Construct: ORF TRCN0000489590

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021946.1_s317c1
DNA Barcode:
AGGCTGCTTTATAAGACAGAGCAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
EGFR (1956)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489590
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2573T>G 99.9% 100% 2361G>A;3309C>A
2 human 1956 EGFR epidermal growth factor rec... NM_005228.5 99.8% 99.9% (many diffs)
3 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2239_2247del 99.6% 99.6% (many diffs)
4 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2240_2254del 99.5% 99.5% (many diffs)
5 human 1956 EGFR epidermal growth factor rec... NM_001346899.1 96.1% 96.1% (many diffs)
6 human 1956 EGFR epidermal growth factor rec... NM_001346900.2 95.5% 95.5% (many diffs)
7 human 1956 EGFR epidermal growth factor rec... NM_001346898.2 92.6% 90.3% (many diffs)
8 human 1956 EGFR epidermal growth factor rec... NM_001346897.2 88.9% 86.5% (many diffs)
9 human 1956 EGFR epidermal growth factor rec... NM_001346941.2 77.3% 75% (many diffs)
10 human 1956 EGFR epidermal growth factor rec... NM_201284.2 56.5% 50.7% (many diffs)
11 human 1956 EGFR epidermal growth factor rec... NM_201282.2 51.8% 51.8% 1881G>A;1883C>G;1884_1885ins1746
12 human 1956 EGFR epidermal growth factor rec... NM_201283.1 33.4% 33.3% 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2414
13 mouse 13649 Egfr epidermal growth factor rec... NM_207655.2 84.6% 90.2% (many diffs)
14 mouse 13649 Egfr epidermal growth factor rec... NM_007912.4 44.9% 46.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3702
ORF length:
3630
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcgaccc tccgggacgg ccggggcagc gctcctggcg ctgctggctg
121cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc acgagtaaca
181agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg atgttcaata
241actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat tatgatcttt
301ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc aacacagtgg
361agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac gaaaattcct
421atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag gagctgccca
481tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac cctgccctgt
541gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc agcaacatgt
601cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc tgtcccaatg
661ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc atctgtgccc
721agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac aaccagtgtg
781ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa ttccgagacg
841aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc acgtaccaga
901tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag aagtgtcccc
961gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc gacagctatg
1021agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt
1081gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct acgaatatta
1141aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg gtggcattta
1201ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat attctgaaaa
1261ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac aggacggacc
1321tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat ggtcagtttt
1381ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc aaggagataa
1441gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat acaataaact
1501ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac agaggtgaaa
1561acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag ggctgctggg
1621gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg
1681acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct gagtgcatac
1741agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga cggggaccag
1801acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag acctgcccgg
1861caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc ggccatgtgt
1921gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt gaaggctgtc
1981caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc
2041tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc gttcggaagc
2101gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca cccagtggag
2161aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag
2221tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca gaaggtgaga
2281aagttaaaat tcccgtcgct atcaaggaat taagagaagc aacatctccg aaagccaaca
2341aggaaatcct cgatgaagcc tacgtgatgg ccagcgtgga caacccccac gtgtgccgcc
2401tgctgggcat ctgcctcacc tccaccgtgc aactcatcac gcagctcatg cccttcggct
2461gcctcctgga ctatgtccgg gaacacaaag acaatattgg ctcccagtac ctgctcaact
2521ggtgtgtgca gatcgcaaag ggcatgaact acttggagga ccgtcgcttg gtgcaccgcg
2581acctggcagc caggaacgta ctggtgaaaa caccgcagca tgtcaagatc acagattttg
2641ggcgggccaa actgctgggt gcggaagaga aagaatacca tgcagaagga ggcaaagtgc
2701ctatcaagtg gatggcattg gaatcaattt tacacagaat ctatacccac cagagtgatg
2761tctggagcta cggggtgacc gtttgggagt tgatgacctt tggatccaag ccatatgacg
2821gaatccctgc cagcgagatc tcctccatcc tggagaaagg agaacgcctc cctcagccac
2881ccatatgtac catcgatgtc tacatgatca tggtcaagtg ctggatgata gacgcagata
2941gtcgcccaaa gttccgtgag ttgatcatcg aattctccaa aatggcccga gacccccagc
3001gctaccttgt cattcagggg gatgaaagaa tgcatttgcc aagtcctaca gactccaact
3061tctaccgtgc cctgatggat gaagaagaca tggacgacgt ggtggatgcc gacgagtacc
3121tcatcccaca gcagggcttc ttcagcagcc cctccacgtc acggactccc ctcctgagct
3181ctctgagtgc aaccagcaac aattccaccg tggcttgcat tgatagaaat gggctgcaaa
3241gctgtcccat caaggaagac agcttcttgc agcgatacag cTCAGACCCC ACAGGCGCCT
3301TGACTGAGGA CAGCATAGAC GACACCTTCC TCCCAGTGCC TGAATACATA AACCAGTCCG
3361TTCCCAAAAG GCCCGCTGGA TCTGTGCAGA ATCCTGTCTA TCACAATCAG CCTCTGAACC
3421CCGCGCCCAG CAGAGACCCA CACTACCAGG ACCCCCACAG CACTGCAGTG GGCAACCCCG
3481AGTATCTCAA CACTGTCCAG CCCACCTGTG TCAACAGCAC ATTCGACAGC CCTGCCCACT
3541GGGCCCAGAA AGGCAGCCAC CAAATTAGCC TGGACAACCC TGACTACCAG CAGGACTTCT
3601TTCCCAAGGA AGCCAAGCCA AATGGCATCT TTAAGGGCTC CACAGCTGAA AATGCAGAAT
3661ACCTAAGGGT CGCGCCACAA AGCAGTGAAT TTATTGGAGC ATGAGACCCA GCTTTCTTGT
3721ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
3781AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
3841GAAAGGACGA AGGCTGCTTT ATAAGACAGA GCACACGCGT TAAGTCgaca atcaacctct
3901ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

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