Construct: ORF TRCN0000489678
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019490.1_s317c1
- DNA Barcode:
- TCCTTCGGCAATATGTCCCTGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKT3 (10000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489678
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001370074.1 | 99.9% | 99.7% | 601A>C |
| 2 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_005465.7 | 99.9% | 99.7% | 601A>C |
| 3 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001206729.1 | 95.7% | 94.1% | (many diffs) |
| 4 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_181690.2 | 95.7% | 94.1% | (many diffs) |
| 5 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446000.1 | 89.3% | 88.7% | (many diffs) |
| 6 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_016999985.1 | 87.1% | 87% | 0_1ins183;418A>C |
| 7 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446892.1 | 60.3% | 57.8% | (many diffs) |
| 8 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024447938.1 | 50.4% | 49% | (many diffs) |
| 9 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_011544014.2 | 50.2% | 42.1% | (many diffs) |
| 10 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | NM_011785.3 | 94% | 99.3% | (many diffs) |
| 11 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496818.3 | 94% | 99.3% | (many diffs) |
| 12 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496819.3 | 94% | 99.3% | (many diffs) |
| 13 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_011238805.2 | 84.3% | 85.4% | (many diffs) |
| 14 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_017320418.1 | 81.6% | 86.6% | (many diffs) |
| 15 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496821.3 | 54.1% | 52.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 1530
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgaa ttcggagtca tcatgagcga tgttaccatt gtgaaagaag 121 gttgggttca gaagagggga gaatatataa aaaactggag gccaagatac ttccttttga 181 agacagatgg ctcattcata ggatataaag agaaacctca agatgtggat ttaccttatc 241 ccctcaacaa cttttcagtg gcaaaatgcc agttaatgaa aacagaacga ccaaagccaa 301 acacatttat aatcagatgt ctccagtgga ctactgttat agagagaaca tttcatgtag 361 atactccaga ggaaagggaa gaatggacag aagctatcca ggctgtagca gacagactgc 421 agaggcaaga agaggagaga atgaattgta gtccaacttc acaaattgat aatataggag 481 aggaagagat ggatgcctct acaacccatc ataaaagaaa gacaatgaat gattttgact 541 atttgaaact actaggtaaa ggcacttttg ggaaagttat tttggttcga gagaaggcaa 601 gtggaaaata ctatgctatg aagattctga agaaagaagt cattattgca aaggatgaag 661 tggcacacac tctaactgaa agcagagtat tacagaacac tagacatccc tttttaacat 721 ccttgaaata ttccttccag acaaaagacc gtttgtgttt tgtgatggaa tatgttaatg 781 ggggcgagct gtttttccat ttgtcgagag agcgggtgtt ctctgaggac cgcacacgtt 841 tctatggtgc agaaattgtc tctgccttgg actatctaca ttccggaaag attgtgtacc 901 gtgatctcaa gttggagaat ctaatgctgg acaaagatgg ccacataaaa attacagatt 961 ttggactttg caaagaaggg atcacagatg cagccaccat gaagacattc tgtggcactc 1021 cagaatatct ggcaccagag gtgttagaag ataatgacta tggccgagca gtagactggt 1081 ggggcctagg ggttgtcatg tatgaaatga tgtgtgggag gttacctttc tacaaccagg 1141 accatgagaa acTTTTTGAA TTAATATTAA TGGAAGACAT TAAATTTCCT CGAACACTCT 1201 CTTCAGATGC AAAATCATTG CTTTCAGGGC TCTTGATAAA GGATCCAAAT AAACGCCTTG 1261 GTGGAGGACC AGATGATGCA AAAGAAATTA TGAGACACAG TTTCTTCTCT GGAGTAAACT 1321 GGCAAGATGT ATATGATAAA AAGCTTGTAC CTCCTTTTAA ACCTCAAGTA ACATCTGAGA 1381 CAGATACTAG ATATTTTGAT GAAGAATTTA CAGCTCAGAC TATTACAATA ACACCACCTG 1441 AAAAATATGA TGAGGATGGT ATGGACTGCA TGGACAATGA GAGGCGGCCG CATTTCCCTC 1501 AATTTTCCTA CTCTGCAAGT GGACGAGAAA AGGGTGGGCG CGCCGACCCA GCTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA TCCTTCGGCA ATATGTCCCT GACCACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt