Construct: ORF TRCN0000489766
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019797.1_s317c1
- DNA Barcode:
- AGCCGATCCTTTATGCTTTCGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NADK2 (133686)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489766
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | NM_001287340.1 | 99.8% | 99.6% | 837_838insG |
| 2 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | NM_153013.4 | 99.8% | 99.6% | 837_838insG |
| 3 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | NM_001287341.1 | 92.5% | 92.3% | 372_437del;903_904insG |
| 4 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | XM_024454360.1 | 92.5% | 92.3% | 372_437del;903_904insG |
| 5 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | NM_001085411.3 | 63% | 62.9% | 1_489del;1326_1327insG |
| 6 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | XM_005248241.4 | 60% | 60% | 1_489del;861_926del;1392_1393insG |
| 7 | mouse | 68646 | Nadk2 | NAD kinase 2, mitochondrial | NM_001040395.4 | 57.9% | 61.7% | (many diffs) |
| 8 | mouse | 68646 | Nadk2 | NAD kinase 2, mitochondrial | NM_001085410.2 | 55.1% | 58.7% | (many diffs) |
| 9 | mouse | 68646 | Nadk2 | NAD kinase 2, mitochondrial | NM_001286255.1 | 52.3% | 55.9% | (many diffs) |
| 10 | mouse | 68646 | Nadk2 | NAD kinase 2, mitochondrial | NM_001286253.1 | 48.3% | 51.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 909
- ORF length:
- 840
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gctgctggca gcgagtaaag tcttggacag acttaaacca gttatagggg 121 taaacactga tccagaacgg tctgagggtc atttatgcct gcccgttcga tatacacatt 181 cctttccaga agccttacag aagttctatc gtggtgagtt caggtggttg tggaggcaga 241 gaatcaggtt ataccttgaa gggactggca taaaccctgt acctgtggac cttcacgagc 301 agcagctaag cttgaatcag cacaatagag cccttaacat tgaaagagct catgatgaaa 361 ggtctgaggc ttcaggaccc caacttctgc cagtgagagc actaaatgaa gtcttcattg 421 gggagagtct gtcatccagg gcttcctact atgagatttc agttgatgat ggtccatggg 481 aaaaACAGAA GAGTTCAGGG CTCAATTTGT GTACTGGAAC AGGATCAAAG GCCTGGTCAT 541 TCAATATTAA CAGGGTTGCA ACTCAGGCTG TAGAAGATGT TTTAAATATT GCAAAACGAC 601 AAGGAAATTT GAGTCTTCCA TTGAACAGAG AATTGGTAGA GAAAGTAACA AATGAATATA 661 ATGAATCACT GCTCTACAGT CCGGAAGAAC CAAAAATACT TTTCAGTATT CGAGAACCAA 721 TAGCAAATAG AGTTTTCTCA AGCAGTCGTC AGCGTTGTTT CTCCTCAAAG GTTTGTGTTC 781 GTTCTCGTTG TTGGGATGCC TGTATGGTTG TGGATGGAGG AACTTCTTTT GAGTTTAATG 841 ATGGTGCAAT TGCTTCGATG ATGATCAATA AAGAAGATGA GCTTCGAACT GTGCTTCTTG 901 AACAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 961 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTTTATATAT CTTGTGGAAA GGACGAAGCC 1021 GATCCTTTAT GCTTTCGTAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt 1081 gtgaaagatt