Construct: ORF TRCN0000489651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020141.1_s317c1
- DNA Barcode:
- ACAGAGTAGCGGTATCCGCCCAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GCK (2645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489651
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2645 | GCK | glucokinase | NM_000162.5 | 100% | 100% | |
| 2 | human | 2645 | GCK | glucokinase | NM_001354800.1 | 98% | 98% | 1368_1369ins27 |
| 3 | human | 2645 | GCK | glucokinase | NM_033508.3 | 97.8% | 97.2% | (many diffs) |
| 4 | human | 2645 | GCK | glucokinase | NM_033507.3 | 97.7% | 97.4% | (many diffs) |
| 5 | human | 2645 | GCK | glucokinase | NM_001354803.1 | 29.1% | 27.3% | (many diffs) |
| 6 | human | 2645 | GCK | glucokinase | NM_001354801.1 | 27.3% | 27% | 1_1delAins1003;6_6delCinsGCAGGTGGAG |
| 7 | human | 2645 | GCK | glucokinase | XM_024446707.1 | 18.2% | 18.2% | 0_1ins1140 |
| 8 | human | 2645 | GCK | glucokinase | NM_001354802.1 | 16.3% | 16.3% | 0_1ins1140;228_229ins27 |
| 9 | mouse | 103988 | Gck | glucokinase | NM_010292.5 | 89% | 95.9% | (many diffs) |
| 10 | mouse | 103988 | Gck | glucokinase | NM_001287386.1 | 87.3% | 93.7% | (many diffs) |
| 11 | mouse | 103988 | Gck | glucokinase | XM_006514444.1 | 76.2% | 84.1% | (many diffs) |
| 12 | mouse | 103988 | Gck | glucokinase | XM_006514443.3 | 74.8% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1467
- ORF length:
- 1395
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctggac gacagagcca ggatggaggc cgccaagaag gagaaggtag 121 agcagatcct ggcagagttc cagctgcagg aggaggacct gaagaaggtg atgagacgga 181 tgcagaagga gatggaccgc ggcctgaggc tggagaccca tgaagaggcc agtgtgaaga 241 tgctgcccac ctacgtgcgc tccaccccag aaggctcaga agtcggggac ttcctctccc 301 tggacctggg tggcactaac ttcagggtga tgctggtgaa ggtgggagaa ggtgaggagg 361 ggcagtggag cgtgaagacc aaacaccaga tgtactccat ccccgaggac gccatgaccg 421 gcactgctga gatgctcttc gactacatct ctgagtgcat ctccgacttc ctggacaagc 481 atcagatgaa acacaagaag ctgcccctgg gcttcacctt ctcctttcct gtgaggcacg 541 aagacatcga taagggcatc cttctcaact ggaccaaggg cttcaaggcc tcaggagcag 601 aagggaacaa tgtcgtgggg cttctgcgag acgctatcaa acggagaggg gactttgaaa 661 tggatgtggt ggcaatggtg aatgacacgg tggccacgat gatctcctgc tactacgaag 721 accatcagtg cgaggtcggc atgatcgtgg gcacgggctg caatgcctgc tacatggagg 781 agatgcagaa tgtggagctg gtggaggggg acgagggccg catgtgcgtc aataccgagt 841 ggggcgcctt cggggactcc ggcgagctgg acgagttcct gctggagtat gaccgcctgg 901 tggacgagag ctctgcaaac cccggtcagc agctgtatga gaagctcata ggtggcaagt 961 acatgggcga gctggtgcgg cttgtgctgc tcaggctcgt ggacgaaaac ctgctcttcc 1021 acggggaggc ctccgagcag ctgcgcacac gcggagcctt cgagacgcgc ttcgtgtcgc 1081 aggtggagag cgacacgggc gaccgcaagc agatctacaa caTCCTGAGC ACGCTGGGGC 1141 TGCGACCCTC GACCACCGAC TGCGACATCG TGCGCCGCGC CTGCGAGAGC GTGTCTACGC 1201 GCGCTGCGCA CATGTGCTCG GCGGGGCTGG CGGGCGTCAT CAACCGCATG CGCGAGAGCC 1261 GCAGCGAGGA CGTAATGCGC ATCACTGTGG GCGTGGATGG CTCCGTGTAC AAGCTGCACC 1321 CCAGCTTCAA GGAGCGGTTC CATGCCAGCG TGCGCAGGCT GACGCCCAGC TGCGAGATCA 1381 CCTTCATCGA GTCGGAGGAG GGCAGTGGCC GGGGCGCGGC CCTGGTCTCG GCGGTGGCCT 1441 GTAAGAAGGC CTGTATGCTG GGCCAGTGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACAGA 1621 GTAGCGGTAT CCGCCCAATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt