Transcript: Mouse XM_017320418.1

PREDICTED: Mus musculus thymoma viral proto-oncogene 3 (Akt3), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Akt3 (23797)
Length:
6839
CDS:
295..1551

Additional Resources:

NCBI RefSeq record:
XM_017320418.1
NBCI Gene record:
Akt3 (23797)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017320418.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000374319 TCTGCAAGCGGACGGGAATAA pLKO_005 1531 CDS 100% 13.200 18.480 N Akt3 n/a
2 TRCN0000022838 GCACCAGAGGTATTAGAAGAT pLKO.1 1051 CDS 100% 4.950 6.930 N Akt3 n/a
3 TRCN0000022835 GCTCTTGATAAAGGATCCAAA pLKO.1 1248 CDS 100% 4.950 6.930 N Akt3 n/a
4 TRCN0000366076 TGAAACAGACACCCGATATTT pLKO_005 1395 CDS 100% 15.000 12.000 N Akt3 n/a
5 TRCN0000054727 CAGCTCAGACTATTACAATAA pLKO.1 1430 CDS 100% 13.200 10.560 N Akt3 n/a
6 TRCN0000054723 CATCTGAAACAGACACCCGAT pLKO.1 1391 CDS 100% 2.160 1.728 N Akt3 n/a
7 TRCN0000374317 CCCAACCTCACAGATTGATAA pLKO_005 471 CDS 100% 13.200 9.240 N Akt3 n/a
8 TRCN0000022836 GCGTCTACAACCCATCATAAA pLKO.1 514 CDS 100% 13.200 9.240 N Akt3 n/a
9 TRCN0000001612 GCTGCTACTGTCTTACTATTA pLKO.1 1781 3UTR 100% 13.200 9.240 N AKT3 n/a
10 TRCN0000010184 GTAAACTGGCAAGATGTATAT pLKO.1 1333 CDS 100% 13.200 9.240 N AKT3 n/a
11 TRCN0000366075 TGCGTCTACAACCCATCATAA pLKO_005 513 CDS 100% 13.200 9.240 N Akt3 n/a
12 TRCN0000366074 TGGCTCATTCATAGGCTATAA pLKO_005 207 5UTR 100% 13.200 9.240 N Akt3 n/a
13 TRCN0000022834 CCACTGTTATAGAGAGAACAT pLKO.1 350 CDS 100% 4.950 3.465 N Akt3 n/a
14 TRCN0000054724 CTATGCTATGAAGATTCTGAA pLKO.1 630 CDS 100% 4.950 3.465 N Akt3 n/a
15 TRCN0000054725 CCGTGATCTCAAGTTGGAGAA pLKO.1 918 CDS 100% 4.050 2.835 N Akt3 n/a
16 TRCN0000039888 CCTCATCTTTCTCCTTCATTA pLKO.1 3091 3UTR 100% 13.200 7.920 N AKT3 n/a
17 TRCN0000374318 GCTACTGTCTTACTATTATAG pLKO_005 1784 3UTR 100% 13.200 7.920 N Akt3 n/a
18 TRCN0000022837 GATGGCTCATTCATAGGCTAT pLKO.1 205 5UTR 100% 4.050 2.430 N Akt3 n/a
19 TRCN0000001616 AGAAACCTCAAGATGTGGATT pLKO.1 230 5UTR 100% 4.950 3.465 N AKT3 n/a
20 TRCN0000001614 ACTGGCAAGATGTATATGATA pLKO.1 1337 CDS 100% 5.625 3.375 N AKT3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017320418.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489669 GCGGGCGGAAGTCATGTGCGCTCC pLX_317 28.3% 81.6% 86.8% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000487677 GGAGAAATTATCCTCAACCATTAC pLX_317 9% 81.6% 86.8% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000489678 TCCTTCGGCAATATGTCCCTGACC pLX_317 26.8% 81.6% 86.6% V5 (many diffs) n/a
4 ccsbBroadEn_14950 pDONR223 100% 80.9% 84.5% None (many diffs) n/a
5 ccsbBroad304_14950 pLX_304 35.9% 80.9% 84.5% V5 (many diffs) n/a
6 TRCN0000470264 CTGCTGTTGCGGCCGTAACAACGT pLX_317 19.2% 80.9% 84.5% V5 (many diffs) n/a
7 ccsbBroadEn_07529 pDONR223 100% 77.6% 81.9% None (many diffs) n/a
8 ccsbBroad304_07529 pLX_304 45.1% 77.6% 81.9% V5 (many diffs) n/a
9 TRCN0000473926 TGTAACGCGCAGGATGTGGCCCAG pLX_317 34.7% 77.6% 81.9% V5 (many diffs) n/a
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