Construct: ORF TRCN0000489486
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021731.1_s317c1
- DNA Barcode:
- AGACCGCATTTGGTGACAGGGCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PDGFRA (5156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489486
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347829.2 | 47.8% | .5% | 1_1702del;3222T>C |
| 2 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.6 | 47.8% | .5% | 1_1702del;3222T>C |
| 3 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_005265743.1 | 47.8% | .5% | 1_1702del;3222T>C |
| 4 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.4:c.2522_2527del | 47.6% | .5% | 1_1702del;2519_2520insCAGAGA;3216T>C |
| 5 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.4:c.2526_2537del | 47.5% | .5% | 1_1702del;2523_2524insGACATCATGCAT;3210T>C |
| 6 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347830.1 | 47.3% | .5% | 1_1741del;3261T>C |
| 7 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347828.2 | 46.7% | .5% | 1_1777del;3297T>C |
| 8 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347827.2 | 21.1% | .7% | (many diffs) |
| 9 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_017008281.1 | 20.9% | .7% | (many diffs) |
| 10 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_006714041.3 | 20.7% | .7% | (many diffs) |
| 11 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_001083316.2 | 42.8% | .6% | (many diffs) |
| 12 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_011058.3 | 42.8% | .6% | (many diffs) |
| 13 | mouse | 18595 | Pdgfra | platelet derived growth fac... | XM_006504261.3 | 42.8% | .6% | (many diffs) |
| 14 | mouse | 18595 | Pdgfra | platelet derived growth fac... | XM_006504262.3 | 42.8% | .6% | (many diffs) |
| 15 | mouse | 18595 | Pdgfra | platelet derived growth fac... | XM_006504263.3 | 42.8% | .6% | (many diffs) |
| 16 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_001347718.1 | 17.6% | .8% | (many diffs) |
| 17 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_001347719.1 | 17.6% | .8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 79
- ORF end:
- 154
- ORF length:
- 75
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctcaat cagcccagat ggacatgaat atatttatgt ggacccgatg cagctgcctt 121 atgactcaag atgggagttt ccaagagatg gactagtgct tggtcgggtc ttggggtctg 181 gagcgtttgg gaaggtggtt gaaggaacag cctatggatt aagccggtcc caacctgtca 241 tgaaagttgc agtgaagatg ctaaaaccca cggccagatc cagtgaaaaa caagctctca 301 tgtctgaact gaagataatg actcacctgg ggccacattt gaacattgta aacttgctgg 361 gagcctgcac caagtcaggc cccatttaca tcatcacaga gtattgcttc tatggagatt 421 tggtcaacta tttgcataag aatagggata gcttcctgag ccaccaccca gagaagccaa 481 agaaagagct ggatatcttt ggattgaacc ctgctgatga aagcacacgg agctatgtta 541 ttttatcttt tgaaaacaat ggtgactaca tggacatgaa gcaggctgat actacacagt 601 atgtccccat gctagaaagg aaagaggttt ctaaatattc cgacatccag agatcactct 661 atgatcgtcc agcctcatat aagaagaaat ctatgttaga ctcagaagtc aaaaacctcc 721 tttcagatga taactcagaa ggccttactt tattggattt gttgagcttc acctatcaag 781 ttgcccgagg aatggagttt ttggcttcaa aaaattgtgt ccaccgtgat ctggctgctc 841 gcaacgtcct cctggcacaa ggaaaaattg tgaagatctg tgactttggc ctggccagag 901 acatcatgca tgattcgaac tatgtgtcga aaggcagtac ctttctgccc gtgaagtgga 961 tggctcctga gagcatcttt gacaacctct acaccacact gagtgatgtc tggtcttatg 1021 gcattctgct ctgggagatc ttttcccttg gtggcacccc ttaccccggc atgatggtgg 1081 attctacttt ctacaataag atcaagagtg ggtaccggat ggccaagcct gaccacgcta 1141 ccagtgaagt ctacgagatc atggtgaaat gctggaacag tgagccggag aagagaccct 1201 ccttttacca cctgagtgag attgtggaga atctgctgcc tggacaatat aaaaagagtt 1261 atgaaaaaat tcacctggac ttcctgaaga gtgaccatcc tgctgtggca cgcatgcgtg 1321 tggacTCAGA CAATGCATAC ATTGGTGTCA CCTACAAAAA CGAGGAAGAC AAGCTGAAGG 1381 ACTGGGAGGG TGGTCTGGAT GAGCAGAGAC TGAGCGCTGA CAGTGGCTAC ATCATTCCTC 1441 TGCCTGACAT TGACCCTGTC CCTGAGGAGG AGGACCTGGG CAAGAGGAAC AGACACAGCT 1501 CGCAGACCTC TGAAGAGAGT GCCATTGAGA CGGGTTCCAG CAGTTCCACC TTCATCAAGA 1561 GAGAGGACGA GACCATTGAA GACATCGACA TGATGGACGA CATCGGCATA GACTCTTCAG 1621 ACCTGGTGGA AGACAGCTTC CTGTAAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1681 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1741 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGACCGCA 1801 TTTGGTGACA GGGCCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1861 aagatt