Transcript: Mouse XM_006496818.3

PREDICTED: Mus musculus thymoma viral proto-oncogene 3 (Akt3), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Akt3 (23797)
Length:
7154
CDS:
427..1866

Additional Resources:

NCBI RefSeq record:
XM_006496818.3
NBCI Gene record:
Akt3 (23797)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147841 CTGCACCATAGAAACGTGTG pXPR_003 CGG 743 52% 9 0.4262 Akt3 AKT3 76220
2 BRDN0001145071 TATTTGAAACTACTAGGTAA pXPR_003 AGG 464 32% 6 0.3164 Akt3 AKT3 76219
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006496818.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000374319 TCTGCAAGCGGACGGGAATAA pLKO_005 1846 CDS 100% 13.200 18.480 N Akt3 n/a
2 TRCN0000022838 GCACCAGAGGTATTAGAAGAT pLKO.1 1366 CDS 100% 4.950 6.930 N Akt3 n/a
3 TRCN0000022835 GCTCTTGATAAAGGATCCAAA pLKO.1 1563 CDS 100% 4.950 6.930 N Akt3 n/a
4 TRCN0000366076 TGAAACAGACACCCGATATTT pLKO_005 1710 CDS 100% 15.000 12.000 N Akt3 n/a
5 TRCN0000054727 CAGCTCAGACTATTACAATAA pLKO.1 1745 CDS 100% 13.200 10.560 N Akt3 n/a
6 TRCN0000054723 CATCTGAAACAGACACCCGAT pLKO.1 1706 CDS 100% 2.160 1.728 N Akt3 n/a
7 TRCN0000374317 CCCAACCTCACAGATTGATAA pLKO_005 786 CDS 100% 13.200 9.240 N Akt3 n/a
8 TRCN0000022836 GCGTCTACAACCCATCATAAA pLKO.1 829 CDS 100% 13.200 9.240 N Akt3 n/a
9 TRCN0000001612 GCTGCTACTGTCTTACTATTA pLKO.1 2096 3UTR 100% 13.200 9.240 N AKT3 n/a
10 TRCN0000010184 GTAAACTGGCAAGATGTATAT pLKO.1 1648 CDS 100% 13.200 9.240 N AKT3 n/a
11 TRCN0000366075 TGCGTCTACAACCCATCATAA pLKO_005 828 CDS 100% 13.200 9.240 N Akt3 n/a
12 TRCN0000366074 TGGCTCATTCATAGGCTATAA pLKO_005 522 CDS 100% 13.200 9.240 N Akt3 n/a
13 TRCN0000022834 CCACTGTTATAGAGAGAACAT pLKO.1 665 CDS 100% 4.950 3.465 N Akt3 n/a
14 TRCN0000054724 CTATGCTATGAAGATTCTGAA pLKO.1 945 CDS 100% 4.950 3.465 N Akt3 n/a
15 TRCN0000054725 CCGTGATCTCAAGTTGGAGAA pLKO.1 1233 CDS 100% 4.050 2.835 N Akt3 n/a
16 TRCN0000039888 CCTCATCTTTCTCCTTCATTA pLKO.1 3406 3UTR 100% 13.200 7.920 N AKT3 n/a
17 TRCN0000374318 GCTACTGTCTTACTATTATAG pLKO_005 2099 3UTR 100% 13.200 7.920 N Akt3 n/a
18 TRCN0000022837 GATGGCTCATTCATAGGCTAT pLKO.1 520 CDS 100% 4.050 2.430 N Akt3 n/a
19 TRCN0000001616 AGAAACCTCAAGATGTGGATT pLKO.1 545 CDS 100% 4.950 3.465 N AKT3 n/a
20 TRCN0000001614 ACTGGCAAGATGTATATGATA pLKO.1 1652 CDS 100% 5.625 3.375 N AKT3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006496818.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489669 GCGGGCGGAAGTCATGTGCGCTCC pLX_317 28.3% 94% 99.5% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000487677 GGAGAAATTATCCTCAACCATTAC pLX_317 9% 94% 99.5% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000489678 TCCTTCGGCAATATGTCCCTGACC pLX_317 26.8% 94% 99.3% V5 (many diffs) n/a
4 ccsbBroadEn_14950 pDONR223 100% 93.1% 97% None (many diffs) n/a
5 ccsbBroad304_14950 pLX_304 35.9% 93.1% 97% V5 (many diffs) n/a
6 TRCN0000470264 CTGCTGTTGCGGCCGTAACAACGT pLX_317 19.2% 93.1% 97% V5 (many diffs) n/a
7 ccsbBroadEn_07529 pDONR223 100% 89.9% 94.5% None (many diffs) n/a
8 ccsbBroad304_07529 pLX_304 45.1% 89.9% 94.5% V5 (many diffs) n/a
9 TRCN0000473926 TGTAACGCGCAGGATGTGGCCCAG pLX_317 34.7% 89.9% 94.5% V5 (many diffs) n/a
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