Construct: ORF TRCN0000489398
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020494.1_s317c1
- DNA Barcode:
- TCACAGGTGCGAGCCCAGATTTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ACVR2A (92)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489398
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92 | ACVR2A | activin A receptor type 2A | NM_001278579.2 | 99.9% | 100% | 354G>A |
| 2 | human | 92 | ACVR2A | activin A receptor type 2A | NM_001616.5 | 99.9% | 100% | 354G>A |
| 3 | human | 92 | ACVR2A | activin A receptor type 2A | NM_001278580.1 | 78.8% | 78.9% | 0_1ins324;30G>A |
| 4 | mouse | 11480 | Acvr2a | activin receptor IIA | NM_007396.4 | 94.5% | 99.6% | (many diffs) |
| 5 | mouse | 11480 | Acvr2a | activin receptor IIA | XM_006497623.3 | 93% | 98% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1611
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggagct gctgcaaagt tggcgtttgc cgtctttctt atctcctgtt 121 cttcaggtgc tatacttggt agatcagaaa ctcaggagtg tcttttcttt aatgctaatt 181 gggaaaaaga cagaaccaat caaactggtg ttgaaccgtg ttatggtgac aaagataaac 241 ggcggcattg ttttgctacc tggaagaata tttctggttc cattgaaata gtgaaacaag 301 gttgttggct ggatgatatc aactgctatg acaggactga ttgtgtagaa aaaaaagaca 361 gccctgaagt atatttttgt tgctgtgagg gcaatatgtg taatgaaaag ttttcttatt 421 ttccagagat ggaagtcaca cagcccactt caaatccagt tacacctaag ccaccctatt 481 acaacatcct gctctattcc ttggtgccac ttatgttaat tgcggggatt gtcatttgtg 541 cattttgggt gtacaggcat cacaagatgg cctaccctcc tgtacttgtt ccaactcaag 601 acccaggacc acccccacct tctccattac taggtttgaa accactgcag ttattagaag 661 tgaaagcaag gggaagattt ggttgtgtct ggaaagccca gttgcttaac gaatatgtgg 721 ctgtcaaaat atttccaata caggacaaac agtcatggca aaatgaatac gaagtctaca 781 gtttgcctgg aatgaagcat gagaacatat tacagttcat tggtgcagaa aaacgaggca 841 ccagtgttga tgtggatctt tggctgatca cagcatttca tgaaaagggt tcactatcag 901 actttcttaa ggctaatgtg gtctcttgga atgaactgtg tcatattgca gaaaccatgg 961 ctagaggatt ggcatattta catgaggata tacctggcct aaaagatggc cacaaacctg 1021 ccatatctca cagggacatc aaaagtaaaa atgtgctgtt gaaaaacaac ctgacagctt 1081 gcattgctga ctttgggttg gccttaaaat ttgaggctgg caagtctgca ggcgataccc 1141 atggacaggt tggtacccgg aggtacatgg ctccagaggt attagagggt gctataaact 1201 tccaaaggga tgcatttttg aggatagata tgtatgccat gggattagtc ctatgggaac 1261 tggcttctcg ctgtactgct gcagatGGAC CTGTAGATGA ATACATGTTG CCATTTGAGG 1321 AGGAAATTGG CCAGCATCCA TCTCTTGAAG ACATGCAGGA AGTTGTTGTG CATAAAAAAA 1381 AGAGGCCTGT TTTAAGAGAT TATTGGCAGA AACATGCTGG AATGGCAATG CTCTGTGAAA 1441 CCATTGAAGA ATGTTGGGAT CACGACGCAG AAGCCAGGTT ATCAGCTGGA TGTGTAGGTG 1501 AAAGAATTAC CCAGATGCAG AGACTAACAA ATATTATTAC CACAGAGGAC ATTGTAACAG 1561 TGGTCACAAT GGTGACAAAT GTTGACTTTC CTCCCAAAGA ATCTAGTCTA TAGGACCCAG 1621 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1681 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1741 TATCTTGTGG AAAGGACGAT CACAGGTGCG AGCCCAGATT TCAACGCGTT AAGTCgacaa 1801 tcaacctctg gattacaaaa tttgtgaaag att