Construct: ORF TRCN0000489419
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022036.1_s317c1
- DNA Barcode:
- ATGACAAGACAACCAGGTTGATTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC29A4 (222962)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489419
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222962 | SLC29A4 | solute carrier family 29 me... | NM_001040661.2 | 100% | 100% | |
| 2 | human | 222962 | SLC29A4 | solute carrier family 29 me... | NM_153247.3 | 100% | 100% | |
| 3 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_006715667.3 | 100% | 100% | |
| 4 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_011515200.2 | 100% | 100% | |
| 5 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_011515201.2 | 100% | 100% | |
| 6 | human | 222962 | SLC29A4 | solute carrier family 29 me... | NM_001300847.2 | 97.3% | 91.7% | 412_413ins37;504_505insCAGTG |
| 7 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_005249658.4 | 97.3% | 91.7% | 412_413ins37;504_505insCAGTG |
| 8 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_006715668.2 | 63.7% | 63.7% | 0_1ins576 |
| 9 | mouse | 243328 | Slc29a4 | solute carrier family 29 (n... | NM_146257.2 | 83.5% | 86.7% | (many diffs) |
| 10 | mouse | 243328 | Slc29a4 | solute carrier family 29 (n... | XM_006504706.2 | 83.5% | 86.7% | (many diffs) |
| 11 | mouse | 243328 | Slc29a4 | solute carrier family 29 (n... | XM_006504707.2 | 59.6% | 55.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1662
- ORF length:
- 1590
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggctcc gtggggagcc agcgccttga ggagcccagc gtggcaggca 121 caccagaccc gggcgtagtg atgagcttca ccttcgacag tcaccagctg gaggaggcgg 181 cggaggcggc tcagggccag ggccttaggg ccaggggcgt cccagctttc acggatacta 241 cattggacga gccagtgccc gatgaccgtt atcacgccat ctactttgcg atgctgctgg 301 ctggcgtggg cttcctgctg ccatacaaca gcttcatcac ggacgtggac tacctgcatc 361 acaagtaccc agggacctcc atcgtgtttg acatgagcct cacctacatc ttggtggcac 421 tggcagctgt cctcctgaac aacgtcctgg tggagagact gaccctgcac accaggatca 481 ccgcaggcta cctcttagcc ttgggccctc tcctttttat cagcatctgc gacgtgtggc 541 tgcagctctt ctctcgggac caggcctacg ccatcaacct ggccgctgtg ggcaccgtgg 601 ccttcggctg cacagtgcag caatccagct tctacgggta cacggggatg ctgcccaagc 661 ggtacacgca gggggtgatg accggggaga gcacggcggg cgtgatgatc tctctgagcc 721 gcatcctcac gaagctgctg ctgcccgacg agcgcgccag cacgctcatc ttcttcctgg 781 tgtcggtggc gctggagctg ctgtgtttcc tgctgcacct gttagtgcgg cgcagccgct 841 tcgtgctctt ctataccaca cggccgcgtg acagccaccg gggcaggcca ggcctgggca 901 ggggctatgg ctaccgcgtg caccacgacg ttgtcgccgg ggacgtccac ttcgagcacc 961 cagccccggc cctggccccc aacgagtccc caaaggacag cccagcccac gaggtgaccg 1021 gcagcggcgg ggcctacatg cgctttgatg tgccgcggcc aagggtccag cgcagctggc 1081 ccaccttcag agccctgtta ctgcaccgct acgtggtggc gcgggtgatc tgggccgaca 1141 tgctctccat cgccgtgacc tacttcatca cgctgtgcct gttccccggc ctcgagtctg 1201 agatccgcca ctgcatcctg ggcgagtggc tgcccatcct catcatggct gtgttcaacc 1261 tgtcagactt cgtgggcaag atcctggcag ccctgcccgt ggactggcgg ggcacccacc 1321 tgctggcctg cTCCTGCCTG CGTGTGGTCT TCATCCCCCT CTTCATCCTG TGCGTCTACC 1381 CCAGCGGCAT GCCCGCCCTC CGTCACCCCG CCTGGCCCTG CATCTTCTCA CTGCTCATGG 1441 GCATCAGCAA CGGCTACTTC GGCAGCGTGC CCATGATCCT GGCGGCAGGC AAAGTGAGCC 1501 CCAAGCAGCG GGAGCTGGCA GGGAACACCA TGACCGTGTC CTACATGTCA GGGCTGACGC 1561 TGGGGTCCGC CGTGGCCTAC TGCACCTACA GCCTCACCCG CGACGCTCAC GGCAGCTGCC 1621 TGCACGCCTC CACCGCCAAT GGTTCCATCC TCGCAGGCCT CTGAGACCCA GCTTTCTTGT 1681 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1741 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1801 GAAAGGACGA ATGACAAGAC AACCAGGTTG ATTCACGCGT TAAGTCgaca atcaacctct 1861 ggattacaaa atttgtgaaa gatt