Construct: ORF TRCN0000489565
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021803.1_s317c1
- DNA Barcode:
- GTACGATGCCCACACCGAACAAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BTN3A1 (11119)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489565
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | NM_007048.6 | 100% | 100% | |
| 2 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XM_005248833.2 | 98.6% | 98.4% | 916_917ins21 |
| 3 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | NM_001145008.2 | 89.8% | 89.8% | 425_426ins156 |
| 4 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XM_006714983.3 | 81.6% | 81.6% | 429_430ins282 |
| 5 | human | 10384 | BTN3A3 | butyrophilin subfamily 3 me... | NM_006994.5 | 79.3% | 74.8% | (many diffs) |
| 6 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | NM_001145009.2 | 71.9% | 66.7% | (many diffs) |
| 7 | human | 10384 | BTN3A3 | butyrophilin subfamily 3 me... | NM_197974.3 | 71.9% | 67.9% | (many diffs) |
| 8 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | NM_194441.3 | 66.5% | 66.4% | (many diffs) |
| 9 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XM_005248834.4 | 66.5% | 66.4% | (many diffs) |
| 10 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XR_001743130.1 | 63.7% | (many diffs) | |
| 11 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_001197246.2 | 58.5% | 53.8% | (many diffs) |
| 12 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_001197247.3 | 58.5% | 53.8% | (many diffs) |
| 13 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_007047.5 | 58.5% | 53.8% | (many diffs) |
| 14 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_005248827.4 | 58.5% | 53.8% | (many diffs) |
| 15 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010211.1 | 58.5% | 53.8% | (many diffs) |
| 16 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010212.1 | 58.5% | 53.8% | (many diffs) |
| 17 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_006714979.4 | 57.9% | 53.2% | (many diffs) |
| 18 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_006714980.4 | 57.9% | 53.2% | (many diffs) |
| 19 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_006714981.4 | 57.9% | 53.2% | (many diffs) |
| 20 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_011514267.3 | 57.9% | 53.2% | (many diffs) |
| 21 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_001197248.2 | 53.9% | 49.7% | (many diffs) |
| 22 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | NM_001197249.2 | 51.1% | 46.7% | (many diffs) |
| 23 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010214.2 | 51.1% | 46.7% | (many diffs) |
| 24 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_006714982.4 | 50.6% | 46.3% | (many diffs) |
| 25 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_011514269.3 | 50.6% | 46.3% | (many diffs) |
| 26 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010213.2 | 50.6% | 46.3% | (many diffs) |
| 27 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_005248831.4 | 49% | 46% | (many diffs) |
| 28 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_005248832.4 | 49% | 46% | (many diffs) |
| 29 | human | 10384 | BTN3A3 | butyrophilin subfamily 3 me... | NM_001242803.2 | 48% | 46.4% | (many diffs) |
| 30 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XR_926039.3 | 41.6% | 1_336del;1354_1646del;2169_3695del | |
| 31 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010216.2 | 41.6% | 38.9% | (many diffs) |
| 32 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_011514270.3 | 41.5% | 37.2% | (many diffs) |
| 33 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_011514271.2 | 41.5% | 37.2% | (many diffs) |
| 34 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010215.2 | 41.5% | 37.2% | (many diffs) |
| 35 | human | 11119 | BTN3A1 | butyrophilin subfamily 3 me... | XR_001743132.1 | 39.5% | (many diffs) | |
| 36 | human | 11118 | BTN3A2 | butyrophilin subfamily 3 me... | XM_017010217.2 | 34.1% | 30.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1608
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaaaatggca agtttcctgg ccttccttct gctcaacttt cgtgtctgcc 121 tccttttgct tcagctgctc atgcctcact cagctcagtt ttctgtgctt ggaccctctg 181 ggcccatcct ggccatggtg ggtgaagacg ctgatctgcc ctgtcacctg ttcccgacca 241 tgagtgcaga gaccatggag ctgaagtggg tgagttccag cctaaggcag gtggtgaacg 301 tgtatgcaga tggaaaggaa gtggaagaca ggcagagtgc accgtatcga gggagaactt 361 cgattctgcg ggatggcatc actgcaggga aggctgctct ccgaatacac aacgtcacag 421 cctctgacag tggaaagtac ttgtgttatt tccaagatgg tgacttctat gaaaaagccc 481 tggtggagct gaaggttgca gcactgggtt ctgatcttca cgttgatgtg aagggttaca 541 aggatggagg gatccatctg gagtgcaggt ccactggctg gtacccccaa ccccaaatac 601 agtggagcaa caacaaggga gagaacatcc cgactgtgga agcacctgtg gttgcagacg 661 gagtgggcct gtatgcagta gcagcatctg tgatcatgag aggcagctct ggggagggtg 721 tatcctgtac catcagaagt tccctcctcg gcctggaaaa gacagccagc atttccatcg 781 cagacccctt cttcaggagc gcccagaggt ggatcgccgc cctggcaggg accctgcctg 841 tcttgctgct gcttcttggg ggagccggtt acttcctgtg gcaacagcag gaggaaaaaa 901 agactcagtt cagaaagaaa aagagagagc aagagttgag agaaatggca tggagcacaa 961 tgaagcaaga acaaagcaca agagtgaagc tcctggagga actcagatgg agaagtatcc 1021 agtatgcatc tcggggagag agacattcag cctataatga atggaaaaag gccctcttca 1081 agcctgcgga tgtgattctg gatccaaaaa cagcaaaccc catcctcctt gtttctgagg 1141 accagaggag tgtgcagcgt gccaaggagc cccaggatct gccagacaac cctgagagat 1201 ttaattggca ttattgtgtt ctcggctgtg agagcttcat atcagggaga cattactggg 1261 aggtGGAGGT AGGGGACAGG AAAGAGTGGC ATATAGGGGT GTGCAGTAAG AATGTGCAGA 1321 GAAAAGGCTG GGTCAAAATG ACACCTGAGA ATGGATTCTG GACTATGGGG CTGACTGATG 1381 GGAATAAGTA TCGGACTCTA ACTGAGCCCA GAACCAACCT GAAACTTCCT AAGCCCCCTA 1441 AGAAAGTGGG GGTCTTCCTG GACTATGAGA CTGGAGATAT CTCATTCTAC AATGCTGTGG 1501 ATGGATCGCA TATTCATACT TTCCTGGACG TCTCCTTCTC TGAGGCTCTA TATCCTGTTT 1561 TCAGAATTTT GACCTTGGAG CCCACGGCCC TGACTATTTG TCCAGCGTGA GACCCAGCTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGAGTAC GATGCCCACA CCGAACAAGC ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt