Construct: ORF TRCN0000489556
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020499.1_s317c1
- DNA Barcode:
- AAGAAAAGCCACTTGGCTTATTAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K3 (5606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489556
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_145109.3 | 100% | 100% | |
| 2 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024857.2 | 97.6% | 95.9% | (many diffs) |
| 3 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_001316332.2 | 91.6% | 91.6% | 0_1ins87 |
| 4 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_002756.4 | 91.6% | 91.6% | 0_1ins87 |
| 5 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_005256723.2 | 91.6% | 91.6% | 0_1ins87 |
| 6 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_011523959.2 | 91.6% | 91.6% | 0_1ins87 |
| 7 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024858.1 | 91.6% | 91.6% | 0_1ins87 |
| 8 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024859.1 | 91.6% | 91.6% | 0_1ins87 |
| 9 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_011523958.2 | 72.6% | 66.1% | 0_1ins87;685_686ins140;815_894del |
| 10 | mouse | 26397 | Map2k3 | mitogen-activated protein k... | NM_008928.4 | 90.6% | 96.5% | (many diffs) |
| 11 | mouse | 26397 | Map2k3 | mitogen-activated protein k... | XM_006533368.3 | 67.5% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1113
- ORF length:
- 1041
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagtcg cccgcctcga gccagcccgc cagcatgccc cagtccaaag 121 gaaaatccaa gaggaagaag gatctacgga tatcctgcat gtccaagcca cccgcaccca 181 accccacacc cccccggaac ctggactccc ggaccttcat caccattgga gacagaaact 241 ttgaggtgga ggctgatgac ttggtgacca tctcagaact gggccgtgga gcctatgggg 301 tggtagagaa ggtgcggcac gcccagagcg gcaccatcat ggccgtgaag cggatccggg 361 ccaccgtgaa ctcacaggag cagaagcggc tgctcatgga cctggacatc aacatgcgca 421 cggtcgactg tttctacact gtcaccttct acggggcact attcagagag ggagacgtgt 481 ggatctgcat ggagctcatg gacacatcct tggacaagtt ctaccggaag gtgctggata 541 aaaacatgac aattccagag gacatccttg gggagattgc tgtgtctatc gtgcgggccc 601 tggagcatct gcacagcaag ctgtcggtga tccacagaga tgtgaagccc tccaatgtcc 661 ttatcaacaa ggagggccat gtgaagatgt gtgactttgg catcagtggc tacttggtgg 721 actctgtggc caagacgatg gatgccggct gcaagcccta catggCCCCT GAGAGGATCA 781 ACCCAGAGCT GAACCAGAAG GGCTACAATG TCAAGTCCGA CGTCTGGAGC CTGGGCATCA 841 CCATGATTGA GATGGCCATC CTGCGGTTCC CTTACGAGTC CTGGGGGACC CCGTTCCAGC 901 AGCTGAAGCA GGTGGTGGAG GAGCCGTCCC CCCAGCTCCC AGCCGACCGT TTCTCCCCCG 961 AGTTTGTGGA CTTCACTGCT CAGTGCCTGA GGAAGAACCC CGCAGAGCGT ATGAGCTACC 1021 TGGAGCTGAT GGAGCACCCC TTCTTCACCT TGCACAAAAC CAAGAAGACG GACATTGCTG 1081 CCTTCGTGAA GGAGATCCTG GGAGAAGACT CATAGGACCC AGCTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 AAAGAAAAGC CACTTGGCTT ATTACACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt