XM_011531349.1 | 76.4% | 76.4% | 0_1ins294 |
| 5 | human | 51765 | STK26 | serine/threonine kinase 26 | XM_011531350.1 | 74.8% | 71.8% | (many diffs) |
| 6 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | NM_133729.2 | 91.1% | 98% | (many diffs) |
| 7 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | NM_001313744.1 | 86.3% | 85.3% | (many diffs) |
| 8 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | XM_017318618.1 | 73.9% | 79.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1320
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcccac tcgccggtgg ctgtccaagt gcctgggatg cagaataaca 121 tagctgatcc agaagaactg ttcacaaaat tagagcgcat tgggaaaggc tcatttgggg 181 aagttttcaa aggaattgat aaccgtaccc agcaagtcgt tgctattaaa atcatagacc 241 ttgaggaagc cgaagatgaa atagaagaca ttcagcaaga aataactgtc ttgagtcaat 301 gtgacagctc atatgtaaca aaatactatg ggtcatattt aaaggggtct aaattatgga 361 taataatgga atacctgggc ggtggttcag cactggatct tcttcgagct ggtccatttg 421 atgagttcca gattgctacc atgctaaagg aaattttaaa aggtctggac tatctgcatt 481 cagaaaagaa aattcaccga gacataaaag ctgccaatgt cttgctctca gaacaaggag 541 atgttaaact tgctgatttt ggagttgctg gtcagctgac agatacacag attaaaagaa 601 atacctttgt gggaactcca ttttggatgg ctcctgaagt tattcaacag tcagcttatg 661 actcaaaagc tgacatttgg tcattgggaa ttactgctat tgaactagcc aagggagagc 721 cacctaactc cgatatgcat ccaatgagag ttctgtttct tattcccaaa aacaatcctc 781 caactcttgt tggagacttt actaagtctt ttaaggagtt tattgatgct tgcctgaaca 841 aagatccatc atttcgtcct acagcaaaag aacttctgaa acacaaattc attgtaaaaa 901 attcaaagaa gacttcttat ctgactgaac tgatagatcg ttttaagaga tggaaggcag 961 aaggacacag tgatgatgaa tctgattccg agggctctga ttcggaatct accagcaggg 1021 aaaacaatac tcaTCCTGAA TGGAGCTTTA CCACCGTACG AAAGAAGCCT GATCCAAAGA 1081 AAGTACAGAA TGGGGCAGAG CAAGATCTTG TGCAAACCCT GAGTTGTTTG TCTATGATAA 1141 TCACACCTGC ATTTGCTGAA CTTAAACAGC AGGACGAGAA TAACGCTAGC AGGAATCAGG 1201 CGATTGAAGA ACTCGAGAAA AGTATTGCTG TGGCTGAAGC CGCCTGTCCC GGCATCACAG 1261 ATAAAATGGT GAAGAAACTA ATTGAAAAAT TTCAAAAGTG TTCAGCAGAC GAATCCCCCT 1321 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGATA ACTTCACCGA CTGAACCTGC CGACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt