Construct: ORF TRCN0000489766

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019797.1_s317c1
DNA Barcode:
AGCCGATCCTTTATGCTTTCGTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NADK2 (133686)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489766
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 133686 NADK2 NAD kinase 2, mitochondrial NM_001287340.1 99.8% 99.6% 837_838insG
2 human 133686 NADK2 NAD kinase 2, mitochondrial NM_153013.4 99.8% 99.6% 837_838insG
3 human 133686 NADK2 NAD kinase 2, mitochondrial NM_001287341.1 92.5% 92.3% 372_437del;903_904insG
4 human 133686 NADK2 NAD kinase 2, mitochondrial XM_024454360.1 92.5% 92.3% 372_437del;903_904insG
5 human 133686 NADK2 NAD kinase 2, mitochondrial NM_001085411.3 63% 62.9% 1_489del;1326_1327insG
6 human 133686 NADK2 NAD kinase 2, mitochondrial XM_005248241.4 60% 60% 1_489del;861_926del;1392_1393insG
7 mouse 68646 Nadk2 NAD kinase 2, mitochondrial NM_001040395.4 57.9% 61.7% (many diffs)
8 mouse 68646 Nadk2 NAD kinase 2, mitochondrial NM_001085410.2 55.1% 58.7% (many diffs)
9 mouse 68646 Nadk2 NAD kinase 2, mitochondrial NM_001286255.1 52.3% 55.9% (many diffs)
10 mouse 68646 Nadk2 NAD kinase 2, mitochondrial NM_001286253.1 48.3% 51.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
909
ORF length:
840
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gctgctggca gcgagtaaag tcttggacag acttaaacca gttatagggg
121taaacactga tccagaacgg tctgagggtc atttatgcct gcccgttcga tatacacatt
181cctttccaga agccttacag aagttctatc gtggtgagtt caggtggttg tggaggcaga
241gaatcaggtt ataccttgaa gggactggca taaaccctgt acctgtggac cttcacgagc
301agcagctaag cttgaatcag cacaatagag cccttaacat tgaaagagct catgatgaaa
361ggtctgaggc ttcaggaccc caacttctgc cagtgagagc actaaatgaa gtcttcattg
421gggagagtct gtcatccagg gcttcctact atgagatttc agttgatgat ggtccatggg
481aaaaACAGAA GAGTTCAGGG CTCAATTTGT GTACTGGAAC AGGATCAAAG GCCTGGTCAT
541TCAATATTAA CAGGGTTGCA ACTCAGGCTG TAGAAGATGT TTTAAATATT GCAAAACGAC
601AAGGAAATTT GAGTCTTCCA TTGAACAGAG AATTGGTAGA GAAAGTAACA AATGAATATA
661ATGAATCACT GCTCTACAGT CCGGAAGAAC CAAAAATACT TTTCAGTATT CGAGAACCAA
721TAGCAAATAG AGTTTTCTCA AGCAGTCGTC AGCGTTGTTT CTCCTCAAAG GTTTGTGTTC
781GTTCTCGTTG TTGGGATGCC TGTATGGTTG TGGATGGAGG AACTTCTTTT GAGTTTAATG
841ATGGTGCAAT TGCTTCGATG ATGATCAATA AAGAAGATGA GCTTCGAACT GTGCTTCTTG
901AACAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
961TCCTCGGTCT CGATTCTACG TAGTAATGAA CTTTATATAT CTTGTGGAAA GGACGAAGCC
1021GATCCTTTAT GCTTTCGTAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt
1081gtgaaagatt

Download FASTA (ORF) (Full)